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ORIGINAL ARTICLE
Year : 2019  |  Volume : 11  |  Issue : 3  |  Page : 304-309

Study of antidiabetic properties of Uvaria narum leaf extract through glucose uptake and glucose transporter 4 expression studies in 3T3L1 cell line model


1 Department of Chemical and Process Engineering Technology, Jubail Industrial College (JIC), P. O. Box 10099, Jubail Industrial City 31961, Kingdom of Saudi Arabia
2 Department of Biotechnology, Stellixir Biotech Private Limited, Bengaluru, Karnataka, India
3 Sri Padmavati Mahila Visvavidyalayam, Padmavathi Nagar, Near West Railway Station, Tirupati, Chittoor (D.t), Andhra Pradesh, India
4 Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Imam Abdulrahman Bin Faisal University, P. O. Box 2435, Dammam 31441, Kingdom of Saudi Arabia
5 Department of Anatomy, College of Medicine, Imam Abdulrahman Bin Faisal University, P. O. Box 2114, Dammam 31451, Kingdom of Saudi Arabia
6 Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India
7 Department of Medical Biochemistry, College of Applied Medical Sciences - Jubail (CAMSJ), Imam Abdulrahman Bin Faisal University, P. O. Box 4030, Jubail Industrial City, Al Jubail 35816, Kingdom of Saudi Arabia

Correspondence Address:
Dr. Surapaneni Krishna Mohan
Department of Medical Biochemistry, College of Applied Medical Sciences - Jubail, Imam Abdulrahman Bin Faisal University, P.O.Box 4030, Jubail Industrial City, Al Jubail 35816
Kingdom of Saudi Arabia
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pr.pr_7_19

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Background: Uvaria narum (UN) is known to have antipyretic, antimicrobial, anti-inflammatory and antimalarial properties. The antidiabetic properties of UN remains unexplored. The current study has been aimed at understanding the antidiabetic property of UN extract on an in vitro model using 3T3-L1 cell line. Methods: Methanolic extract of UN was prepared, and its cytotoxic effect on 3T3-L1 cells was assessed. Glucose uptake and glucose transporter 4 (GLUT4) translocation in 3T3-L1 cell line on treatment with the extract was evaluated against a standard drug, metformin. α-glucosidase and α-amylase inhibition activities of the extract were also assayed with acarbose as the standard drug. Results: Treatment with UN extract had no cytotoxic effect on the cells. UN extract showed a good percentage inhibition of α-amylase and α-glucosidase activities. UN extract showed 71.31% inhibition and the control drug Acarbose exhibited 88.54% inhibition in α-amylase activity. Furthermore, the extract showed 79.11% inhibition when Acarbose exhibited 87.35% inhibition in α-glucosidase activity. IC50values were also determined. Further, on treatment with the extract, 75.49% of 3T3-L1 cells took up glucose and 70.67% had GLUT4 expression. Conclusion: UN extract enhances glucose uptake and GLUT4 expression, inhibits α-amylase and α-glucosidase activities, thereby demonstrating the antidiabetic properties in vitro.


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