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Year : 2010  |  Volume : 2  |  Issue : 3  |  Page : 195-201 Table of Contents     

Antihyperglycemic activity of Catharanthus roseus leaf powder in streptozotocin-induced diabetic rats

1 Department of Biochemistry, Sri Krishnadevaraya University, Anantapur-515 003, Andhra Pradesh, India
2 Department of Biochemistry, Sri Venkateswara University, Tirupati-517502, Andhra Pradesh, India

Date of Submission06-Jan-2010
Date of Decision20-Feb-2010
Date of Web Publication19-Jul-2010

Correspondence Address:
Saralakumari Desireddy
Department of Biochemistry, Sri Krishnadevaraya University, Anantapur - 515 003, Andhra Pradesh
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0974-8490.65523

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Catharanthus roseus Linn (Apocynaceae), is a traditional medicinal plant used to control diabetes, in various regions of the world. In this study we evaluated the possible antidiabetic and hypolipidemic effect of C. roseus (Catharanthus roseus) leaf powder in diabetic rats. Diabetes was induced by intraperitoneal injection of streptozotocin (STZ, 55 mg/kg body wt) to male Wistar rats. The animals were divided into four groups: Control, control-treated, diabetic, and diabetic-treated group. Diabetic-treated and control-treated rats were treated with C. roseus leaf powder suspension in 2 ml distilled water, orally (100 mg/kg body weight/day/60 days). In diabetic rats (D-group) the plasma glucose was increased and the plasma insulin was decreased gradually. In the diabetic-treated group lowering of plasma glucose and an increase in plasma insulin were observed after 15 days and by the end of the experimental period the plasma glucose had almost reached the normal level, but insulin had not. The significant enhancement in plasma total cholesterol, triglycerides, LDL and VLDL-cholesterol, and the atherogenic index of diabetic rats were normalized in diabetic-treated rats. Decreased hepatic and muscle glycogen content and alterations in the activities of enzymes of glucose metabolism (glycogen phosphorylase, hexokinase, phosphofructokinase, pyruvate kinase, and glucose-6-phosphate dehydrogenase), as observed in the diabetic control rats, were prevented with C. roseus administration. Our results demonstrated that C. roseus with its antidiabetic and hypolipidemic properties could be a potential herbal medicine in treating diabetes.

Keywords: Anti Catharanthus roseus, plasma insulin, plasma lipids, STZ-induced diabetes

How to cite this article:
Rasineni K, Bellamkonda R, Singareddy SR, Desireddy S. Antihyperglycemic activity of Catharanthus roseus leaf powder in streptozotocin-induced diabetic rats. Phcog Res 2010;2:195-201

How to cite this URL:
Rasineni K, Bellamkonda R, Singareddy SR, Desireddy S. Antihyperglycemic activity of Catharanthus roseus leaf powder in streptozotocin-induced diabetic rats. Phcog Res [serial online] 2010 [cited 2021 Mar 9];2:195-201. Available from: http://www.phcogres.com/text.asp?2010/2/3/195/65523

   Introduction Top

Diabetes mellitus is a metabolic disorder characterized by hyperglycemia and disturbance of carbohydrate, protein, and fat metabolism along with long-term complications affecting the retina, kidney, and nervous system. [1] Different types of oral hypoglycemic agents such as biguanides and sulfonylurea are available along with insulin for the treatment of diabetes. Unfortunately, apart from having a number of side effects, none of the oral synthetic hypoglycemic agents have been successful in maintaining euglycemia or controlling long-term microvascular and macrovascular complications. Although insulin therapy is also used for management of diabetes mellitus, there are several drawbacks, such as, insulin resistance, anorexia nervosa, brain atrophy, and fatty liver after chronic treatment. [2] There is a growing interest in herbal remedies, because of their effectiveness, minimal side effects in clinical experience, and relatively low cost. Herbal drugs or their extracts are prescribed widely; even their biologically active compounds are unknown. [3] In many developing countries, traditional medicine, in particular the herbal medicine, is sometimes the only affordable source of healthcare. [4] Even the WHO (World Health Organization) approves the use of plant drugs for different diseases, including diabetes mellitus. [5] Therefore, studies with plant extracts are useful, to know their efficacy and mechanism of action and safety.

Catharanthus roseus L (Apocynaceae) is a subshrub also known as Medagaskar periwinkle, Vinca rosea or Lanchnera rosea worldwide. The plant Catharanthus roseus (C. roseus) has gained acceptance from the pharmaceutical industries, as it is widely used as an infusion in different parts of world, to treat diabetes. [6],[7] The fresh juice from the flowers of C. roseus, made into a tea, has been used by Ayurvedic physicians in India for external use to treat skin problems, dermatitis, eczema, and acne. The ethanol extract of the C. roseus flower has been reported to have wound healing activity. [8] The sulfates of C. roseus alkaloids, vincristine and vinblastine, are widely used as chemotherapeutic agents against Leukemia and Hodgkin's disease worldwide. [9] Hot water decoction of the leaves and / or the whole plant is used for the treatment of diabetes in several countries. [10] Significant antihyperglycemic activities of the leaf alcoholic extract, [11],[12] aqueous extract, [13] and the dichloromethane-methanol extract of leaves and twinges [14] have been reported in laboratory animals. Fresh leaf juice of C. roseus has been reported to reduce blood glucose in normal and alloxan diabetic rabbits. [15] Although, earlier reports indicate blood glucose lowering activity in alcoholic extracts of leaves, studies regarding C. roseus leaf powder efficacy in the management of hyperglycemia have not been undertaken. In the present study we evaluated the antidiabetic and hypolipidemic activity of C. roseus leaf powder suspension in STZ-induced diabetic rats.

