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ORIGINAL ARTICLE
Year : 2020  |  Volume : 12  |  Issue : 2  |  Page : 102-106

Inhibitory actions of lupinifolin isolated from Derris reticulata stem against carbohydrate-digesting enzymes


Pharmaceutical Chemistry and Natural Product Research Unit, Faculty of Pharmacy, Mahasarakham University, Maha Sarakham, Thailand

Correspondence Address:
Dr. Pawitra Pulbutr
Pharmaceutical Chemistry and Natural Product Research Unit, Faculty of Pharmacy, Mahasarakham University, Maha Sarakham, 44150
Thailand
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pr.pr_117_19

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Background: Postprandial hyperglycemia is linked with the development of diabetic complications. Inhibition against the carbohydrate-digesting enzymes, specifically pancreatic α-amylase and intestinal α-glucosidase, delays carbohydrate digestion and subsides postprandial plasma glucose (PPG) levels. Objectives: This study aimed to examine the effects of lupinifolin, purified from Derris reticulata stem, a medicinal plant traditionally used for the treatment of diabetes mellitus, on in vitro α-amylase and α-glucosidase enzyme activities and enzyme kinetics. Subjects and Methods: The identification of the isolated phytochemical was performed using nuclear magnetic resonance–mass spectrometry (NMR-MS) spectrometry.In vitro pancreatic α-amylase and α-glucosidase activities and enzyme kinetics were determined using the enzymatic colorimetric methods. Results: The purified phytochemical was identified as lupinifolin from the NMR-MS spectrometry. The isolated lupinifolin produced a significant α-amylase and α-glucosidase inhibitory actions with the median inhibitory concentrations of 3.43 ± 0.77 and 56.29 ± 11.64 μg/mL, respectively. The study of enzyme kinetics showed that lupinifolin exhibited the mode of non-competitive and mixed enzyme inhibitions against α-amylase and α-glucosidase, respectively. Conclusion: The potent α-amylase and α-glucosidase inhibitory actions of lupinifolin derived from D. reticulata stem evidently suggest its potential use as an alternative for the control of PPG.


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