Year : 2009 | Volume
: 1 | Issue : 3 | Page : 157--161
In-vivo Antitussive Activity of Cressa cretica Linn. using Cough Model in Rodents
P Sunita1, S Jha2, SP Pattanayak3,
1 Government Pharmacy Institute, Govt. of Jharkhand, Bariatu, Ranchi – 834 009, India
2 Division of Pharmacognosy, Birla Institute of Technology (BIT), Mesra, Ranchi - 835 215, India
3 Division of Pharmacology, Birla Institute of Technology, Mesra, Ranchi - 835 215, India
Government Pharmacy Institute, Govt. of Jharkhand, Bariatu, Ranchi – 834 009
Cressa cretica Linn. Voigt. (Convolulaceae), has also been extensively used to get relief from asthma and cough by the indigenous people of India. In the present study the antitussive effect of the plant was evaluated in two different experimental models. The antitussive effect of aerosols of two different concentrations (2.5%w/v, 5%w/v)of methanolic extract of Cressa cretica Linn. (CME), codeine(0.03g/ml), and normal saline were tested by counting the numbers of coughs produced due to aerosols of citric acid 10 min after exposing the male guinea pigs to aerosols of different solutions (n=6). In another set of experiment CME was investigated for its therapeutic efficacy on a cough model induced by sulfur dioxide gas in mice. The results showed significant reduction of cough number obtained in the presence of both concentrations of CME and codeine. The antitussive effect on guinea pigs of higher concentration of CME was significantly (p<0.01) greater than those of lower concentration and the prototype antitussive agent codeine phosphate (p<0.01). It exhibited significant anti tussive activity as that of codeine phosphate, when compared with control in a dose dependent manner in sulfur dioxide gas induced cough model. The extract at 100, 200 and 400 mg/kg, p.o. showed inhibition of cough by 22.1, 34.35 and 55.44 % within 90 min of performing the experiment.
|How to cite this article:|
Sunita P, Jha S, Pattanayak S P. In-vivo Antitussive Activity of Cressa cretica Linn. using Cough Model in Rodents.Phcog Res 2009;1:157-161
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Sunita P, Jha S, Pattanayak S P. In-vivo Antitussive Activity of Cressa cretica Linn. using Cough Model in Rodents. Phcog Res [serial online] 2009 [cited 2020 Apr 9 ];1:157-161
Available from: http://www.phcogres.com/text.asp?2009/1/3/157/58129
Cressa cretica L. (Convolulaceae), popularly known as Rudanti' in Hindi is a widely grown halophytic plant. Different parts of the plant have been claimed to be valuable in a wide spectrum of diseases ,, . In earlier studies Cressa cretica Linn flowers exhibited cytotoxic and anti-inflammatory activity  . Cressa cretica is reported to be antibilous, antituberculosis, and expectorant  Shahat et al. yielded five flavonoids (quercetin, quercetin-3-0-glucoside, kaempferol- 3-0-rhamnoglucoside, and rutin) from the aerial parts of Cressa cretica  . It is also reported the fruits of Cressa cretica is a potential source of edible oil. The oil of C. cretica was free from any undesirable components and could safely be recommenced for human consumption. Also the oil is of similar in composition with respect to individual fatty acids of commercial oils.  . To substantiate its activity against cough and cold attack the present study was designed to investigate the effect of Cressa cretica Linn. extract on irritant aerosol induced coughing in male guinea pigs  and sulpher dioxide induced cough reflex in mice  .
Materials and Methods
Plant material and extraction
Cressa cretica was collected from Nalban island of Chilika lake, Orissa and was identified by Dr. H. 0. Brahmam, Senior Scientist, Natural product division, Institute of Mineral and Material Technology. A voucher specimen has been kept in our laboratory for future reference. The whole plant was shade-dried, powdered, passed through a 40-mesh sieve and finally subjected to extraction with methanol in a soxhlet apparatus. The solvent was removed under vacuum and a solid mass (16.73% w/w with respect to dry starting material) so obtained was stored in a desiccator and used for further experimental studies. The methanol extract of the whole plant was subjected for preliminary phytochemical screening to show the presence of steroid, alkaloid, glycoside, tannin, triterpenoid, carbohydrates and reducing sugar.
The experiments were carried out in male guinea pigs (400-450 g) and Swiss albino mice of either sex weighing 400-450g and 30-40g respectively. Animals were kept in the departmental animal house at 26 ± 2°C at relative humidity 44-55% and light dark cycles of 10 and 14 h, respectively. Animals were provided with rodent diet and water ad-libitum. The animal experiment was performed according to the institute's ethical committee approval and guidelines Reg no. 621/02/ac/CPCSEA of Birla Institute of Technology, Mesra, India.
Protocol for irritant aerosol induced ant itussive evaluation
Male guinea pigs, five in each group were used in the study (body weight 500-600 g). The method has been described by Forsberg et al.  . Unanaesthetized unrestrained animals were placed individually in a transparent test chamber, dimensions 30 cm x 20 cm x 20 cm and exposed to a nebulized aqueous solution of i.e. 0.1 g/ml of citric acid for 7min . The output of nebulizer was 0.65 ± 0.04 ml solution per minute and continued for 7 min. The same nebulizer was used throughout the experiment. During the last 5 min of the exposure, the animals were watched continuously by a trained observer, and the numbers of coughs were determined. Coughs could easily be distinguished from sneeze since there is a clear difference in sound as well as in behaviour of the animals  .