   Materials and Methods Top


Streptozotocin, lactate dehydrogenase, and glucose-6-phosphate dehydrogenase were obtained from the Sigma Chemical Co., St. Louis, MO, USA. All other chemicals were of analytical grade and procured from Sisco Research Laboratories (P) Ltd., Mumbai, India.

Plant material

Catharanthus roseus (white variety) was taxonomically authenticated by the Department of Botany, Sri Krishnadevaraya University, Anantapur, and the voucher specimen was kept in the herbarium (No. 2235) of our University. Fresh leaves of C. roseus (white variety) were collected during September, from the University campus, and the leaves were shade dried and then grinded to fine powder.

Induction of diabetes to experimental animals

Two-to-three-month-old male albino Wistar rats of body weight 150 - 180 g were procured from Sri Venkateswara Enterprises (Bangalore, India), acclimatized for seven days to our animal house, and maintained at standard conditions of temperature and relative humidity, with a 12-hour light / dark cycle. Water and commercial rat feed were provided ad libitum. The current study was carried out with prior permission from our Institutional Animal Ethical Committee (Regd. no. 470/01/a/CPCSEA, dt. 24 August, 2001). Diabetes was induced in overnight fasted rats by a single intraperitoneal injection of freshly prepared STZ (55 mg/kg body weight, in ice-cold 0.1 M citrate buffer, pH 4.5, in a volume of 0.1 ml per rat). Seventy-two hours after STZ administration, the plasma glucose level of each rat was determined, for confirmation of diabetes. Rats with plasma glucose level above 250 mg/dl were considered as diabetics and used subsequently.

Experimental design and biochemical analysis

In the present experiment, a total of 32 rats (16 diabetic surviving rats; 16 normal rats) were used. The rats were divided into four groups of eight each: control (C); control rats treated with C. roseus (C + CR); diabetic (D), and diabetic animals treated with C. roseus (D + CR). Diabetic-treated group and C + CR-group received C. roseus leaf powder suspension (100 mg/kg body weight) in 2 ml distilled water daily, for 60 days, through oral intubation, whereas, 2 ml of distilled water was administered to D + CR and C + CR rats. Based on the preliminary experiments on the dose-dependent antihyperglycemic effect of leaf powder, a dose less than 100 mg/kg body weight was not found to be effective in rats. Body weight was monitored at 15-day intervals. During the experimental period, blood was collected from 12-hour fasted rats by means of a capillary tube through the orbital sinus, at 15-day intervals. Plasma glucose was estimated by the glucose oxidase-peroxidase (GOD-POD) method, by using the Span diagnostic kit (Span diagnostic Ltd., Surath, India). Triglycerides, total cholesterol, and HDL-cholesterol were measured by enzymatic colorimetric end point methods using the Span diagnostic reagent kit. LDL and VLDL-cholesterol were obtained by calculations using the formula provided in the cholesterol diagnostic kit booklet. Plasma insulin was estimated by using the radioimmunoassay kit (RIA K-1) from the Bhabha Atomic Research Center (Mumbai, India).

Animal sacrifice

Animals from each experimental group were starved for 16 hours and sacrificed by cervical dislocation. The liver, muscle, and kidneys were removed, washed thoroughly with ice-cold saline and used for analysis.

Glycogen content

Glycogen released from a protein-free supernatant of trichloroacetic acid (TCA)-homogenized tissues were precipitated with 95% ethanol. The precipitated glycogen was then hydrolyzed under acidic condition and the liberated glucose was estimated by the Anthrone method as adapted by Carrol et al. [16]

Assay of carbohydrate metabolism enzymes

Liver and muscle homogenates were prepared in 0.1M Tris-HCl buffer, pH 7.4, and used for assay of hexokinase (HK), [17] phosphofructokinase PFK, [18] and pyruvate kinase (PK), [18] and glucose-6-phosphate dehydrogenase (G6PDH) [19] and glycogen phospharylase, [20] were assayed.

Statistical analysis

The results were expressed as means ± S.E. Data were analyzed for significant differences using Duncan's Multiple Range (DMR) test. (P < 0.05).

   Results Top

Body weight

Characteristic symptoms of diabetes such as loss of body weight, polyphagia, polydipsia, and polyuria observed in the D-group, were rectified in the D + CR-group. At the end of the 60-day experimental period, the D-group showed 21.9% reduction in body weight, whereas, the C, C + CR, and D + CR-groups showed an increase in body weight of 51.4, 53, and 20.2%, respectively [Figure 1]. However, by the end of the experimental period the D + CR-group showed a significant increase (52.8%) in body weight when compared to the D-group.

