|Year : 2016 | Volume
| Issue : 3 | Page : 202-205
Croton grewioides Baill. (Euphorbiaceae) shows antidiarrheal activity in mice
Anne Dayse Soares da Silva1, Karoline de Melo e Silva2, José Clementino Neto2, Vicente Carlos de Oliveira Costa3, Hilzeth de Luna F Pessôa4, Josean Fechine Tavares5, Marcelo Sobral da Silva5, Fabiana de Andrade Cavalcante6
1 Postgraduate Program in Natural Products and Synthetic Bioactive, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil
2 Institute of Biological Sciences and Health, Federal University of Alagoas, Maceió 57020-720, AL, Brazil
3 Institute for Research on Drugs and Medicines, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil
4 Department of Molecular Biology, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil
5 Postgraduate Program in Natural Products and Synthetic Bioactive; Department of Pharmaceutical Sciences, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil
6 Postgraduate Program in Natural Products and Synthetic Bioactive; Department of Physiology and Pathology, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil
|Date of Web Publication||24-May-2016|
Fabiana de Andrade Cavalcante
Postgraduate Program in Natural Products and Synthetic Bioactive, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil. Department of Physiology and Pathology, Federal University of Paraíba, João Pessoa 58051-900, PB
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Based on chemotaxonomy, we decided to investigate the possible antidiarrheal activity in mice of a crude ethanolic extract obtained from aerial parts of Croton grewioides (CG-EtOH). We tested for any possible toxicity in rat erythrocytes and acute toxicity in mice. Antidiarrheal activity was assessed by determining the effect of CG-EtOH on defecation frequency, liquid stool, intestinal motility and intestinal fluid accumulation. CG-EtOH showed no in vitro cytotoxicity and was not orally lethal. In contrast, the extract given intraperitoneally (at 2000 mg/kg) was lethal, but only in females. CG-EtOH produced a significant and equipotent antidiarrheal activity, both in defecation frequency (ED50 = 106.0 ± 8.1 mg/kg) and liquid stools (ED50 = 105.0 ± 9.2 mg/kg). However, CG-EtOH (125 mg/kg) decreased intestinal motility by only 22.7% ±4.4%. Moreover, extract markedly inhibited the castor oil-induced intestinal contents (ED50 = 34.6 ± 5.4 mg/kg). We thus conclude that CG-EtOH is not orally lethal and contains active principles with antidiarrheal activity, and this effect seems to involve mostly changes in intestinal secretion.
- CG-EtOH showed no in vitro cytotoxicity and was not orally lethal. In contrast the extract given intraperitoneally (at 2000 mg/kg) was lethal, but only in females.
- CG-EtOH probably contains active metabolites with antidiarrheal activity.
- CG-EtOH reduced the frequency and number of liquid stools.
- Metabolites presents in the CG-EtOH act mainly by reducing intestinal fluid and, to a lesser extent, reducing intestinal motility.
Abbreviations Used: CG-EtOH: crude ethanolic extract obtained from the aerial parts of C. grewioides; WHO: World Health Organization; ED50: dose of a drug that produces 50% of its maximum effect; Emax: maximum effect
Keywords: Croton grewioides, Diarrheal, Intestinal fluid, Motility, Toxicity
|How to cite this article:|
da Silva AD, de Melo e Silva K, Neto JC, Costa VC, Pessôa HF, Tavares JF, da Silva MS, Cavalcante Fd. Croton grewioides Baill. (Euphorbiaceae) shows antidiarrheal activity in mice. Phcog Res 2016;8:202-5
|How to cite this URL:|
da Silva AD, de Melo e Silva K, Neto JC, Costa VC, Pessôa HF, Tavares JF, da Silva MS, Cavalcante Fd. Croton grewioides Baill. (Euphorbiaceae) shows antidiarrheal activity in mice. Phcog Res [serial online] 2016 [cited 2021 Feb 26];8:202-5. Available from: http://www.phcogres.com/text.asp?2016/8/3/202/181465
| Introduction|| |
The world's population uses different ways to treat, cure and prevent diseases, and among these tools, medicinal plants play an important part, because it has a broad and diverse application, which has been reported since the beginning of humanity. Brazil has one of the richest floras on the planet, and due to different environments, it is estimated that of the 1.4 million of described organisms on the planet, around 10% occur in Brazil.
