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ORIGINAL ARTICLE
Year : 2019  |  Volume : 11  |  Issue : 3  |  Page : 224-229

Evaluation of phytochemical content of white tea clone 100 and changes the expression of tumor suppressor genes on colorectal cancer cell line HCT116


1 Department of Biotechnology and Plant Breeding, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Department of Clinical Genetic, National Institute of Genetic Engineering and Biotechnology, Karaj, Iran
3 Department of Seed and Plant Improvement, Tea Research Institute, Guilan 44159-77555, Iran

Correspondence Address:
Dr. Asa Ebrahimi
Department of Biotechnology, Faculty of Agricultural Sciences and Food Industries, Science and Research Branch, Islamic Azad University, Ashrafi Esfahani Highway, Tehran 14778-93855
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pr.pr_39_19

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Aim/Background: Colorectal carcinoma cancer is one of the main types of cancer with high death rate of patients, according to their non-healthy lifestyle. In this research, we evaluated inhibitory of white tea clone 100 extract on colorectal cancer cell line HCT-116, and its effectiveness in expression level of three tumor suppressor genes. Materials and Methods: Total polyphenolic content in all samples were measured using the Folin–Ciocalteu method, and free radical adsorption investigated by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to evaluate the white tea extract inhibitory effect on HCT-116 cells. Results: Among different extraction methods, the white tea aqueous extracts produced the highest amount of polyphenols and DPPH radical scavenging activity (36.67 ± 0.54 mg gallic acid equivalent/g dry weight and 71.74% ± 0.42%, respectively). Cell survival analysis in the MTT assay indicated that aqueous white tea extract could reduce the viability of HCT-116 cells in 8, 16, and 24 h considerably, related to the concentration-dependent manner. The real-time polymerase chain reaction results indicated the significantly increased expression level (P < 0.05) of tumor suppressor genes DCC , TGFBR2 , and P53 in the concentration of 1000 mg/ml at 24 h. Conclusion: White tea aqueous extract because of having hydroxyl group in its structure at optimal concentration in all three times of experimental, could lead to a positive changes effect on gene expression in the tumor suppressor family. Key words: 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, HCT-116 cell line, radical scavenging activity, tumor suppressor genes, white tea


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