Plasma glucose and insulin

Data on the plasma glucose content of the four experimental groups are presented in [Figure 2]. Group-C and C + CR-rats remained persistently euglycemic throughout the experimental period. In the D-group, the plasma glucose level gradually increased during the experimental period from 390.87 ± 1.18 to 420.25 ± 2.8 mg/dl. A significant antihyperglycemic effect was evident in the D + CR-group from 15 days onwards and the decrease in plasma glucose was 77.7% by 60 days of treatment.

The fasting plasma insulin levels of the four groups of animals during the experimental period are presented in [Figure 3]. The Control-treated group showed statistically lower insulin levels at 45 and 60 days when compared with the C-group. By the end of the experimental period, D-group plasma insulin was decreased from 11.2 ± 0.18 to 9.33 ± 0.2 μU/ml. Group-D + CR showed significantly higher concentration of insulin at 15 days (12.39 ± 0.5), 30 days (18.70 ± 0.6), 45 days (18.62 ± 0.6), and 60 days (19.87 ± 0.8) with 13.7, 82, 87.7, and 109.1% increase, respectively, when compared with D-group. At the end of experimental period the insulin level of the D + CR-group was significantly higher than the D-group, but still significantly lower than the C-group.

Plasma lipid profile

The plasma lipid profiles of the four groups of animals during the experimental period are represented in [Table 1]. A significant increase in the plasma total cholesterol (13.2%), triglycerides (17.2%), LDL-cholesterol (37.4%), and VLDL-cholesterol (17.2%), and a significant decrease in HDL-cholesterol (11.31%) in the D-group compared to the C-group, resulted in a significant increase in the atherogenic index (27.9%). A significant decrease in plasma total cholesterol (14.6%), triglycerides (9.8%), LDL-cholesterol (61.8%), VLDL-cholesterol (9.84%), and a significant increase in the HDL-cholesterol concentration (28.3%) in the C + CR-group compared to the C-group resulted in a significant decrease in atherogenic index (33.5%). Group D + CR showed a significant decrease in plasma total cholesterol (8.4%), triglycerides (13.3%), LDL-cholesterol (28.7%), VLDL-cholesterol (13.3%), and the atherogenic index (28.7%), and a significant increase in HDL-cholesterol concentration (24.5%) when compared with the D-group [Table 1].


Group-D showed significantly decreased glycogen content in the liver (60.9%) and muscle (70.3%), when compared to the C-group. In the D + CR-group C. roseus treatment resulted in a significant enhancement in the liver (94.8%) and muscle (104%) glycogen content when compared to the D-group [Table 2].

Carbohydrate metabolism enzymes

[Table 2] shows activities of carbohydrate metabolic enzymes in the liver and muscle. The STZ diabetic rats (D-group) showed a significantly enhanced hepatic glycogen phosphorylase activity (119.4%) compared to the C-group. The D + CR rats showed decreased activity (48.3%) of glycogen phosphorylase compared to the D-group rats. The STZ-induced diabetic rats showed significantly decreased activities of glycolytic enzymes, both in the liver and muscles, compared to the control rats. The percent decrease in HK, PFK, and PK activities in the liver and muscle of the D-group are 43.2, 37.3, and 32.2%, and 40.7, 58.1, and 37.6%, respectively. C. roseus treated diabetic rats, that is, the D + CR-group showed no deviation in the activities of HK, PFK, and PK, both in the liver and muscle compared to the C-group. Thus C. roseus treatment in the D + CR-group prevented diabetic-induced alterations in the glycolytic enzyme activities. Hepatic G6PDH activity decreased significantly (54.3%) in the D-group. C. roseus treatment resulted in a significant enhancement in the activity of G6PDH in the liver of the C + CR and D + CR-groups (7.0 and 85.5%) compared to the C and D-groups, respectively.