Euphorbiaceae is one of the largest families of Phanerogams comprising about 300 genera and 7600 species. In this family, the genus Croton is the second largest, with about 1200 species, distributed in tropical and subtropical regions. This genus is of significant economic relevance since several species are used as a source of raw materials for the chemical and pharmaceutical industries. In addition, previous studies showed its species contain various active substances such as terpenoids, flavonoids, and alkaloids with various biological activities as, e.g., anti-inflammatory, cytotoxic, antirheumatic, antiulcer, analgesic, and antispasmodic activity.,,,,,
Croton grewioides (CG) Baill. (Euphorbiaceae) is known as “Canelinha” or “Canelinha-de-cheiro” in allusion to the fragrance given off by its flowers. In some regions of Northeast Brazil, the nectar of its flowers is much appreciated for its taste and aroma.
The need for the discovery and research of plant compounds that act on symptoms of diarrhea and not just in regard to dehydration  has prompted pharmacological studies of the plant CG using a chemotaxonomic approach, since other Croton species have antidiarrheal properties, such as Croton cajucara,Croton Tiglium, and Croton urucurana.
According to the World Health Organization, acute diarrhea is characterized by an increase in the number of stools (3 or more in a 24 h period) may be aqueous or low consistency or even an increase in stool frequency compared to what is normal for each individual. It is the second leading cause of death in children under 5 years of age, with approximately 760,000 deaths/year, in addition to being a major cause of malnutrition in the same group on a global scale and reach about 1.7 billion people by year, therefore, may be a world problem., The liquid content is the main determinant of the volume and consistency of stools, but other factors also contribute to the formation of the stool, such as the propulsion rate. In Northeast Brazil, persistent diarrhea associated with acute malnutrition remains the major cause of morbidity and mortality.
Therefore, we decided to investigate the antidiarrheal activity of a crude ethanolic extract obtained from the aerial parts of GC-EtOH in mice.
| Materials and Methods|| |
Aerial parts of CG were collected in the municipality of Serra Branca, State of Paraíba, in March 2007. The botanical material was identified by Professor Maria de Fatima Agra, and a voucher specimen of the plant is deposited in the Herbarium Prof. Lauro Pires Xavier (JPB), Federal University of Paraíba identified as Agra et al. 6987. Plant material was dried for 3–4 days at 45°C, and then pulverized in a mill. The powder of the aerial parts (2500 g) was sprayed dried and subjected to thorough maceration with 95% ethanol, in a stainless steel container for 72 h. This step was repeated 4 times. The extraction solution was concentrated by rotary evaporation under reduced pressure at a temperature of 45°C to give the crude ethanolic extract (116.28 g), yield 4.65% on dry weight of the plant. The phytochemical studies of the CG aerial parts were performed by standard chromatographic methods and identified by spectroscopic methods such as infrared, mass,1 H NMR,13 C NMR and two-dimensional beyond compare with literature data. This phytochemical study resulted in the isolation of 13 compounds, three- first described in the literature, two isolated for the 1st time in the genus and eight- first reported in the species.
Atropine (Sigma-Aldrich, USA) was used as a positive control in the experiments of transit and fluid intestinal; Cremophor ® (Sigma-Aldrich, USA) as vehicle and Triton X-100 (Sigma-Aldrich, USA) as the hemolytic agent. Carboxymethyl cellulose or cellulose gum, is used as a viscosity modifier or thickener, and castor oil as stimulant laxative, both obtained from formula, Brazil. Loperamide was used as antidiarrheal standard drug (J. C. F. Ltda, Brazil), and the activated charcoal as a marker of intestinal transit obtained from Proquímios, Brazil.
Female and male Swiss mice (Mus musculus) and Wistar rat (Rattus norvegicus) from Central Animal Laboratory, Federal University of Paraíba (UFAL), weighing between 25 and 35 g, were used. All experimental procedures with CG were approved by the Ethical Committee in Research of UFAL (No. 010489/2009 15).
