Home | About PR | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |   Login 
Pharmacognosy Magazine
Search Article 
Advanced search 
Year : 2013  |  Volume : 5  |  Issue : 2  |  Page : 86-92

Regeneration of multiple shoots from petiole callus of Viola serpens Wall

Department of Botany, National Research Institute of Basic Ayurvedic Sciences, Kothrud, Pune, MH, India

Correspondence Address:
Arun Manohar Gurav
National Research Institute of Basic Ayurvedic Sciences, Kothrud, Pune, MH
Login to access the Email id

Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0974-8490.110533

Rights and Permissions

Experiments were conducted to develop methodology for in vitro propagation and rapid multiplication of Viola serpens Wall. using petiole explant. The MS medium supplemented with 2, 4-D (6.78 μm) was found most suitable for callus induction in petiole explant. The best growth response and higher rate of shoot regeneration from petiole callus was observed on MS medium containing BAP (11.10 μm) as the average number of shoots could be increased to 36.4 on fourth successive subculturing. Higher rooting response with larger number of roots were observed in shoots inoculated on the half-strength MS medium supplemented with IBA (19.68 μm).

Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded56    
    Comments [Add]    
    Cited by others 1    

Recommend this journal