   Discussion Top

As expected, STZ-induced D-group rats showed characteristic signs of diabetes such as, polyphagia, polydipsia, and polyuria, failure to gain in body weight, hyperglycemia, hypoinsulinemia, and hyperlipidemia. Inspite of polyphagia, a decrease in the body weight of diabetic rats was possible due to excessive catabolism of fats and protein. [21] In the D + CR-group, ingestion of C. roseus leaf powder effectively prevented these diabetic symptoms, indicating the antidiabetic nature of this plant. No visible side effects of C. roseus leaf powder were observed in the C + CR-group, representing the non-toxic nature of C. roseus. The plasma glucose levels observed in the C + CR and D + CR-groups during the experimental period clearly indicate that C. roseus leaf powder does not promote hypoglycemic activity, but exerts an antihyperglycemic effect. Chronic treatment of diabetic rats for a 60-day period with C. roseus leaf powder lowered the plasma glucose level to near normal levels. The gradual decrease in the plasma insulin levels of the C + CR-rats during the experimental period resulted in significantly lower values at 45 and 60 days compared to C-rats in the corresponding period. This result with the maintained normoglycemia [Figure 2] in C + CR-rats indicates that C. roseus promotes glucose uptake by promoting insulin sensitivity. In the STZ-induced diabetic model, insulin is markedly depleted, but not absent. [22] The hypoinsulinemia observed in STZ-induced diabetic rats is gradually intensified during the experimental period. A significant increase in the plasma insulin levels of the D + CR-group compared to the D-group may be due to the regeneration of the STZ-destructed β-cells, which is probably due to the fact that the pancreas contains stable (quiescent) cells that have the capacity to regenerate. [23],[24] Therefore, the surviving cells can proliferate to replace the lost cells. Phytochemicals such as flavonoids and alkaloids present in the C. roseus leaf powder [25] may have protected the intact functional β-cells from further deterioration through oxidative stress. Hence, the β-cells remain active and continue to produce insulin. It is also claimed that antioxidants such as flavonoids are possibly beneficial in preventing STZ-induced diabetes by stopping oxidative damage of the pancreas, and increasing insulin secretion by the regeneration of pancreatic β-cells.[26],[27]

In this study, we have noticed elevated levels of plasma lipids such as total cholesterol, LDL and VLDL-cholesterol, and triglycerides and decreased level of HDL-cholesterol in the D-group, which are risk factors for coronary heart disease. [28] Insulin increases uptake of fatty acids into the adipose tissue and increases triglyceride synthesis. [29] Moreover, insulin inhibits lipolysis. Lipolysis is not inhibited in the D-group due to the presence of insulin deficiency, leading to hyperlipidemia. It is interesting that treatment with C. roseus leaf powder suspension for 60 days brings down the elevated levels of total cholesterol, LDL and VLDL-cholesterol, and triglycerides, and also increases plasma HDL-cholesterol to normal levels in D + CR-rats, indicating the beneficial effect of C. roseus in reducing the risk of cardiovascular diseases. Increased levels of HDL-cholesterol, an antiatherogenic lipoprotein, after C. roseus administration may be due to an increase in the activity of lecithin cholesterol acyl transferase, which may contribute to the regulation of blood lipids. [30]

The liver plays an important role in buffering postprandial hyperglycemia and is involved in the synthesis of glycogen. Diabetes mellitus is known to impair the normal capacity of the liver to synthesize glycogen. [31] Assessment of glycogen levels serve as a marker for studying the antidiabetic activity of C. roseus. In the D-group, the glycogen content in the liver and muscle was reduced compared to control rats. This is in line with previous studies on STZ-diabetic rats. [32] Two months of treatment with C. roseus partially prevented the depletion of glycogen in the liver and muscle of STZ-diabetic rats. This could be due to the increased circulatory insulin concentrations observed in the D + CR-group compared to the D-group. Increased activity of hepatic glycogen phosphorylase observed in STZ-diabetic rats might be one contributing factor for the decreased hepatic glycogen content. The elevated hepatic glycogen content and decreased plasma glucose levels of C. roseus-treated, STZ-diabetic rats compared to STZ-diabetic control rats could be explained by the diminished activity of the glycogenolytic enzyme, namely, glycogen phosphorylase.

Diabetes mellitus is characterized by partial or total deficiency of insulin, resulting in derangement of carbohydrate metabolism and a decrease in the enzymatic activity of HK and PFK, resulting in the depletion of liver and muscle glycogen. [33] As insulin administration normalizes these alterations in the enzymatic activities, these enzymes represent a method to assess the peripheral utilization of glucose. Glycolytic enzymes (HK, PFK, PK) activity of the D-group was significantly decreased in the liver and muscle, and this was similar to the previous findings. [34],[35] An increase in the activity of glycolytic enzymes in C. roseus-treated diabetic rats implied that the cellular entry of glucose was facilitated by C. roseus, which in turn stimulated the activity of these enzymes. This glucose influx is due to an increase in the circulation of insulin and insulin sensitivity. Glucose-6-phosphate dehydrogenase is the key regulating enzyme of the pentose phosphate pathway and controls the flow of carbon through this pathway. Specifically, the enzyme catalyzes the first reaction in the pathway leading to the production of pentose phosphates and reduces power in the form of Nicotinamide Adenosine Dinucleotide Phosphate (NADPH) for reductive biosynthesis and maintenance of the redox state of the cell. Alterations in G6PDH activity can significantly alter oxidative stress-induced cell death. [36] C. roseus administration increases the G6PDH activity in D + CR- rats. Insulin is reported to stimulate oxidation of glucose by increasing the activation of G6PDH in a dose-dependent manner. [37] Thus, the increased circulatory insulin level observed in C. roseus-treated STZ-diabetic rats may cause increased activity of G6PDH in these rats.

Based on our observations of carbohydrate metabolism, the antihyperglycemic effect of this plant appears to be at least in part, due to extra pancreatic activity, including increased glucose utilization by the liver and muscle (glycolysis), enhanced glucose oxidation through shunt pathway, via activation of G6PDH, and decreased glucose production by depression of glycogenolytic enzyme.

