Home | About PR | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |   Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2017  |  Volume : 9  |  Issue : 1  |  Page : 27-33

Chronic intake of green propolis negatively affecting the rat testis

1 Reproductive Biology Laboratory, Department of Structural and Functional Biology, Biology Institute, State University of Campinas – UNICAMP, Campinas, Brazil
2 Heath Sciences Nucleus, Hermínio Ometto Univerity Center, UNIARARAS, Araras, São Paulo, Brazil
3 Graduate Program in Biomedical Sciences, Hermínio Ometto University Center, UNIARARAS, Araras, São Paulo, Brazil
4 Department of Biological Sciences, State University of Paraná (UNESPAR), Paranaguá, Paraná, Brazil

Correspondence Address:
Grasiela Dias de Campos Severi-Aguiar
Departamento de Biologia Estrutural e Funcional, Universidade Estadual de Campinas – UNICAMP, CP 6109, CEP 13083-863, Campinas, São Paulo
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0974-8490.199777

Rights and Permissions

Background: Human and animal evidence suggests that environmental toxicants may have an adverse impact on male reproductive health, reducing the population's reproductive output. Owing to the renewed attraction for natural products, some of them constitute effective alternatives to mitigate these effects. Propolis is a candidate for this use because of its intrinsic properties. In many situations, it improved the testicular damage and alleviated the toxic effects induced by environmental contaminant exposure. Objective: The aim of this study was to investigate possible alterations of testicular parameters and certify if its use is really advantageous to the testis, since this could affect rat reproductive function. Materials and Methods: Forty-eight adult male Wistar rats were divided into four groups (Co = control, T1 = 3 mg propolis/kg/day, T2 = 6 mg/kg/day, T3 = 10 mg/kg/day) and were exposed during 56 days. The testes were assessed with morphometrical, stereological, and ultrastructural analyses. Cell proliferation and death were diagnosed, respectively, by immunocytochemistry. Connexin 43 (Cx43) and N-cadherin transcript levels were determined by reverse transcription-polymerase chain reaction. Results: Increased cell proliferation and Leydig cell volume were observed in T2, and in contrast, Cx43 upregulation and cell death were observed in T3. Both T2 and T3 showed ultrastructural abnormalities in testicular parenchyma. Conclusion: We recommend a cautious intake of propolis to avoid deleterious effects.

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded83    
    Comments [Add]    

Recommend this journal