| ORIGINAL ARTICLE |
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| Year : 2012 | Volume
: 4
| Issue : 4 | Page : 189-195 |
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Modulation of genotoxicity of oxidative mutagens by glycyrrhizic acid from Glycyrrhiza glabra L.
Prabhjit Kaur1, Neha Sharma1, Bikram Singh2, Subodh Kumar3, Satwinderjeet Kaur1
1 Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar, India
2 Division of Natural Plant Product, Institute of Himalayan Bioresource Technology, Palampur, HP, India
3 Department of Chemistry, Guru Nanak Dev University, Amritsar, India
Correspondence Address:
Satwinderjeet Kaur
Department of Botanical and Environmental Sciences, Guru Nanak Dev University, Amritsar - 143005, Punjab
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0974-8490.102260
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Background: The chemopreventive effects of certain phytoconstituents can be exploited for their use as functional foods, dietary supplements and even as drugs. The natural compounds, acting as anti-genotoxic and free radical scavenging compounds, may serve as potent chemopreventive agents. These can inhibit DNA modulatory activities of mutagens and help preventing pathological processes. Objectives: Present study on Glycyrrhiza glabra L., a promising medicinal plant, widely used in traditional medicine, focused on the bioassay-guided fractionation of its extracts for the isolation of certain phytochemicals with anti-genotoxic potential against oxidative mutagens. Materials and Methods: The methanol extract of Glycyrrhiza glabra rhizomes was subjected to column chromatography, and isolated fraction was evaluated for its anti-genotoxic and antioxidant potential using SOS chromotest, Comet assay, and DPPH radical scavenging assay. Results: GLG fraction, which was characterized as Glycyrrhizic acid, inhibited the genotoxicity of oxidative mutagens viz., H 2 O 2 and 4NQOquite efficiently. In SOS chromotest, using E.coli PQ37 tester strain, it inhibited induction factor induced by H 2 O 2 and 4NQO by 75.54% and 71.69% at the concentration of 121.46 μM,respectively. In Comet assay, it reduced the tail moment induced by H 2 O 2 and 4NQO by 70.21% and 69.04%, respectively, at the same concentration in human blood lymphocytes. The isolated fraction also exhibited DPPH free radical scavenging activity and was able to scavenge 85.95% radicals at a concentration of 120 μM. Conclusion: Glycyrrhizic acid is a potential modulator of genotoxins as well as efficient scavenger of free radicals. |
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