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RESEARCH ARTICLE
Year : 2009  |  Volume : 1  |  Issue : 3  |  Page : 152-156 Table of Contents     

Chemical Investigation of Aerial Parts of Gmelina asiatica Linn by GC-MS


1 Department of Pharmacy, Annamalai University, Annamalai Nagar-608002, Tamil Nadu, India
2 Food testing laboratory, Indian institute of crop processing technology, Thanjavur-613005, Tamil nadu, India

Date of Submission16-Feb-2009
Date of Acceptance13-Apr-2009
Date of Web Publication2-Jan-2010

Correspondence Address:
N J Merlin
Department of Pharmacy, Annamalai University, Annamalai Nagar-608002, Tamil Nadu
India
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Source of Support: None, Conflict of Interest: None


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   Abstract 

This study was carried out to analyze the active constituents present in aerial parts of Gmelina asiatica Linn. (Verbenaceae). Twenty-two compounds in chloroform extract and 12 compounds in ethanolic extract were identified by Gas Chromatography - Mass Spectrometry (GC-MS) analysis. 1, 2-Benzenedicarboxylic acid, diisooctyl ester (31.22 %) was the prevailing compound in chloroform extract and Monolinoleoylglycerol trimethylsilyl ether (38.51%) was the major constituent of ethanolic extract. This is the first report of identification of active constituents from aerial parts of Gmelina asiatica by GC-MS.

Keywords: Gmelina asiatica (Verbenaceae), GC-MS, 1,2-Benzenedicarboxylic acid diisooctyl ester, Monolinoleoylglycerol trimethylsilyl ether


How to cite this article:
Merlin N J, Parthasarathy V, Manavalan R, Kumaravel S. Chemical Investigation of Aerial Parts of Gmelina asiatica Linn by GC-MS. Phcog Res 2009;1:152-6

How to cite this URL:
Merlin N J, Parthasarathy V, Manavalan R, Kumaravel S. Chemical Investigation of Aerial Parts of Gmelina asiatica Linn by GC-MS. Phcog Res [serial online] 2009 [cited 2020 Dec 2];1:152-6. Available from: http://www.phcogres.com/text.asp?2009/1/3/152/58128


   Introduction Top


Most traditional medicines are developed from nature. They have not yet fulfilled the scientific requirements so as to be classified as modern medicines [1],[2] . For purposes of scientific back up, a study is needed to examine their bioactive components, their efficacy and safety [3],[4] . Usually, most components that are useful for medicinal purposes are secondary metabolites [5][6] .

In the development of medicinal plant industry, plant medicines are classified into three groups: herbs (Jammu), standardized extracts and phyto­pharmaceuticals [7] . There are strict requirements for standardizing the extracts. Some of them include correctness and proven restorative power, uniformity of active constituents, their efficacy, safety and assurance, both in quality and quantity [8],[9] .

Gmelina asiatica L (Verbenaceae) popularly known as Nilakkumil in Tamil and Gopabhandra in Sanskrit, is a large straggling shrub found in South India. Its roots are used for the treatment of gonorrhoea, catarrh of the bladder, rheumatism and for purification of the blood [10] . Since this plant is claimed to be useful in the treatment of rheumatism, it is said to possess anti­inflammatory action [11] . The root of the plant also has potent hypoglycaemic activity [12] . The aerial parts of Gmelina asiatica have antimicrobial activity [13] . Extensive phytochemical studies have been carried out in roots but thorough phytochemical work has not been carried out in the aerial parts of this plant. Since there are no reports on the phytochernical aspects of Gmelina asiatica aerial parts, it was chosen as the subject for this study. The aim of this paper is to validate a rapid method for the quantitative determination of organic compounds in the aerial parts of Gmelina asiatica using rapid fingerprint procedure.


   Materials and Methods Top


Plant material

Aerial parts of the Gmelina asiatica were collected from Therkkumalai Estate, Courtallam Hills, Western Ghats, Tamil Nadu, India and identified by Dr.V.Chelladurai, Research Officer, Central Council for Research in Ayurveda and Siddha, Palayamkottai. Voucher specimens were prepared and preserved in the Department of Pharmacognosy, KMCP, Madurai for further reference (Voucher specimen no. KMCP/GA/23).

Preparation of extract

Aerial parts of Gmelina asiatica were shade dried and defatted with petroleum ether. The defatted material was extracted using soxhlet apparatus with chloroform and 95% ethanol. The extracts were then filtered through Whatmann titer paper No.41 along with 2gm sodium sulfate to remove the sediments and traces of water in the filtrate. Before filtering, the filter paper along with sodium sulphate was wetted with chloroform and 95% ethanol respectively. The filtrate was then concentrated by bubbling nitio n gas into the solution. The extract contained both polar and non-polar phyhocomponents of the plant material used. 2 ml sample of these solutions was employed for GUMS analysis.

