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Year : 2009  |  Volume : 1  |  Issue : 4  |  Page : 234-237 Table of Contents     

Development and Evaluation of Polyherbal Formulations for Hair Growth Potential

1 Department of Pharmacognosy, NRI Institute of Pharmaceutical Sciences, 3, Sajjan Singh Nagar, Bhopal - 462 026, India
2 Department of Pharmacognosy, Nandha College of Pharmacy,Perundurai Main Road, Erode - 638 052, India

Date of Submission14-Apr-2009
Date of Decision02-Jun-2009
Date of Acceptance02-Jun-2009
Date of Web Publication2-Jan-2010

Correspondence Address:
Vaishali Rathi
Department of Pharmacognosy, NRI Institute of Pharmaceutical Sciences, 3, Sajjan Singh Nagar, Bhopal - 462 026
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Source of Support: None, Conflict of Interest: None

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The present study is an effort to formulate and evaluate hair growth promoting activity of three polyherbal formulations. Polyherbal formulations were prepared using extract of Cicer arietinum Linn., Ocimum sanctum Linn. and Cyperus rotundus Linn. in various ratios to obtained the best formulation The extract incorporated into cream were applied topically on shaved skin of rats and primary skin irritation test, hair growth initiation time, completion time, hair length and diameter were recorded. The ratio of Cicer arietinum Linn., Ocimum sanctum Linn. and Cyperus rotundus Linn. in 1:2:3 showed excellent hair growth activity comparable to standard.

Keywords: Alopecia, Polyherbal, Hair growth, Herbal formulation

How to cite this article:
Rathi V, Rathi JC, Tamizharasi S. Development and Evaluation of Polyherbal Formulations for Hair Growth Potential. Phcog Res 2009;1:234-7

How to cite this URL:
Rathi V, Rathi JC, Tamizharasi S. Development and Evaluation of Polyherbal Formulations for Hair Growth Potential. Phcog Res [serial online] 2009 [cited 2021 Jun 22];1:234-7. Available from: http://www.phcogres.com/text.asp?2009/1/4/234/58106

   Introduction Top

Alopecia is a universal problem, having affected both sexes of all races to different extents for as long as mankind has existed. It has been suggested that alopecia could have an adverse effect on physiological life and self esteem between both the genders [1] . Alopecia effects approximately 50% of men over 40 years of age and may also affect just as many as women. The majority of men and women (90%) or more want to reverse, halt hair loss. Alopecia is a synonym of baldness, involves absence or loss of hair, especially of the head. Androgens are well known to cause regression and balding on the scalp in genetically disposed individuals. Alopecia has also been observed as major side effect of anticancer drugs, immunosuppressant and many other drug treatments. Minoxidil, a drug of scientific origin was scientifically proved for the treatment of alopecia [2] . Though the side effect associated with this drug has limited its pharmacological benefits hence the drug of plant origin is necessary to replace the synthetic one. India is a repository of medicinal plants [3],[4] . Besides healthcare, herbs are also used for beautification of the body and for preparation of various cosmetics [5] . In traditional system of medicine, many plants and herbal formulations are reported for hair growth promotion [6],[7],[8],[9],[10] but lack of sound scientific backing and information limits their use.

The present study is an effort to formulate and evaluate hair growth promoting activity of polyherbal formulation, which include various concentrations of Cicer arietinum Linn., Ocimum sanctum Linn. and Cyperus rotundus Linn. The herbs Cicer arietinum Linn and Cyperus rotundus Linn. were selected on the basis of their traditional use [11],[12] and Ocimum sanctum Linn. was selected based on its anti-androgenic property [13] .

   Materials and Methods Top

Plant material

The leaves of Cicer arietinum Linn., whole plant of Ocimum sanctum Linn. and roots of Cyperus rotundus Linn. were collected in the month of October locally from Bhopal. The plants were authenticated at Department of Pharmacy, Barkatullah University, Bhopal. The plants were dried under shade.


Dried powered drug were taken and maceration was done by keeping them in 95% alcohol for 7 days with occasional stirring. After filtration, double maceration was done for next three days with 95% alcohol. The solvent was removed under reduced pressure and the extract obtained was air dried.


