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The Effect of Extracts of Andrographis paniculata Aerial Parts on Rat Thoracic Aorta
S Raghava Naidu1, Amirin Sadikun2, Mohd. Zaini Asmawi3
1 Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang, 11800; Island College of Technology, Department of Pharmacy, Sungai rusa, Balik pulau, 11000, Pulau Pinang, Malaysia
2 Department of Pharmaceutical chemistry, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang, 11800, Malaysia
3 Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang, 11800, Malaysia
Correspondence Address:
S Raghava Naidu
Department of Pharmacology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, Pulau Pinang, 11800; Island College of Technology, Department of Pharmacy, Sungai rusa, Balik pulau, 11000, Pulau Pinang
Malaysia
 Source of Support: None, Conflict of Interest: None  | Check |
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Andrographis paniculata (AP), (Burf.) Nees. of Acanthaceae, has been used for centuries in Asia to treat GI tract and upper respiratory tract infections, fever, herpes, sore throat, and a variety of other chronic and infectious diseases. AP has cardio protective property and is familiarly known as "King of Bitters". The present study was aimed to investigate the vasorelaxant effect of different solvent extracts of AP on rat thoracic aorta. Petroleum ether, chloroform, and methanol extracts of AP are used in this study. Experiments are performed on male Sprague-Dawley (SD) rats for possible vasorelaxing activity of AP. Cumulative dose response curves are recorded by using isometric force displacement transducer Model FT-03 and it is connected to Grass Polygraph Model 79D. Additionally, HPLC study of chloroform extract of AP is conducted and compared with commercially available standard andrographolide. Petroleum ether and chloroform extract of AP is first suspended in 1% (v/v) DMSO then volume made up with Krebs solution. The average of responses to each concentration of the agonist is plotted on the ordinate the logarithm of the concentration of the agonist on the abscissa. Among all these extracts of AP, chloroform extract 80 and 160 μg/mL is found to be the highly significant (P<0.001) vasorelaxant effect on norepinephrine induced contraction on rat thoracic aortic ring preparations. |