Toxicological evaluation: Effect of Croton grewioides-EtOH on rat erythrocytes
Rats (200–300 g) were fasted for a period of 12 h. Afterward, a blood sample was collected, mixed with a solution of NaCl (0.9%) and CaCl2 (10 mM) and centrifuged at 5000 rpm/3 min. The extract was added to the erythrocyte suspension at various concentrations. The negative control consisted of the erythrocyte suspension in NaCl and CaCl2 solution and positive control as erythrocyte suspension plus 100 mL of Triton X-100 1%. Hemolysis was quantified by spectrophotometry at 540 nm.
Pharmacological behavioral screening and investigation of acute toxicity of Croton grewioides-EtOH
CG-EtOH was administered orally (p.o.) or intraperitoneally (i.p.) to groups of five males and females (one dose/group), which were fasted for 12 h. Simultaneously, control animals received 10 mL/kg saline plus Cremophor ®. General signs and symptoms of toxicity, such as analgesia, contortions, and aggression, were recorded for (4 h). Death of animals was determined during 72 h.
Evaluation of antidiarrheal activity of the Croton grewioides-EtOH
Castor oil-induced diarrhea in mice
Male mice were gavaged with 10 mL/kg saline plus Cremophor ® (negative control), 10 mg/kg loperamide (positive control) or CG-EtOH. After 1 h, 0.01 mL of castor oil per gram body weight was administered orally to each animal to induce diarrhea, and the counts and consistency of dung pats were determined for 4 h, classifying them according to consistency as solids or liquids.
Intestinal transit in mice
Male mice were gavaged with 10 mL/kg saline plus Cremophor ® (negative control), 2 mg/kg atropine (positive control) or CG-EtOH. After 30 min, 0.01 mL of activated charcoal (5%) in carboxymethyl cellulose (0.5%) was administered orally per gram body weight to each animal, which was euthanized by cervical dislocation after 30 min. The abdominal cavity was opened and the small intestine removed. The distance traveled by the marker was assessed for each animal. The results were expressed as a percentage of the distance traveled by the marker in relation to the total length.
Intestinal fluid accumulation induced by castor oil in mice
Male mice (fasting for 24 h) were divided into groups: 10 mL/kg saline plus Cremophor ®; 10 mg/kg loperamide and CG-EtOH. After 30 min, 2 mL of castor oil was administered to each animal. After another 30 min, the mice were euthanized, the small intestine was dissected from the pylorus throughout the cecum, and the contents were collected in a beaker to assess the volume of fluid.
For all the above experiments, results were expressed as mean ± standard error of the mean statistical significance tests were performed using the Student's t-test or one-way ANOVA followed by Bonferroni's posttest, to multiple comparisons, and P values were calculated by comparison with control groups. ED50 (dose of a drug that produces 50% of its maximum effect) values were determined by nonlinear regression. All data were analyzed using GraphPad Prism (Software, Inc., San Diego, USA).
| Results and Discussion|| |
Given the occurrence of Croton species with toxic effects, for example, Croton leiophyllus,Croton argyratus, and C. tiglium. We investigated the toxicological potential using behavioral and pharmacological models in vivo of the CG-EtOH extract.
In the investigation of the cytotoxic effect of the extract (81, 243, and 500 mg/mL), we observed that it was unable to produce hemolysis in rat erythrocytes indicating that CG-EtOH had no hemolytic activity at the doses evaluated. In behavioral assessment and determination of toxicity, male mice (n = 6) showed no behavioral changes, both at doses of 2500 and 5000 mg/kg orally and doses of 1000 and 2000 mg/kg i.p., in the first 4 h of observation, nor was there any death during the 72 h following injection.
Similarly, in females (n = 6), the extract at doses of 2500 and 5000 mg/kg orally and 1000 mg/kg by i.p. did not cause behavioral changes or death. In contrast, the extract when given i.p. at a dose of 2000 mg/kg showed lethality in all animals evaluated (n = 6). Thus, in female mice was administered the extract at a dose of 1500 mg/kg i.p. Interestingly, they showed no changes or death at this dose.
These data suggest that the extract has low toxicity since only induced the death of the female at a dose of 2000 mg/kg (i.p.), possibly due to the action of hormones, present only in females, that induce changes in the metabolism or absorption of these metabolites, unlike in male mice.