GC Analysis

GC-MS analysis was carried out on a GC darns 500 Perkin Elmer system comprising a AOC-20i autosamper and gas chromatograph interfaced to a mass spectrometer (GC-MS) instrument employing the following conditions: column bite-1 fused silica capillary column (3Qnm)0.25mm ID x1ylh df, composed of 100% Dime" pity silexane), operating in electron impact mode at 70 eV; helium (99.999 was used as carrier gas at a constant flow of lml/min and an injection volume of 0.5 N was employed (split ratio of10:1) injector temperature 250° C ion-source temperature 280 °C. The oven temperature was programmed from 110 °C (isothermal for 2 min), with an increase of 10°C/min, to 200°C, then 5°C/min to 280°C, ending with a 9 min isothermal at 280°C. Mass spectra were taken at 70 eV,* a scan interval of 0.5 secords and fragments from 40 to 550 Da.

Identification of Components

Interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST) haing more than 62,000 patterns. The spectrum of the unknown component was compared with the spectrum of the known components stored in the NIST library. The name, molecular weigit and structure of the components of the test materials was ascertaired.


   Results and Discussion Top


The compunds present in the chloroform aid ethanolic extracts of Gmelina asiatica were identified by GC-MS analysis [Figure 1] and [Figure 2].The active principles with their retention time(RT), molecular formula, molecular weignt (MW) and concentration (%) in the chloroform and ethanolic extracts of Gmelina asiatica are presented in [Table 1] and [Table 2]. Twenty-two compounds were identified in cNoroform extract. The prevailing compound in chloroform extract was 1,2­Benzeredicarboxylic acid, diisooctyf ester (31.22 %). [Figure 3] and [Figure 4] show mass spectrum and structures of this compound which is suggested to be a plasticizer compound and is used as an antimicrobial and antifouling agent.

Nine compounds were identified in ethandic extract of the plant. Monolinoleoylglycerol trimethylsilyl ether, a steroid compound was identified by GC-MS andyss as the main constituent (38.51%) of ethenclic extract. [Figure 5] and [Figure 6] show the mass spectrum and structure of this compound, which is suggested to be a steroid compound and is used as an anti-microbia, anti-inflammatory, anti-arthritic, anti-diuretic and an anti-­asthmatic agent. Based on the chemical nature and percentages of compounds present, in the chloroform and ethanolic extracts, they may contribute to varying.


   Conclusion Top


This investigation has helped to identify the compounds present in the aerial parts of Gmelina asiatica, a hitherto uninvestigated species. Evaluation of pharmacological activity in the chloroform and ethanolic extracts is in progress.

 
   References Top

1.S. Gupta. Prospects and perspectives of natural plants products in medicine. Indian J Pharnuacol. 26:1-12 (1994).  Back to cited text no. 1      
2.E. Quisumbing. Medicinal Plants of the Philippines. (Manila Bureau of Printing 1954) pp: 1234.  Back to cited text no. 2      
3.J. R Dipalma. Drills Pharmacology Medicine. 4 5 Edn. (Mc Grave -Hill Book Ltd. London 1971) pp: 21-43.  Back to cited text no. 3      
4.N.R. Farnsworth, A.S. Akarela, D.D. Bingel, Z.G. Soejarto. Medicinal plants in therapy. Bull. WHO. 965-981(1985).  Back to cited text no. 4      
5.L.S Padua, N. Bunyaprapkatsara, R.H.M.J Lemmens Medicinal and Poisonous Plants (Blachuys Publisher, Leiden 1999) pp:711.  Back to cited text no. 5      
6.Jamaran. The role of science and technology on the development of medicinal plant agroindustry. Proceeding of Coordination and Strategic Consultation on the development of Medicinal Plant Agroindustty, Bogor Agriculture University, Bogor pp: 233(1995).  Back to cited text no. 6      
7.Anonymous. Phytofan iaca and Directive of Phytofarmaca. Ditwasot. Department of Health, RI. Jakarta, pp:63(1982).   Back to cited text no. 7      
8.L.G. Corral, L.S. Post, T.J. Montville, Antimicrobial activity of sodium bicartonate. J. Food Sci. 53:11-16 (1988).  Back to cited text no. 8      
9.R.D. Guenero, L.A. Guerrero, L.L. Garcia. Use of indigenous plants as sources of fish toxicants for pond management in the Philippines. Philippines Technol. J. 15:15-18 (1990).  Back to cited text no. 9      
10.K.R. Kirtikar, B.D. Basu, Indian Medicinal Plants, vol. II 2nd edition., (International Book Distributors, Dehradun, 1984) pp.1273.  Back to cited text no. 10      
11.T. Syed Ismail, S. Gopalakrishnan, V. Hazeena Begum. Indian J. Pharmacol. 29:306-309 (1997).  Back to cited text no. 11      
12.R. Kasivisvanath, A. Rarnesh, K Easwar Kumar. Hypoglycemic and Antihyperglycemic Effect of Gmelina asiatica Linn. in Normal and in Alloxan Induced Diabetic Rats. Biol Pharm. Bull. 28(4):729-732(2005).  Back to cited text no. 12      
13.M. Sudhacuu Ch.V. Rao, P.M. Rao, D.B. Raju. Evaluation of antimicrobial activity of Cleome viscose and Gmelina asiatica. Fitoterapia. 77(1):47-49 (1996).  Back to cited text no. 13      


    Figures

  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]
 
 
    Tables

  [Table 1], [Table 2]



 

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