Herbal hair creams were prepared by fusion method using o/w type base [14] . The formula of base contains glyceryl mono stearate 9% w/w, liquid paraffin (light) 20% w/w, cetyl alcohol 15% w/w, beeswax 15% w/w, propyl and methyl paraben 0.15% w/w, glycerol 4.5% w/w and water 59% w/w. The 5% extract mixture of Cicer arietinum Linn., Ocimum sanctum Linn. and Cyperus rotundus Linn. in various ratio as shown in [Table 1] were incorporated in the base to obtain 5% herbal cream F 1 , F 2 , and F 3 respectively.


rats, weighing 120- 150 g were used for hair growth activity. The study was approved by Institutional Animal Ethical Committee, Barkatullaha University, Bhopal. Animals were placed in cages and kept in standard environmental conditions, fed with standard diet ad libitum and allowed free access to drinking water. The prepared formulations were assessed for hair growth studies.

Primary skin irritation test

The rats were divided into five groups of six rats each. A 4cm 2 area of dorsal portion of all the rats were shaved and wiped with surgical spirit. Measured quantity of formulation of F 1 , F 2 , and F 3 were applied over the site. The test sites were observed for erythema and edema for 48 h after application [15] .

Hair growth activity

The rats were divided into five groups of six rats each. A 4 cm 2 area of dorsal portion of all the rats were shaved and wiped with surgical spirit. Hair remover was also applied over the shaved area to assure the removal of trace of hairs from denuded area. Group1 was kept as control, where there was no drug treatment. Group2 was treated as standard, where 2% minoxidil lotion (Mintop) was applied over the shaved area, once a day. The animals of remaining groups were given application of 5% cream of formulation, F 1 , F 2 and F 3 respectively. The treatment was continued for 30 days during which time, hair growth initiation (minimum time to initiate hair growth on denuded skin region) and completion time (time taken to completely cover the denuded skin region with new hair) were recorded for each group of animals [16] . Hair was plucked randomly from the shaved area of selected rats, from each group on 10 th , 20 th and 30 th day of the treatment and length and diameter of 24 hairs was measured [17] . The average length and diameter were determined. The determination and evaluation of these parameters have been considered as vital for accomplishment of hair growth. It is considered that these parameters are accomplishing the concept of hair growth.

   Results and Discussion Top

Primary skin irritation test

This test was conducted to evaluate the irritancy of the prepared formulations on intact skin of rats. None of the prepared formulations showed any erthyema or edema, indicating that the prepared formulations were non- irritant on the skin of rats.

Hair growth activity

In control group animals, initiation of hair growth in denuded area was observed in second week. Hair growth initiation was noted in the first week in rats of minoxidil treated standard group. The formulation F 3 exhibited hair growth initiation on 7 th day and F 2 on 9 th day whereas with formulation F 1 , hair growth initiation time was reduced to 4 th day. Similarly the time taken for complete hair growth on shaved area was affected with minoxidil treatment as well as treatment with formulations. Complete hair growth with minoxidil and control group was observed in 20 and 24 days respectively. In formulation F 2 complete hair growth occurred after 22 days, in F 3 after 19 days, and in F 1 it was reduced to 18 days [Table 2]. In comparison to control for formulation F 1 the whole denuded area was covered with hair during the 4 th week [Figure 1]. The experiment thus clearly demonstrates hair growth promoting activity in the developed formulations. The length of the hair began to increase until the end of the treatment course [Table 3]. The formulation F 1 produced a greater effect on the length of hair when compare to other group being 9.08 mm at the end of the course, compare to 7.88 mm in the F 2 , 8.38 mm in F 3 and 8.5 mm in standard. This may be due to the premature switching of follicles from the telogen to anagen phase of hair growth cycle [18] . On 30 th day, in control animal diameter of hair was found 0.0279 mm, in standard it was 0.0458 mm, in formulation F 2 and F 3 it was 0.0304 mm and 0.0408 mm respectively, but in formulation F 1 , it was highest around 0.0479 mm [Table 4].

