| ORIGINAL ARTICLE |
|
| Year : 2017 | Volume
: 9
| Issue : 5 | Page : 34-40 |
|
|
Pharmacological screening of Trachyspermum ammi for antihyperlipidemic activity in Triton X-100 induced hyperlipidemia rat model
Uzma Saleem1, Saba Riaz1, Bashir Ahmad2, Saleem Mohammad1
1 Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Pakistan
2 Riphah Institute of Pharmaceutical Sciences, Riphah International University, Lahore Campus, Pakistan
Correspondence Address:
Uzma Saleem
Faculty of Pharmaceutical Sciences, Government College University, Faisalabad
Pakistan
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/pr.pr_37_17
|
|
|
Background: Mortality rate is increasing due to cardiovascular problems throughout the world. These cardiac problems are directly associated with dyslipidemia. Aim: The aim of this study was to evaluate the antihyperlipidemic effect of aqueous extract and methanol extract of Trachyspermum ammi at 1 g/kg, 3 g/kg, and 5 g/kg dose levels in rats. Materials and Methods: For this purpose, 45 male albino rats were used and randomly divided into nine equal groups (n = 5). The lipid levels were increased after 24 h of single intraperitoneal injection of Triton X-100 (100 mg/kg) in rats. Aqueous and methanol extracts equivalent to 1 g/kg, 3 g/kg, and 5 g/kg were administered orally to the rats for 21 days. Atorvastatin (10 mg/kg) was used as standard drug. Blood samples were collected at 0, 2nd, 9th, 16th, and 23rd day by a direct cardiac puncture in Vacuette® heparin tubes. Serum was separated and then analyzed for lipid profile, liver function test (LFT), and renal function test (RFT) using standard diagnostic kits. Results: Results showed that extracts at 3 g/kg and 5 g/kg decreased the levels of total cholesterol, triglyceride, and low-density lipoprotein and increased high-density lipoprotein concentration in serum. T. ammi also decreased LFT and RFT parameters at the end of the study. Conclusion: T. ammi possessed antioxidant and antihyperlipidemic activities along with hepato- and nephro-protective effects. Aqueous and methanol extracts of T. ammi were administered orally at 1-, 3-, and 5 g/kg doses to hyperlipidemic rats (Triton X-100 induced hyperlipidemia) and atorvastatin (10 mg/kg, orally) was used as standard drug. Methanol extract at 5 g/kg showed antihyperlipidemic effect that is identical to that of standard drug. |
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
