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ORIGINAL ARTICLE
Year : 2019  |  Volume : 11  |  Issue : 3  |  Page : 244-247

Phytochemical content and antioxidant activities of pomelo peel extract


1 Department of Applied Pharmaceutical Science and Clinical Pharmacy, Faculty of Pharmacy, Al-Isra University, Amman, Jordan
2 Department of Pharmacy, Al-Rasheed University College, Baghdad, Iraq
3 Department of Pharmacy, Al-Mustaqbal University College, Babylon, Iraq

Correspondence Address:
Dr. Zead Helmi Abudayeh
Department of Applied Pharmaceutical Science and Clinical Pharmacy, Faculty of Pharmacy, Al-Isra University, Amman
Jordan
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pr.pr_180_18

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Background: There is an increasing interest in the antioxidant measurement of some plant constituents. Plant component, especially fruit, has antioxidant components which are safe, economic, and powerful to reduce oxidative stress and replace synthetic ones. Objective: To quantitatively measure the flavonoids, Vitamin C, and carotenoid content of hydroalcoholic extract and to estimate the antioxidant activity of pomelo peel extract (PPE) and minimum inhibitory concentration 50% (IC50) of the extract using 2,2-diphenyl-1-picrylhydrazyl (DPPH) ethanolic solution. Materials and Methods: Hydroalcoholic PPE was prepared; total flavonoids using aluminum chloride method, Vitamin C using thiourea and 2,4-dinitrophenylhydrazine method, and carotenoid content of the extract were estimated using 95% ethanol solution. All measurements were estimated using ultraviolet–visible spectrophotometer. Antioxidant activity and IC50of PPE were measured using DPPH method. Results: Flavonoids, Vitamin C, and carotenoid content were measured as 21.20 mg/100 g, 15.13 mg/100 g, and 62.98 μg/g, respectively, of dry PPE. The DPPH radical scavenging activity exhibits a significant dose-dependent inhibition of DPPH radical color, with IC50being at a concentration of 68.55 μg/ml of the extract, comparable to 55.87 μg/ml IC50of Vitamin C standard antioxidant. Conclusion: From the study results, we could conclude that pomelo peel possesses high antioxidant properties and it is potentially rich sources of natural antioxidants.


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