   Conclusion Top

Among the various formulations, the formulation F 1 , showed better growth initiation and hair growth completion time at the same time formulation F 1 , showed remarkable improvement in hair length and diameter compare to control, standard and other formulations. Hence it can be concluded that the extract combination F 1 proved excellent growth activity and the formulation F 1 , might be hold a promise of potential herbal alternative for synthetic drugs used for alopecia.

   References Top

1.Bhalerao S.S. and Solanki N.H. Therapeutic approaches to management of common baldness. Indian Drug. 39(11): 567-573 (2002).  Back to cited text no. 1      
2.Reddy M.S., Mautalik S. and Rao V.G. Preparation and evaluation of mi­noxidil gels for topical application in alopecia. Indian J Pham Sci. 68(4): 432-436 (2006).  Back to cited text no. 2      
3.Saraf S., Pathak A.K. and Dixit V.K. Hair growth promoting activity of Tridax procumbens. Fitoterapia. 62: 495-498 (1991).  Back to cited text no. 3      
4.Ansari S.H. and Ali M. Hair care and herbal drug. Indian J Nat Prod. 13(1): 3-5 (1997).  Back to cited text no. 4      
5.Aburjai T. and Natsheh F.M. Plants used in cosmetics. Phytother Res. 17: 987-1000 (2003).  Back to cited text no. 5      
6.Matsuda H., Yamazaki M., Asanuma Y. and Kubo M. Promotion of hair growth by Ginseng radix on cultured mouse vibrissal hair follicles. Phytother Res. 17: 797-800 (2003).  Back to cited text no. 6      
7.Grindlay D. and Reynolds T. The aloe vera phenomenom: a review of the properties and modern uses of the leaf parenchyma gel. J Ethnopharm. 16: 117-151 (1986).  Back to cited text no. 7      
8.Dweck A.C. African plants. Cosmet Toiletries. 112: 41-51 (1997).  Back to cited text no. 8      
9.Kamimura A. and Takahashi T. Procyanidin B-2, extracted from apples, promote hair growth: a laboratory study. British Journal of Dermatology. 146: 41-51 (2002).  Back to cited text no. 9      
10.Rathi V., Rathi J.C., Tamizharasi S. and Pathak A.K. Plants used for hair growth promotion: A review. Phcog Rev. 2(3): 165-167 (2008).  Back to cited text no. 10      
11.Kirtikar K.P. and Basu B.D., Indian Medicinal Plants, Vol I, (International Book Distributors, Dehradun, 1995) 768-769.  Back to cited text no. 11      
12.Ayurvedic Pharmacopoeia of India, Vol III, (Govt of India, Ministry of Health and Welfare, New Delhi, 1999) 129.  Back to cited text no. 12      
13.Das S.K. and Vasudevan D.M. Tulsi: The Indian holy power plant. Nat Prod Rad. 5(4): 279-280 (2006).  Back to cited text no. 13      
14.Dixit V.K., Adhirajan N. and Gowri C. Development and evaluation of herbal formulations for hair growth. Indian Drugs. 38(11): 559-563 (2001).  Back to cited text no. 14      
15.Patni P., Varghese D., Balekar N. and Jain D.K. Formulation and evalua­tion of herbal hair oil for alopecia management. Planta Indica. 2(3): 27-30 (2006).  Back to cited text no. 15      
16.Roy R.K., Thakur M. and Dixit V.K. Effect of Cuscuta reflexa Roxb. On hair growth activity of albino rats. Indian Drugs. 43: 951-956 (2006).  Back to cited text no. 16      
17.Adirajan N., Ravikumar T., Shanmugasundaram N. and Babu M. In vivo and in vitro evaluation of hair growth potential of Hibiscus rosa sinensis Linn. J Ethanpharm. 88: 235-239 (2003).  Back to cited text no. 17      
18.Philpot M.P, Green M.R and Kealey T. Rat hair follicle growth in vitro. Br J Dermatol. 127: 600-607 (1992).  Back to cited text no. 18      


  [Figure 1]

  [Table 1], [Table 2], [Table 3], [Table 4]


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