ORIGINAL ARTICLE |
|
Year : 2017 | Volume
: 9
| Issue : 5 | Page : 5-8 |
|
The activity of immunoglobulin Y anti-Mycobacterium tuberculosis on proliferation and cytokine expression of rat peripheral blood mononuclear cells
Sri Agus Sudjarwo1, Koerniasari Eraiko2, Giftania Wardani Sudjarwo3, Koerniasari4
1 Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115, Indonesia 2 Department of Conservative Dentistry, Faculty of Dentistry, Airlangga University, Surabaya 60115, Indonesia 3 Department of Pharmacy Biology, Faculty of Pharmacy, Hang Tuah University, Surabaya 60115, Indonesia 4 Department Microbiology, Study Program of Environmental Health, Polytechnic of Health, Surabaya 60115, Indonesia
Correspondence Address:
Sri Agus Sudjarwo Department of Pharmacology, Faculty of Veterinary Medicine, Airlangga University, Surabaya 60115 Indonesia
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/pr.pr_66_17
|
|
Objective: It has long been known that chickens, like mammals, are capable of producing antigen-specific immunoglobulin Y (IgY), which functions similar to IgG. The present study was performed to investigate the activity of IgY anti-Mycobacterium tuberculosis on proliferation, interleukin (IL)-2, and interferon (IFN)-γ expression of rat peripheral blood mononuclear cells (PBMCs). Materials and Methods: The activity of IgY anti-M. tuberculosis in different doses (25, 50, and 100 μg/ml) on rat PBMCs proliferation was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. The production of IL-2 and IFN-γ in the PBMC supernatant was determined using enzyme-linked immunosorbent assay. Investigation was performed on mRNA expression of IL-2 and IFN-γ by reverse transcription-polymerase chain reaction (RT-PCR). Results: IgY anti-M. tuberculosis significantly increased the proliferation of rat PBMC. Furthermore, IgY anti-M. tuberculosis dose dependently increased IL-2 and IFN-γ production in PBMC, suggesting that pharmacological activities of IgY anti-M. tuberculosis in PBMC may be mediated by regulating the production of cytokines. In the RT-PCR, expression of cytokines such as IL-2 and IFN-γ in PBMC cultures was increased by IgY anti-M. tuberculosis. Conclusions: We concluded that increasing IL-2 and IFN-γ productions in PBMC was related to IgY anti-M. tuberculosis, stimulating the mRNA transcription (gene expression) of these cytokines which can induce proliferation of PBMC. |
|
|
|
[FULL TEXT] [PDF]* |
|
 |
|