   Conclusions Top

Thus our findings show that oral administration of C. roseus leaf powder produces an antihyperglycemic effect, lowers both total cholesterol and triglyceride levels, and at the same time increases HDL-cholesterol in STZ-induced diabetic rats. The antihyperglycemic action of the leaf powder of C. roseus is associated with increased plasma insulin concentration and insulin sensitivity. This investigation shows the potential of C. roseus, for use as a natural oral agent, with both antihyperglycemic and hypolipidemic effects. Further comprehensive biochemical and pharmacological investigations are needed to elucidate the exact mechanism of the antihyperglycemic effect of C. roseus.

   Acknowledgments Top

We appreciate the National Center for Laboratory Animal Sciences, Hyderabad, for the kind supply of feed for the experimental animals. Thanks are also due to Prof. Ch. Appa Rao, K. Ramesh Babu, and Sri Venkateswara University, Tirupati, for their help in the insulin assay.

   References Top

1.Alberti KG, Zimmet PZ. New diagnostic criteria and classification of diabetes-again. Diabet Med 1998;15:535-6.   Back to cited text no. 1      
2.Piedrola G, Novo E, Escobar F, Garcia-Robles R. White blood cell count and insulin resistance in patients with coronary artery disease. Ann Endocrinol (Paris) 2001;62:7-10.  Back to cited text no. 2      
3.Valiathan MS. Healing plants. Curr Sci 1998;75:112-6.  Back to cited text no. 3      
4.Hamdan II, Afifi FU. Studies on the in vitro and in vivo hypoglycemic activities of some medicinal plants used in treatment of diabetes in Jordanian traditional medicine. J Ethnopharmacol 2004;93:117-21.  Back to cited text no. 4      
5.WHO. Traditional Medicine Strategy 2002-2005. WHO Publications; 2002. p. 1-6.  Back to cited text no. 5      
6.Alxeandrova R, Alxeandrova I, Velcheva M, Varadino T. Phytoproducts and cancer. Exp Patho Parasitol 2000;4:15-25.  Back to cited text no. 6      
7.Heijden R.V, Jacob D.I, Soneijer W., Hallard D., Verpoorte R. The Catharanthous alkaloids: Pharmacognosy and biotechnology. Curr Med Chem 2004;11:607-28.   Back to cited text no. 7      
8.Nayak BS, Pinto Pereira LM. Catharanthus roseus flower extract has wound-healing activity in Sprague Dawley rats. BMC Complement Altern Med 2006;6:41.  Back to cited text no. 8      
9.Ozgen U, Turkoz Y, Stout M, Ozugurlu F, Pelik F, Bulut Y, et al . Degradation of vincristine by myeloperoxidase and hypochlorous acid in children with acute lymphoblastic leukemia. Leuk Res 2003;27:1109-13.   Back to cited text no. 9      
10.Don G. Catharanthus roseus In: Ross IA, editor. Medicinal Plants of the World. Totowa: Human Press; 1999. p. 109-18.  Back to cited text no. 10      
11.Chattopadhyay RR. A comparative evaluation of some blood glucose lowering agents of plant origin. J Ethanopharmacol 1999;67:367-6.  Back to cited text no. 11      
12.Mostofa M, Choudhury ME, Hossain MA, Islam MZ, Islam MS, Sumon MH. Antidiabetic effects of Catharanthus roseus, Azadirachta indica, Allium sativum and glimepride in experimentally diabetic induced rat. Bang J Vet Med 2007;5:99-102.   Back to cited text no. 12      
13.Islam A, Akhtar AM, Khan MR, Hossain MS, Alam MK, Wahed MI, et al. Antidiabetic and hypolipidemic effects of different fractions of Catharanthus roseus (Linn.) on normal and Streptozotocin-induced diabetic Rats. J Sci Res 2009;1:334-44.   Back to cited text no. 13      
14.Somananth S, Praveen V, Shoba S, Radhey S, Kumari MM, Ranganathan S, et al. Effect of an antidiabetic extract of a Catharanthus roseus on enzymatic activities in streptozotocin induced diabetic rats. J Ethanopharmacol 2001;76:269-77.  Back to cited text no. 14      
15.Nammi S, Boini MK, Lodagala SD, Behara RB. The fresh leaves of Catharanthus roseus Linn. Reduces blood glucose in normal and alloxan diabetic rabbits. BMC Complement Altern Med 2003;3:4.   Back to cited text no. 15      
16.Carrol NV, Longley RW, Roe JH. The determination of glycogen in liver and muscle by use of anthrone reagent. J Biol Chem 1956;220:583-7.  Back to cited text no. 16      
17.Easterby JS, Qadri SS. Hexokinase Type II from rat skeletal muscle. In: Colowick SP, Kaplan, editors. Methods Enzymol. Vol. 90. New York: Academic Press; 1973. p. 11-5.  Back to cited text no. 17      