These results indicate that CG-EtOH is not toxic with oral (p.o.) treatment, which is an interesting result since Resolution RE 90/2004 of the Agência Nacional de Vigilância Sanitária says it should be tested via the same route proposed for the use of the product. Conversely, i.p. administration of the extract in females but only at the highest dose used (2000 mg/kg), induced behavioral changes before death, including sedation, lethargy, and drowsiness. It is also possible that the lethal dose (i.p.) may be an intermediate value between 1500 and 2000 mg/kg or simply 2000 mg/kg. Although the extract has shown lethal effect at the dose of 2000 mg/kg, this dose is much higher than the doses used in this study later in anti diarrheal test, moreover, only i.p. the extract showed this effect.
C. urucurana Baill. (600 and 800 mg/kg) has been reported that significant antidiarrheal effect  and several Croton species are used in folk medicine as anti-diarrheal, e.g. C. cajucara,C. tiglium, and C. urucurana. Therefore, we decided to investigate antidiarrheal activity by castor oil-induced diarrhea model in mice, and we observed that CG-EtOH (62.5, 125, 250, and 500 mg/kg) significantly reduced the frequency of defecation in a dose-dependent manner when compared to the negative control (saline + Cremofor ®), with inhibition values of 34.7 ± 5.0, 44.4 ± 5.6, 81.9 ± 7.3, and 75.0% ±3.7%, respectively (ED50 = 106.0 ± 8.1 mg/kg) [Figure 1]a. Similarly, in relation to liquid stools, CG-EtOH inhibited diarrhea induced by castor oil in a significant and dose-dependent manner with values of 31.8 ± 5.0, 52.2 ± 4.6, 100.0 and 88.6 ± 4.2, respectively, (ED50 = 105.0 ± 9.2 mg/kg) [Figure 1]b. Interestingly, this effect was more potent than observed with C. urucurana.
|Figure 1: Antidiarrheal effect of the CH-EtOH extract in the castor oil-induced diarrhea model in mice (n = 6). (a) Percentage of defecation frequency and (b) percentage of liquid stool. Columns and vertical bars represent the percentage of the mean and standard error of the mean, respectively. One-way ANOVA followed by Bonferroni test, **P < 0.001 (saline vs. loperamide/extract)|
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The maximum effect of the extract in reducing both the frequency of defecation (Emax = 82.0% ±7.3%) and liquid stool (Emax = 100%) was observed at a dose of 250 mg/kg, similar to the standard drug, loperamide (Emax = 95.8 ± 1.8 and 100%, respectively). These results suggest that the extract has active metabolites with antidiarrheal activity, and these metabolites probably are in agreement with the species of this genus (flavonoids, terpenoids, and alcaloids),, since it is used in folk medicine to treat this illness. Furthermore, antidiarrheal activity has been demonstrated with some species this genus, as, e.g. C. cajucara,C. tiglium, and C. urucurana.
As diarrhea often appears with signs of increased motility and/or increased secretion of the gastrointestinal mucosa in response to various stimuli, we decided to evaluate the effect of CG-EtOH on motility and intestinal fluid in mice.
Similar to the atropine group, CG-EtOH only at doses of 125 and 250 mg/kg significantly reduced gastrointestinal transit by 65.1 ± 3.7 and 66.7% ±1.5%, respectively, compared to the control group. At doses of 62.5 and 500 mg/kg, the extract did not reduce gastrointestinal transit significantly [Figure 2]a. Based on these results, we suggest that the antidiarrheal effect of CG-EtOH involves changes in intestinal motility showing a maximum effect around 65%.
|Figure 2: Effect of CH-EtOH extract on the intestinal transit (a) and fluid accumulation induced by castor oil (b) in mice (n = 6). Columns and vertical bars represent the percentage of the mean and standard error of the mean, respectively. One-way ANOVA followed by Bonferroni, *P < 0.05 and **P < 0.001 (saline vs. loperamide/extract)|
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Oral administration of CG-EtOH (7.81, 15.62, 31.25, 62.5, 125, and 250 mg/kg) inhibited, in a significant and dose-dependent manner, the accumulation of intestinal fluid induced by castor oil, compared to the negative control. Inhibition values were 9.9 ± 3.3, 14.7 ± 3.9, 31.7 ± 7.2, 45.5 ± 6.2, 64.6 ± 2.4, 67.5% ±3.5%, respectively (ED50 = 34.6 ± 5.4 mg/kg) [Figure 2]b.
