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SHORT COMMUNICATION
Year : 2018  |  Volume : 10  |  Issue : 2  |  Page : 230-235

Gas chromatography-mass spectrometric determination of components of leaves of Aegle marmelos and Psidium guajava and seeds of Nigella sativa and correlation with In vitro antioxidant activity


1 Department of Pharmacology, Delhi Institute of Pharmaceutical Science and Research, New Delhi, India
2 Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Bioactive Natural Product Laboratory, Jamia Hamdard, New Delhi, India

Correspondence Address:
Dr. Rajani Mathur
Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec III, MB Road, New Delhi - 110 017
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pr.pr_93_17

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Objective: The aim of this study is to analyze the volatile components of the leaves of Aegle marmelos, Psidium guajava, and seeds of Nigella sativa, using gas chromatography-mass spectrometric (GC-MS) and correlate with their in vitro antioxidant activity. Materials and Methods: Plant material was analyzed for heavy metal content (HMC), pesticide residue (PR), and the presence of microbes/fungi. Following hydrodistillation, the volatile components (AM, PG, and NS) were subjected to GC-MS analysis and in vitro antioxidant assay (2,2-diphenyl-1-picrylhydrazyl). Results: The plant material passed the test for HMC, PR, microbial, and fungal contamination. Using GC-MS, the number of compounds identified in AM, PG, and NS were 62, 46, and 58, respectively. The antioxidant activity of AM, PG, and NS was 98.538%, 98.955%, and 97.755%, respectively. Conclusion: GC-MS-based methods can be successfully utilized for phytochemical profiling and standardization of plant material. Abbreviations Used: AM: Volatile oil of Aegle marmelos Correa.; GC-MS: Gas chromatography-mass spectrometry; HMC: Heavy metal content; NS: Volatile oil of Nigella sativa Linn.; PG: Volatile oil of Psidium guajava Linn.; PR: Pesticide residue.


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