The above protocol was performed 10 min after exposing animals to aerosols of the following solutions for a period of 7 min (n=5):
Normal saline (baseline measurements).Codeine solution (0.03 g/ml, positive control).CME (2.5%, w/v).CME (5%, w/v).All the experiments were performed randomly with 2 h resting period between each two experiments
SO 2 induce antitussive evaluation
Antitussive effect against S0 2 -induced cough was evaluated by the method as described by Miyagoshi et al  . The experimental model is shown in [Figure 1], where V 1 is 500 mL three-necked flask containing aqueous saturated solution of sodium hydrogen sulphite.
By opening the stopcock of a burette V 2 , the concentrated sulphuric acid was introduced to generate S02 gas. The chemical reaction that occurred in the flask A is
2NaHSO 3 + H 2 SO 4 = 2SO 2 ↑+ Na 2 SO 4 + 2H 2 O
Previously, SO 2 gas was filled in V 1 and V 3 gas reservoirs, and then by opening the cocks 3 and 2, pressure in the gas reservoir V 3 was elevated which was recorded by the water manometer V 4 . Then the stopcock 2 was closed and stopcock 4 was opened slightly till the pressure in V 4 (11 mm i.d.) reached 75mm water, when the stopcock was closed. The procedure was operated in a draught.
The mice were divided into five groups, each containing 10 mice. One group served as a control group receiving only 2% v/v aqueous Tween 80 solution (10 ml/kg -1 , p.o.). Three groups were used for methanol extract of C. cretica (100, 200 and 400 mg kg -1 p.o.) and the remaining group was used for standard drug codeine phosphate (10, 20 and, 40mg kg-1 p.o.). Both the extract and codeine phosphate were suspended separately in 2% v/v aqueous Tween 80 solution. Initially, the cough responses of all groups of animals were observed (0 min) by placing the animals individually in a desiccator V 5 . The cocks 3, 6 and 5 were opened in order and when the pressure in V 4 became 0 mm of water, all the cocks were closed immediately. A certain amount of SO 2 gas (5ml which was kept constant throughout the experiment) was introduced in the desiccator in this way. After 1 min of introduction of the gas, the mice were taken out of the desiccator and the frequency of cough was observed for 5 min in an open-ended filter funnel with a stethoscope at the tip in which the mice were confined in this way the frequency of cough was observed for all animal groups at 0 min (before the drug administration) and at 30 - 60 - 90 - 120 - min interval (after the drug administration).
The frequency of cough produced by irritant-aerosol in guinea pigs were analyzed by one way ANOVA followed by Bonferroni's multiple comparison tests to compare all pairs of columns. The results are expressed as means ± standard mean errors (S.E.M.). The data obtained from sulfur dioxide-induced experiment were analyzed by one-way ANOVA followed by Dunnett's test for comparing between the control group and the various groups. Statistical significance was assumed at the 0.05 levels.
Aerosol induced antitussive evaluation
In the present study the antitussive effects of extracts from C. Cretica were evaluated using a standard method used previously by several investigators , . The results are depicted in [Figure 2]. Both concentrations of CME (2.5%w/v, 5%w/v) and the prototype drug, codeine phosphate (0.03 g/mL) caused significant reduction in cough number compared to base line value (ppcretica was significantly greater than those of Lower concentration (pSulpher dioxide induced antitussive evaluation
The effect of C. cretica extract on sulpher dioxide induced cough in experimental animals is shown in [Table 1]. It was observed that on exposure of the experimental animals (mice) to sulpher dioxide gas, the frequency of cough of control group remains more or less constant, i.e. it varies between 58.4 ± 1.21 and 62.67 ± 0.89 (mean ± SEM). But both in case of codeine phosphate and extract of C. cretica on oral administration, frequency of cough decreased in a dose- related manner. It was found that both the standard drug and extract (at different doses) produce maximum inhibition of cough reflex at 90 min after drug administration. A dose- dependent inhibition of cough was observed with CME and results were also comparable with the effect produced by codeine phosphate, a prototype antitussive agent. The results obtained with 100 - 200 - and 400 mg/kg doses of extract were statistically significant (p  or chemical irritation  and by electrical stimulation  of tracheal mucosa or by nerve stimulation  . Of all these methods, chemical or mechanical stimulation are more similar to the physiological event and also the experimental models generally used in man.
In the present study, the antitussive activity of CME has been compared with that of codeine against coughing induced in two different animal species by chemicals stimulation (irritant citric acid aerosol and SO 2 gas) stimulation. The extracts showed marked antitussive effect. The extract showed significant inhibition of cough like the standard drug (codeine phosphate) in dose-dependent manner; thus the extract might be acting via the central nervous system, but the exact mechanism of action can not be withdrawn from the preliminary study.
So from this study it can be concluded that on preliminary screening the extract of C. cretica produced a significant antitussive effect and thus established the claim of using the plant as an anticough agent in ancient folklore medicine. Further work relating to isolation and characterization of the active constituents present in the the plant and studies on various pharmacological evaluations, as well as evaluation of the mechanism of action for antitussive effect, is under way by our research team in our laboratory.
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