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ORIGINAL ARTICLE
Year : 2020  |  Volume : 12  |  Issue : 3  |  Page : 260-266

Screening of cytotoxic activity of hematite (α-Fe2O3) nanoparticles from Butea monosperma on MCF-7 cells


1 Department of Pharmacy Science, Creighton University, Medical Centre, Omaha, NE, USA
2 Department of Pharmaceutical Sciences, Utkal University, Bhubaneswar, Odisha, India

Correspondence Address:
Dr. Debasish Pradhan
Department of Pharmaceutical Sciences, Utkal University, Bhubaneswar, Odisha
USA
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pr.pr_115_19

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Background: Hematite (α-Fe2O3) has been used as an antimicrobial and disinfectant agent. Nevertheless, there are limited data about antitumor potential. This study was focused on investigating cytotoxic effects of Hematite (α-Fe2O3) from Butea monosperma flower extract on MCF -7 breast cancer cells and its mechanism of action. Materials and Methods: Thus, a green method was created for the synthesis of Hematite (α-Fe2O3) using an aqueous extract of B. monosperma flower. Synthesis of Hematite (α-Fe2O3) was described by different analytical techniques including ultraviolet-visible spectrophotometer, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transforms infrared spectroscopy. Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Reactive oxygen species (ROS) formation was measured using probe 2',7'-dichlorofluorescein diacetate and intracellular calcium (Cai2+) was evaluated with probe flu3-AM. Cells were treated with different concentrations of Hematite (α-Fe2O3) (1, 3, 6, 10, 15, 25, 50, and 100 μg/mL). Results: The results showed that Hematite (α-Fe2O3) hindered cell growth in a dose-dependent manner. Hematite (α-Fe2O3) appeared to have dose-dependent cytotoxicity against MCF-7 cells through activation of the ROS generation and an increase in the intracellular Cai2+ (half-maximal inhibitory concentration 52 ± 3.14). Conclusion: In conclusion, the results of this preliminary study demonstrated that Hematite (α-Fe2O3) from B. monosperma flower extract may be a potential therapeutic potential medicament for human breast cancer treatment.


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