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Year : 2013  |  Volume : 5  |  Issue : 2  |  Page : 65-70

Insulin secretion enhancing activity of roselle calyx extract in normal and streptozotocin-induced diabetic rats

1 Department of Pharmacology, Khon Kaen University, Khon Kaen, Thailand
2 Department of Physiology, Khon Kaen University, Khon Kaen, Thailand
3 Department of Pathology, Khon Kaen University, Khon Kaen, Thailand
4 Applied Thai Traditional Medicine Center, Thammasat University, Prathumthani, Thailand

Correspondence Address:
Patchareewan Pannangpetch
Department of Pharmacology, Khon Kaen University, Khon Kaen
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Source of Support: National Research Council of Thailand and Khon Kaen University Graduate Research Fund, Khon Kaen University, Thailand,, Conflict of Interest: None

DOI: 10.4103/0974-8490.110520

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Background and Objective: Our recent study revealed the antihyperglycemic activity of an ethanolic extract of roselle calyxes (Hibiscus sabdariffa) in diabetic rats. The present study had, therefore, an objective to investigate the mechanism underlying this activity. Materials and Methods: Male Sprague Dawley rats were induced to be diabetes by intraperitoneal injection of 45 mg/kg streptozotocin (STZ). Normal rats as well as diabetic rats were administered with the ethanolic extract of H. sabdariffa calyxes (HS-EE) at 0.1 and 1.0 g/kg/day, respectively, for 6 weeks. Then, blood glucose and insulin levels, at basal and glucose-stimulated secretions, were measured. The pancreas was dissected to examine histologically. Results: HS-EE 1.0 g/kg/day significantly decreased the blood glucose level by 38 ± 12% in diabetic rats but not in normal rats. In normal rats, treatment with 1.0 g/kg HS-EE increased the basal insulin level significantly as compared with control normal rats (1.28 ± 0.25 and 0.55 ± 0.05 ng/ml, respectively). Interestingly, diabetic rats treated with 1.0 g/kg HS-EE also showed a significant increase in basal insulin level as compared with the control diabetic rats (0.30 ± 0.05 and 0.15 ± 0.01 ng/ml, respectively). Concerning microscopic histological examination, HS-EE 1.0 g/kg significantly increased the number of islets of Langerhans in both normal rats (1.2 ± 0.1 and 2.0 ± 0.1 islet number/10 low-power fields (LPF) for control and HS-EE treated group, respectively) and diabetic rats (1.0 ± 0.3 and 3.9 ± 0.6 islet number/10 LPF for control and HS-EE treated group, respectively). Conclusion: The antidiabetic activity of HS-EE may be partially mediated via the stimulating effect on insulin secretion.

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