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  Indian J Med Microbiol
 

Figure 3: Maximal concentration of nitric oxide produced by HUVECs after stimulation with calcium ionophore (1.0 μM) in Hank's balanced salt solution buffer. The cells were incubated with CARDIO 0.03% (100 nmol/L of D3) for 3, 6, 12, and 24 h in serum-free media. *P < 0.05; **P < 0.005 versus control group (unpaired, two-tailed Student's t-test). Data shown are the means ± standard deviation, n = 5

Figure 3: Maximal concentration of nitric oxide produced by HUVECs after stimulation with calcium ionophore (1.0 μM) in Hank's balanced salt solution buffer. The cells were incubated with CARDIO 0.03% (100 nmol/L of D<sub>3</sub>) for 3, 6, 12, and 24 h in serum-free media. *<i>P</i> < 0.05; **<i>P</i> < 0.005 versus control group (unpaired, two-tailed Student's <i>t</i>-test). Data shown are the means ± standard deviation, <i>n</i> = 5