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ORIGINAL ARTICLE
Year : 2020  |  Volume : 12  |  Issue : 1  |  Page : 40-46

Anticancer activity of ethyl acetate extract from lethariella cladonioides in vitro and in vivo


1 School of Public Health, Kunming Medical University, Yunnan Province, 650500, China
2 Yunnan Tumor Institute, The Third Affiliated Hospital of Kunming Medical University, Yunnan Province, 650500, China
3 Physical Examination Center, The Second Affiliated Hospital of Xi'an Medical University, Shanxi Province, 710038, China

Correspondence Address:
Prof. Xiao-Qiong He
School of Public Health, Kunming Medical University, Yunnan Province, 650500
China
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pr.pr_70_19

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Background: Lethariella cladonioides, also known as Red Snow Tea and Golden Silk Tea, mainly grows in Tibet, Sichuan and Yunnan of China. Its medicinal value has been gradually discovered. Objectives: This study mainly evaluated the anti-cancer effect of AMH-L extracted from L. cladonioides in vitro and in vivo and to explore its anticancer mechanism. Materials and Methods: MTT assay was used to determine the cytotoxicity of AMH-L to human cancer cells A-549 and HCT-116 in vitro. Sarcoma S-180 and human lung cancer NCL-H460 cells were implanted into Kunming mice and Balb/C nude mice, respectively, anti-cancer activity in vivo was assessed by recording body weight, organ coefficient and tumor inhibition rate. Flow cytometry and western blotting were used to preliminarily explore the anticancer mechanism. Results: AMH-L, the active component of Red Snow Tea, had strong anticancer activity against A-549 and HCT-116, and IC50was 26.17 ± 3.54 and 16.21 ± 0.83. When the dosage was 50, 100 and 150 mg/kg, the inhibition rates of S-180 in Kunming mice were 36.04%, 43.14%, and 50.45% in turn, respectively. At the end of the experiment, the dose was 150, and the relative proliferation inhibition rate of Balb/C nude mice NCI-H460 tumor was 50.36%. Cell cycle and apoptotic results showed that AMH-L could block A-549 at G2/M phase and promote apoptosis of HCT-116 by inducing poly (ADP-ribose) polymerase lysis. Conclusion: L. cladonioides extract AMH-L exhibits strong anticancer activity in vivo and in vitro and can be used as a potential anticancer drug for further study.


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