18.Sadava D, Alonso D, Hong H, Pettit-Barrett DP. Effect of methadone addiction on glucose metabolism in rats. Gen Pharmacol 1997;28:27-9.  Back to cited text no. 18      
19.Beutler E. Red cell metabolism. A Manual of Biochemical methods. 2 nd ed. New York: Grune and Stratton; 1975. p. 66.  Back to cited text no. 19      
20.Sutherland EW. In: Colowick SP, Kaplan, editors. Methods in Enzymol 2. Academic Press; New York: 1955. p. 215-8.  Back to cited text no. 20      
21.Vats V, Yadav SP, Grover JK. Ethanolic extract of Ocimum sanctum leaves partially attenuated streptozotocin-induced alterations in glycogen content and carbohydrate metabolism in rats. J Ethnopharmacol 2004;90:155-60.  Back to cited text no. 21      
22.Pushparaj PN, Tan BK, Tan CH. The mechanism of hypoglycemic action of the semi purified fractions of Averrhoa bilimb in streptozotocin diabetic rats. Life Sci 2001;70:535-47.   Back to cited text no. 22      
23.Banerjee M, Kanitkar M, Bhonde RR. Approaches towards endogenous pancreatic regeneration. Rev Diabet Stud 2005;2:165-76.   Back to cited text no. 23      
24.Cano DA, Rulifson IC, Heiser PW, Swigart LB, Pelengaris S, German M, et al. Regulated β-cell regeneration in the adult mouse pancreas. Diabetes 2008;57:958-66.  Back to cited text no. 24      
25.Mustafa NR, Verpoorte R. Phenolic compounds in Catharanthus roseus. Phytochem Rev 2007;6:243-58.   Back to cited text no. 25      
26.Kamalakkannan N, Prince PS. Antihyperglycaemic and antioxidant effect of rutin, a polyphenolic flavonoid, in streptozotocin-Induced diabetic Wistar rats. Basic Clin Pharmacol Toxicol 2006;98:97-103.   Back to cited text no. 26      
27.Kishore A, Nampurath GK, Mathew SP, Zachariah RT, Potu BK, Rao MS, et al. Antidiabetic effect through islet cell protection in streptozotocin diabetes: A preliminary assessment of two thiazolidin-4-ones in Swiss albino mice. Chem Biol Interact 2009;177:242-6.  Back to cited text no. 27      
28.Stamler J, Wentworth D, Neaton JD. Is relationship between serum cholesterol and risk of premature death from coronary heart disease continuous and graded? Findings in 356 primary screens of the multiple risk factor intervention trial (MR-FIT). JAMA 1986;256:2823-8.   Back to cited text no. 28      
29.Shirwaikar A, Rajendran K, Dinesh Kumar C, Bodla R. Antidiabetic activity of aqueous extract of Annona squamosa in streptozotocin-nicotinamide type 2 diabetic rats. J Ethnopharmacol 2004;91:171-5.   Back to cited text no. 29      
30.Patil UK, Saraf S, Dixit VK. Hypolipidemic activity of seeds of Cassia tora Linn. J Ethnopharmacol 2004;90:249-52.   Back to cited text no. 30      
31.Whitton PD, Hems DA. Glycogen synthesis in the perfused liver of streptozotocin diabetic rats. Biochem J 1975;150:153-65.  Back to cited text no. 31      
32.Vats V, Yadav SP, Grover JK. Effect of Trigonella foenumgraceum on glycogen content of tissues and the key enzymes of carbohydrate metabolism. J Ethnopharmacol 2003;85:237-42.  Back to cited text no. 32      
33.Murphy ED, Anderson JW. Tissue glycolytic and gluconeogenic enzyme activities in mildly and moderately diabetic rats. influence of tobutamide administration. Endocrinology 1974;94:27-34.   Back to cited text no. 33      
34.Grover JK, Vats V, Rathi SS. Anti-hyperglycemic effects of Eugenia jambolana and Tinospora cordifolia in experimental diabetes and their effects on key metabolic enzymes involved in carbohydrate metabolism. J Ethnopharmacol 2000;73:461-70.  Back to cited text no. 34      
35.Rathi SS, Grover JK, Vats V. Anti-hyperglycemic effects of Momordica charantia and Mucuna pruriens in experimental diabetes and their effect on key metabolic enzymes involved in carbohydrate metabolism. Phytother Res 2002;16:236-43.   Back to cited text no. 35      
36.Vulliamy T, Mason P, Luzzatto L. The molecular basis of glucose-6-phosphate dehydrogenase deficiency. Trends Genet 1992;8:138-43.  Back to cited text no. 36      
37.Wagle A, Jivraj S, Garlock GL, Stapleton SR. Insulin regulation of glucose-6-phosphate dehydrogenase gene expression is rapamycin-sensitive and requires Phosphatidylinositol 3-Kinase. J Biol Chem 1998;273:14968-74.  Back to cited text no. 37      


  [Figure 1], [Figure 2], [Figure 3]

  [Table 1], [Table 2]

