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  Indian J Med Microbiol
 

Figure 6: The maximal nitric oxide concentration (a), the maximal ONOO− concentration (b) and the ratio of maximal nitric oxide concentration to maximal ONOO− concentration (c) measured from HUVECs after stimulation with calcium ionophore (1.0 μM) in Hank's balanced salt solution buffer. The cells were incubated with 0.03% of different CARDIO components for 6 h in serum-free media: Control (without treatment (gray bars), (1) All components (dashed bars), (2) 3000 mg L-arginine, 1000 mg L-citrulline and 1500 IU vitamin D3(open bars), (3) antioxidants (solid bars). *P < 0.05; **P < 0.005; versus control group (unpaired, two-tailed Student's t-test). Data shown are the means ± standard deviation, n = 5

Figure 6: The maximal nitric oxide concentration (a), the maximal ONOO− concentration (b) and the ratio of maximal nitric oxide concentration to maximal ONOO− concentration (c) measured from HUVECs after stimulation with calcium ionophore (1.0 μM) in Hank's balanced salt solution buffer. The cells were incubated with 0.03% of different CARDIO components for 6 h in serum-free media: Control (without treatment (gray bars), (1) All components (dashed bars), (2) 3000 mg L-arginine, 1000 mg L-citrulline and 1500 IU vitamin D<sub>3</sub>(open bars), (3) antioxidants (solid bars). *<i>P</i> < 0.05; **<i>P</i> < 0.005; versus control group (unpaired, two-tailed Student's <i>t</i>-test). Data shown are the means ± standard deviation, <i>n</i> = 5