The maximum effect of CG-EtOH to inhibit the content of intestinal fluid (Emax = 67.5% ±3.5%) was observed at a dose of 250 mg/kg, similar to the standard drug, loperamide (Emax = 70.6% ±1.0%). The results showed that CG-EtOH has an inhibitory effect on the accumulation of intestinal fluid [Figure 2]b, suggesting that the antidiarrheal effect involves mainly changes in intestinal secretion. This action is the most desirable since the main manifestation common to different types of diarrhea is dehydration, and in many cases, the inhibition of intestinal transit is not desired, as it may delay or prevent the elimination of potential pathogens.
The results show that the CG-EtOH probably contains active metabolites with antidiarrheal activity since it had a significant antidiarrheal effect in mice with a reduction in the frequency and number of liquid stools, and that these metabolites act mainly by reducing intestinal fluid and to a lesser extent, reducing intestinal motility.
The authors thank PIBIC/UFAL/FAPEAL for financial support. Dr. A. Leyva helped with English editing of the manuscript.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Veiga-Junior VF, Pinto AC. Plantas medicinais: Cura segura? Quím Nova 2005;28:519-28.
Schoppan LT, Gastal CV. Plantas medicinais de um parque municipal de Porto Alegre, RS-Brasil, Caderno de Pesquisa. Sér Biol 2009;21:31-45.
Cronquist A. An Integrated System of Classification of Flowering Plants. New York: Columbia University Press; 1981. p. 1262.
Webster GL. Synposis of the genera suprageneric taxa of Euphorbiaceae
. Ann Mo Bot Gard 1994;81:144.
Braga R. Plantas do Nordeste: Especialmente do Ceará. Natal: Editora Universitária-RN; 1960. p. 540.
Giang PM, Son PT, Lee JJ, Otsuka H. Four ent-kaurane-type diterpenoids from Croton tonkinensis
Gagnep. Chem Pharm Bull (Tokyo) 2004;52:879-82.
Kuo PC, Shen YC, Yang ML, Wang SH, Thang TD, Dung NX, et al.
Crotonkinins A and B and related diterpenoids from Croton tonkinensis
as anti-inflammatory and antitumor agents. J Nat Prod 2007;70:1906-9.
Thuong PT, Dao TT, Pham TH, Nguyen PH, Le TV, Lee KY, et al.
Crotonkinensins A and B, diterpenoids from the Vietnamese medicinal plant Croton tonkinensis
. J Nat Prod 2009;72:2040-2.
Compagnone RS, Chavez K, Mateu E, Orsini G, Arvelo F, Suárez AI. Composition and cytotoxic activity of essential oils from Croton matourensis
and Croton micans
from Venezuela. Rec Nat Prod 2010;2:101-8.
Fattorusso E, Taglialatela-Scafati O, Campagnuolo C, Santelia FU, Appendino G, Spagliardi P. Diterpenoids from Cascarilla (Croton eluteria
Bennet). J Agric Food Chem 2002;50:5131-8.
Silva JS, Sales MF, Torres DS. O gênero Croto
e) na microrregião do Vale do Ipanema, Pernambuco, Brasil. Rodriguésia 2009;60:879-901.
Martinez CA, Barua D, Merson MH. Control of diarrhoeal diseases. World Health Stat Q 1988;41:74-81.
Maciel MA, Pinto AC, Arruda AC, Pamplona SG, Vanderlinde FA, Lapa AJ, et al.
Ethnopharmacology, phytochemistry and pharmacology: A successful combination in the study of Croton cajucara
. J Ethnopharmacol 2000;70:41-55.
Wang X, Zhang F, Liu Z, Feng H, Yu ZB, Lu Y, et al.
Effects of essential oil from Croton tiglium
L. on intestinal transit in mice. J Ethnopharmacol 2008;117:102-7.
Gurgel LA, Silva RM, Santos FA, Martins DT, Mattos PO, Rao VS. Studies on the antidiarrhoeal effect of dragon's blood from Croton urucurana
. Phytother Res 2001;15:319-22.