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Evidence-Based Complementary and Alternative Medicine. 2015; 2015: 1
[Pubmed] | [DOI]
7 Traditional management of diabetes in Pakistan: Ethnobotanical investigation from Traditional Health Practitioners
Ghulam Yaseen,Mushtaq Ahmad,Muhammad Zafar,Shazia Sultana,Sadaf Kayani,Adolfo Andrade Cetto,Shabnum Shaheen
Journal of Ethnopharmacology. 2015; 174: 91
[Pubmed] | [DOI]
8 Traditional plants used for the treatment of diabetes mellitus in Sursagar constituency, Jodhpur, Rajasthan – An ethnomedicinal survey
Manoj Goyal
Journal of Ethnopharmacology. 2015; 174: 364
[Pubmed] | [DOI]
9 Phytochemica: a platform to explore phytochemicals of medicinal plants
Shivalika Pathania,Sai Mukund Ramakrishnan,Ganesh Bagler
Database. 2015; 2015: bav075
[Pubmed] | [DOI]
10 ß-Caryophyllene, a natural sesquiterpene, modulates carbohydrate metabolism in streptozotocin-induced diabetic rats
Rafeek Hidhayath Basha,Chandrasekaran Sankaranarayanan
Acta Histochemica. 2014;
[Pubmed] | [DOI]
11 The Apoptotic Effect of Plant Based Nanosilver in Colon Cancer Cells is a p53 Dependent Process Involving ROS and JNK Cascade
Shakti Ranjan Satapathy,Purusottam Mohapatra,Dipon Das,Sumit Siddharth,Chanakya Nath Kundu
Pathology & Oncology Research. 2014;
[Pubmed] | [DOI]
12 Antidiabetic plants used among the ethnic communities of Unakoti district of Tripura, India
Ramananda Ghosh Tarafdar,Sushmita Nath,Anupam Das Talukdar,Manabendra Dutta Choudhury
Journal of Ethnopharmacology. 2014;
[Pubmed] | [DOI]
13 Liquid chromatography mass spectrometry simultaneous determination of vindoline and catharanthine in rat plasma and its application to a pharmacokinetic study
Chongliang Lin,Jinzhang Cai,Xuezhi Yang,Lufeng Hu,Guanyang Lin
Biomedical Chromatography. 2014; : n/a
[Pubmed] | [DOI]
14 Rediscovering Medicinal Plantsæ Potential with OMICS: Microsatellite Survey in Expressed Sequence Tags of Eleven Traditional Plants with Potent Antidiabetic Properties
Jagajjit Sahu,Priyabrata Sen,Manabendra Dutta Choudhury,Budheswar Dehury,Madhumita Barooah,Mahendra Kumar Modi,Anupam Das Talukdar
OMICS: A Journal of Integrative Biology. 2014; 18(5): 298
[Pubmed] | [DOI]
15 Medicinal plant usage by traditional medical practitioners of rural villages in Chuadanga district, Bangladesh
M. Mizanur Rahman,Gazi Ziaul Haque Masum,Priyanka Sharkar,Shamima Nasrin Sima
International Journal of Biodiversity Science, Ecosystem Services & Management. 2013; 9(4): 330
[Pubmed] | [DOI]
16 Ethnomedicinal Importance of the Plants in Villages in Kushtia Sador and Mirpur Upozila, Bangladesh
Priyanka Sharkar,M. Mizanur Rahman,Gazi Ziaul Haque Masum,Md. Abu Nayeem,Md. Mozaffour Hossen,Abul Kalam Azad
Journal of Herbs, Spices & Medicinal Plants. 2013; 19(4): 401
[Pubmed] | [DOI]
17 Natural product vindoline stimulates insulin secretion and efficiently ameliorates glucose homeostasis in diabetic murine models
Xin-gang Yao,Fanglei Chen,Ping Li,Lingling Quan,Jing Chen,Liang Yu,Hong Ding,Chenjing Li,Lili Chen,Zhaobing Gao,Ping Wan,Lihong Hu,Hualiang Jiang,Xu Shen
Journal of Ethnopharmacology. 2013; 150(1): 285
[Pubmed] | [DOI]
18 Rhinacanthus nasutus Ameliorates Cytosolic and Mitochondrial Enzyme Levels in Streptozotocin-Induced Diabetic Rats
Pasupuleti Visweswara Rao,K. Madhavi,M. Dhananjaya Naidu,Siew Hua Gan
Evidence-Based Complementary and Alternative Medicine. 2013; 2013: 1
[Pubmed] | [DOI]
19 Abnormalities in carbohydrate and lipid metabolisms in high-fructose dietfed insulin-resistant rats: amelioration by Catharanthus roseus treatments
Karuna Rasineni,Ramesh Bellamkonda,Sreenivasa Reddy Singareddy,Saralakumari Desireddy
Journal of Physiology and Biochemistry. 2013; 69(3): 459
[Pubmed] | [DOI]
20 Ethanolic extract of Commiphora mukul gum resin attenuates streptozotocin-induced alterations in carbohydrate and lipid metabolism in rats
Ramesh, B. and Karuna, R. and Sreenivasa Reddy, S. and Sudhakara, G. and Saralakumari, D.
EXCLI Journal. 2013; 12: 556-568
