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ORIGINAL ARTICLE
Year : 2016  |  Volume : 8  |  Issue : 4  |  Page : 281-286

Elucidation of flavonoids from Carissa congesta, Polyalthia longifolia, and Benincasa hispida plant extracts by hyphenated technique of liquid chromatography-mass spectroscopy


1 Department of Pharmacology and Pharmaceutical Chemistry, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra; Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India
2 Department of Pharmacology and Pharmaceutical Chemistry, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India
3 Department of Quality Assurance, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India
4 Department of Pharmaceutical Chemistry, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra, India
5 Department of Pharmacology, Y. B. Chavan College of Pharmacy, Rouzabagh, Aurangabad, India

Correspondence Address:
Gaurav M Doshi
Department of Pharmacology, Vivekanand Education Society's College of Pharmacy, Mumbai, Maharashtra
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-8490.186578

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Background: Carissa congesta (CC), Polyalthia longifolia (PL), and Benincasa hispida (BH) are economically important plants. Objective: Current research encompasses identification of quercetin and rutin and their analogues by liquid chromatography-mass spectroscopy (LC-MS) from the selected plant species. Materials and Methods: Fresh roots, leaves, and seeds of CC, PL, and BH plants respectively were shade-dried followed by extraction and elucidation of rutin and quercetin by LC-MS. Results: Structural elucidation of CC, PL, and BH extracts revealed the presence of flavonoids such as quercetin (m/z 301) and rutin (m/z 610) as the parent ions along with presence of close analogues such as quercetin-O-hexoside, Vicenin 2, quercetin-3-O-xyloside/arabinoside, and quercetin-3-O-glucoside were identified as fragments. Conclusions: Thus, CC, PL, and BH extracts revealed the presence of flavonoids belonging to the class of flavonols such as rutin and quercetin.


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