Pharmacognosy Research

ORIGINAL ARTICLE
Year
: 2015  |  Volume : 7  |  Issue : 4  |  Page : 309--313

Alpha-amylase inhibitory activity and phytochemical study of Zhumeria majdae Rech. f. and Wendelbo


Behnaz Mirshafie1, Najmeh Mokhber-Dezfouli1, Azadeh Manayi1, Soodabeh Saeidnia1, Yousef Ajani2, Ahmad Reza Gohari1 
1 Medicinal Plants Research Center, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Pharmacognosy, Institute of Medicinal Plant, ACECER, Tehran, Iran

Correspondence Address:
Soodabeh Saeidnia
Medicinal Plants Research Center, Tehran University of Medical Sciences, P.O. Box 14155 6451, Tehran
Iran

Background: Zhumeria majdae (Lamiaceae) is an endemic species growing in the South parts of Iran especially Hormozgan province. The plant is so-called Mohrekhosh locally and widely used for medicinal purposes including stomachache and dysmenorrhea. Objective: In order to separation and identification of the main flavonoid glycosides of the plant (aerial parts including leaves, stems, flowers, and fruits were used) and evaluation of its alpha-amylase inhibitory (AAI) activity, methanolic extract was prepared and fractionated to botanolic portion. Materials and Methods: Isolation of the main compounds of the butanol extract of the plant have been performed using different column chromatography methods such as high-performance liquid chromatography (C 18 column) and Sephadex LH-20 as well. The isolated compounds were identified by Hydrogen-1 nuclear magnetic resonance and Carbon-13 nuclear magnetic resonance spectra and comparison with those reported in previous literature. Moreover, inhibitory activity of the butanolic extract of the plant against alpha-amylase enzyme was examined in different concentrations (15-30 mg/mL), where acarbose used as a positive control. Results: Three flavonoid glycosides: Linarin (1), hispidulin-7-O-(4-O-acetyl-rutinoside) (2), hispidulin-7-O-rutinoside (3) were successfully identified in the extract. The activity of alpha amylase enzyme was dose-dependently suppressed by the butanol extract. The extract exhibited the highest inhibition at 30 mg/mL toward enzyme (77.9 ± 2.1%), while acarbose inhibited the enzyme at 20 mg/mL by 73.9 ± 1.9%. The inhibitory concentrations of 50% for the extract and acarbose were calculated at 24.5 ± 2.1 and 6.6 ± 3.1 mg/mL, respectively. Conclusion: Z. majdae contains glycosylated flavones and could be a good candidate for anti-diabetic evaluations in animal and clinical trials due to possessing AAI activity.


How to cite this article:
Mirshafie B, Mokhber-Dezfouli N, Manayi A, Saeidnia S, Ajani Y, Gohari AR. Alpha-amylase inhibitory activity and phytochemical study of Zhumeria majdae Rech. f. and Wendelbo .Phcog Res 2015;7:309-313


How to cite this URL:
Mirshafie B, Mokhber-Dezfouli N, Manayi A, Saeidnia S, Ajani Y, Gohari AR. Alpha-amylase inhibitory activity and phytochemical study of Zhumeria majdae Rech. f. and Wendelbo . Phcog Res [serial online] 2015 [cited 2019 Dec 13 ];7:309-313
Available from: http://www.phcogres.com/article.asp?issn=0974-8490;year=2015;volume=7;issue=4;spage=309;epage=313;aulast=Mirshafie;type=0