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  Indian J Med Microbiol
 

Figure 7: Treatment with Uncaria tomentosa and ionizing radiation promotes cellular apoptosis. THP-1 cells were treated with Uncaria tomentosa and/or lipopolysaccharide for 24 h and/or ionizing radiation and then incubated for a further 24 h. (a) Cell lysates were obtained and duplicate blots probed for caspase-3 or PARP with antibodies which detect both the cleaved and uncleaved forms. (b) DNA fragments were isolated from the cells, precipitated, and then subjected to electrophoresis on agarose gels stained with ethidium bromide. M shows the DNA marker. (c) Treated cells stained with Hoechst 33342 and fixed on glass coverslips were visualized on a fluorescence microscope

Figure 7: Treatment with <i>Uncaria tomentosa</i> and ionizing radiation promotes cellular apoptosis. THP-1 cells were treated with <i>Uncaria tomentosa</i> and/or lipopolysaccharide for 24 h and/or ionizing radiation and then incubated for a further 24 h. (a) Cell lysates were obtained and duplicate blots probed for caspase-3 or PARP with antibodies which detect both the cleaved and uncleaved forms. (b) DNA fragments were isolated from the cells, precipitated, and then subjected to electrophoresis on agarose gels stained with ethidium bromide. M shows the DNA marker. (c) Treated cells stained with Hoechst 33342 and fixed on glass coverslips were visualized on a fluorescence microscope