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ORIGINAL ARTICLE
Year : 2018  |  Volume : 10  |  Issue : 3  |  Page : 309-313

Evaluation of anticancer activity of methanolic extract of Hiptage benghalensis (L.) Kurz on Cancer Cell Lines


Department of Pharmacology, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Telangana, India

Correspondence Address:
Narsimha Reddy Yellu
Department of Pharmacology, University College of Pharmaceutical Sciences, Kakatiya University, Warangal - 506 009, Telangana
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pr.pr_102_17

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Aim and Background: This study aimed to study the anticancer effects of Hiptage benghalensis (L.) kurz using various human cancer cell cultures, in vitro using 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay. Materials and Methods: Human cervical carcinoma (HeLa) cells, human breast cancer (MCF-7) cells, and human neuroblastoma (IMR-32) cells were maintained in a 5% CO2 incubator at 37°C. Different concentrations of an extract of H. benghalensis in serum-free culture medium were freshly prepared and used for cytotoxic activity using MTT assay activity. Results: Among the plant extract had revealed that, the greater percentage inhibition in all types of cancer cells in a dose-dependent manner using MTT assay. The concentration of the extract causing 50% cell death values of methanolic extract of H. benghalensis were found to be 50.73, 47.90, and 53.76 μg/mL against HeLa, MCF-7, and IMR-32, respectively. Conclusion: H. benghalensis methanolic extract were showed increased percentage inhibition of MCF 7, HeLa, and IMR 32 cells using MTT assay. Abbreviation Used: HBM: Hiptage benghalensis Methanolic extract, MTT: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide, MCF 7: Human breast cancer cells, HeLa: Human cervical carcinoma cells, IMR 32: Human neuroblastoma, H2DCFDA: 2',7' dichlorodihydrofluorescein diacetate, PBS: Phosphate-buffered saline, DMSO: Dimethyl sulfoxide, DCF: Dichlorofluorescein, ROS: Reactive oxygen species.


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