Santos CB, Araújo KC, Jardim-Botelho A, Santos MB, Rodrigues A, Dolabella SS, et al.
Diarrhea incidence and intestinal infections among rotavirus vaccinated infants from a poor area in Brazil: A spatial analysis. BMC Public Health 2014;14:399.
Guerrant RL, Schorling JB, McAuliffe JF, de Souza MA. Diarrhea as a cause and an effect of malnutrition: Diarrhea prevents catch-up growth and malnutrition increases diarrhea frequency and duration. Am J Trop Med Hyg 1992;47 (1 Pt 2):28-35.
Medeiros VM, Tavares JF, Almeida JR, Araújo Junior VT, Athayde-Filho PF, Cunha EV, e
l. Phytochemistry of the genus Croton. In: Gupta VK, editor. Natural Products: Research Reviews.1s
t ed., Vol. 1. New Delhi: Daya Publishing House; 2012. p. 221-370.
Medeiros VM. Estudo Fitoquímico de Croto
s Baill. e Revisão da Ocorrência das Principais Classes de Metabólitos do Gênero Croto
n. João Pessoa. Dissertação de Mestrado, Programa de Pós-Graduação em Produtos Naturais da Universidade Federal da Paraíba; 2012.
Rangel M, Malpezzi EL, Susini SM, de Freitas JC. Hemolytic activity in extracts of the diatom Nitzschia
. Toxicon 1997;35:305-9.
Awouters F, Niemegeers CJ, Lenaerts FM, Janssen PA. Delay of castor oil diarrhoea in rats: A new way to evaluate inhibitors of prostaglandin biosynthesis. J Pharm Pharmacol 1978;30:41-5.
Rao VS, Santos FA, Sobreira TT, Souza MF, Melo CL, Silveira ER. Investigations on the gastroprotective and antidiarrhoeal properties of ternatin, a tetramethoxyflavone from Egletes viscosa
. Planta Med 1997;63:146-9.
Di Carlo G, Autore G, Izzo AA, Maiolino P, Mascolo N, Viola P, et al.
Inhibition of intestinal motility and secretion by flavonoids in mice and rats: Structure-activity relationships. J Pharm Pharmacol 1993;45:1054-9.
Horgen FD, Edrada RA, de los Reyes G, Agcaoili F, Madulid DA, Wongpanich V, et al.
Biological screening of rain forest plot trees from Palawan Island (Philippines). Phytomedicine 2001;8:71-81.
Yadav RP, Singh A. Toxic effects of crotocaudin extracted from the medicinal plant Croton tiglium
. Z Naturforsch C 2010;65:327-36.
Poulsen HE, Loft S. The impact of genetic polymorphisms in risk assessment of drugs. Arch Toxicol Suppl 1994;16:211-22.
Gurgel LA, Martins DT, Mattos PO, Rao VS. Estudo da atividade antidiarréica e antissecretória intestinal do látex do Croto
a Baill. Rev Bras Farmacognosia 2002;12:39-42.
Berry PE, Hipp AL, Wurdack KJ, Van Ee B, Riina R. Molecular phylogenetics of the giant genus Croton
and tribe Crotoneae (Euphorbiaceae
sensu stricto) using ITS and TRNL-TRNF DNA sequence data. Am J Bot 2005;92:1520-34.
Salatino A, Salatino ML, Negri G. Traditional uses, chemistry and pharmacology of Croton
). J Braz Chem Soc 2007;18:11-3.
Estrada-Soto S, Rodríguez-Avilez A, Castañeda-Avila C, Castillo-España P, Navarrete-Vázquez G, Hernández L, et al.
Spasmolytic action of Lepechinia caulescens
is through calcium channel blockade and NO release. J Ethnopharmacol 2007;114:364-70.
| Authors|| |
Fabiana de Andrade Cavalcante, is an Adjunct Professor at the Pharmacy and Medicine Course, Federal University of Paraνba (UFPB, Joγo Pessoa). She is professor of the Postgraduate Program in Natural Products and Bioactive Synthetic (UFPB, Joγo Pessoa). She has projects in collaboration with national institutions. Has experience in the area of Pharmacology and Physiology of Natural Products.
[Figure 1], [Figure 2]