21 Catharanthine dilates small mesenteric arteries and decreases heart rate and cardiac contractility by inhibition of voltage-operated calcium channels on vascular smooth muscle cells and cardiomyocytes
Jadhav, A. and Liang, W. and Papageorgiou, P.C. and Shoker, A. and Kanthan, S.C. and Balsevich, J. and Levy, A.S. and Heximer, S. and Backx, P.H. and Gopalakrishnan, V.
Journal of Pharmacology and Experimental Therapeutics. 2013; 345(3): 383-392
22 Rhinacanthus nasutus ameliorates cytosolic and mitochondrial enzyme levels in Streptozotocin-induced diabetic rats
Visweswara Rao, P. and Madhavi, K. and Dhananjaya Naidu, M. and Gan, S.H.
Evidence-based Complementary and Alternative Medicine. 2013; 2013(486047)
23 Targeting of Rho Kinase Ameliorates Impairment of Diabetic Endothelial Function in Intrarenal Artery
Hongping Yin,Hailong Ru,Liping Yu,Yanhua Kang,Guohua Lin,Chuanfei Liu,Lixian Sun,Liyun Shi,Qinghua Sun,Cuiqing Liu
International Journal of Molecular Sciences. 2013; 14(10): 20282
[Pubmed] | [DOI]
24 Protective effect of ethanolic extract of Commiphora mukul gum resin against oxidative stress in the brain of streptozotocin induced diabetic wistar male rats
Sudhakara, G. and Ramesh, B. and Mallaiah, P. and Sreenivasulu, N. and Saralakumari, D.
EXCLI Journal. 2012; 11: 576-592
25 Medico-ethnobotanical inventory of Renukoot forest division of district Sonbhadra, Uttar Pradesh, India
Singh, A. and Singh, G.S. and Singh, P.K.
Indian Journal of Natural Products and Resources. 2012; 3(3): 448-457
26 Antidiabetic and hypolipidemic activity of Citrus medica Linn. seed extract in streptozotocin induced diabetic rats
Sah, A.N. and Joshi, A. and Juyal, V. and Kumar, T.
Pharmacognosy Journal. 2011; 3(23): 80-84
27 Medicinal plants used by folk medicinal practitioners of six villages in Thakurgaon District, Bangladesh
Sarker, S. and Seraj, S. and Sattar, M.M. and Haq, W.M. and Chowdhury, M.H. and Ahmad, I. and Jahan, R. and Jamal, F. and Rahmatullah, M.
American-Eurasian Journal of Sustainable Agriculture. 2011; 5(3): 332-343
28 Phytomedicine: An ancient approach turning into future potential source of therapeutics
Pandey, M., Debnath, M., Gupta, S., Chikara, S.K.
Journal of Pharmacognosy and Phytotherapy. 2011; 3(2): 27-37
29 Attenuation of nonenzymatic glycation, hyperglycemia, and hyperlipidemia in streptozotocin-induced diabetic rats by chloroform leaf extract of Azadirachta indica
Gutierrez, R.M.P. and Gomez, Y.G.Y. and Guzman, M.D.
Pharmacognosy magazine. 2011; 7(27): 254
30 Medicinal plants used by the folk medicinal practitioners of Bangladesh: a randomized survey in a village of Narayanganj district
Karim, M.S. and Rahman, M.M. and Shahid, S.B. and Malek, I. and Atiqur, M. and Rahman, S.J. and Jahan, F.I. and Rahmatullah, M.
American-Eurasian Journal of Sustainable Agriculture. 2011; 5(4): 405-414
31 Effects of a Mixture Of Salacia reticulata W. and Catharanthus roseus L. Extracts in Streptozotocin-Induced Juvenile Diabetic Rats
Rajashree, R. and Bhat, P.P. and Ravishankar, MV
J Physiol. 2011; 24(1): 5-8
32 Effect of the hydro-methanolic (2: 3) extract of the bark of Tectona grandis L. on the management of hyperglycemia and oxidative stress in streptozotocin-induced diabetes in rats
Bera, S. and Chatterjee, K. and Ali, KM and Ghosh, D. and others
Journal of Natural Pharmaceuticals. 2011; 2(4): 196
33 Antidiabetic and Hypolipidemic Activity of Citrus medica Linn. Seed Extract in Streptozotocin Induced Diabetic Rats
Archana N. Sah,Amit Joshi,Vijay Juyal,Tirath Kumar
Pharmacognosy Journal. 2011; 3(23): 80
[Pubmed] | [DOI]
34 Preventive effect of Catharanthus roseus (Linn.) against high-fructose diet-induced insulin resistance and oxida-tive stress in male Wistar rats
Karuna Rasineni,Saralakumari Desireddy
Journal of Diabetes Mellitus. 2011; 01(03): 63
[Pubmed] | [DOI]
35 A Review of Recent Investigations on Medicina l Herbs Possessing Anti-Diabetic Properties
Aggarwal, N.
J Nutrition Disorder Ther. ; 1(102): 2


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