|Year : 2019 | Volume
| Issue : 1 | Page : 98-101
Suppression of polyps formation by saffron extract in Adenomatous polyposis coliMin/+ mice
Kyoko Fujimoto1, Tomoe Ohta2, Hitomi Yamaguchi3, Nguyen Huu Tung4, Gen Fujii5, Michihiro Mutoh6, Takuhiro Uto2, Yukihiro Shoyama2
1 Department of Biochemistry, Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, Nagasaki, Japan
2 Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, Nagasaki, Japan
3 Department of Molecular Biology, Faculty of Pharmaceutical Sciences, Nagasaki International University, Sasebo, Nagasaki, Japan
4 School of Medicine and Pharmacy, Vietnam National University, Hanoi, Vietnam
5 Central Radioisotope Division, Research Institute, Tokyo, Japan
6 Epidemiology and Prevention Division, Research Center for Cancer Prevention and Screening, National Cancer Center, Tokyo, Japan
|Date of Web Publication||20-Feb-2019|
Prof. Yukihiro Shoyama
Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagasaki International University, 2825-7 Huis Ten Bosch, Sasebo, Nagasaki 859-3298
Source of Support: None, Conflict of Interest: None
| Abstract|| |
Saffron (Crocus sativus L.) has been used both as a food additive for flavoring and coloring and in traditional medicine. Saffron extract and its main component crocin decrease the growth of several types of human cancer, including colorectal cancer in vitro. Numerous polyps develop in the small intestine in the Adenomatous polyposis coli (Apc) deficiency mice. ApcMin/+ mice are models for human familial adenomatous polyposis and human colon cancer patients. In this study, we examined the efficacy of saffron extract added to diet on reducing the polyp density in ApcMin/+ mice. ApcMin/+ mice were either given a placebo or saffron extract (0.1% and 0.5%) diet for 4 weeks. At 12 weeks of age, intestines were analyzed for polyp number in the small intestine. Our analysis confirmed that crocin (1), crocin-2 (2), and crocin-4 (4) are the major compounds in the saffron extract and the content of 1 in the tested saffron extract was 29.2%. Saffron extract decreased the number of intestinal polyps in a concentration-dependent manner in ApcMin/+ mice. Notably, the number of polyps in the distal small intestine of the mice fed with 0.5% saffron extract was significantly decreased compared with the placebo. These results indicate that saffron extract can reduce the polyp number in the ApcMin/+ mice.
Abbreviations Used: FAP: Familial adenomatous polyposis; Apc: Adenomatous polyposis coli.
Keywords: Adenomatous polyposis coliMin/+ mice, crocin, Crocus sativus, intestinal polyps, saffron
|How to cite this article:|
Fujimoto K, Ohta T, Yamaguchi H, Tung NH, Fujii G, Mutoh M, Uto T, Shoyama Y. Suppression of polyps formation by saffron extract in Adenomatous polyposis coliMin/+ mice. Phcog Res 2019;11:98-101
|How to cite this URL:|
Fujimoto K, Ohta T, Yamaguchi H, Tung NH, Fujii G, Mutoh M, Uto T, Shoyama Y. Suppression of polyps formation by saffron extract in Adenomatous polyposis coliMin/+ mice. Phcog Res [serial online] 2019 [cited 2020 Sep 28];11:98-101. Available from: http://www.phcogres.com/text.asp?2019/11/1/98/252565
- Saffron has been used both as a food additive and in traditional medicine
- Adenomatous polyposis coli (Apc) Min/+ mouse is a model of familial adenomatous polyposis (FAP)
- We investigated the effect of the saffron extract on polyp formation using ApcMin/+ mice
- Saffron extract suppressed the number of intestinal polyps in ApcMin/+ mice
- Saffron extract might be effective in preventing intestinal polyp formation for FAP patients.
| Introduction|| |
Crocus sativus L. is a perennial herb belonging to the iris family (Iridaceae). When dried, it is commonly called “Saffron” and is used both as a food additive for flavoring and coloring and as a drug in medicine.,, Saffron is cultivated worldwide, especially in Iran, India, Greece, Morocco, Spain, and China. Phytochemical research reported that the main component of saffron is crocin [Figure 1], an ester glycoside of crocetin. Other typical components such as picrocrocin and safranal, related to the flavor of the herb, have been isolated from saffron.,
|Figure 1: The chemical structures of crocetin glycosides (1–4) and crocetin (5)|
Click here to view
Pharmacological studies have revealed that saffron extracts and/or the active constituents have properties to improve learning and memory and have anticonvulsant, antidepressant and anti-inflammatory properties.,,,, Free radical scavenging, antioxidant activity, and promotion of the diffusion of oxygen in different tissues were also reported to be the properties of saffron extracts or their bioactive constituents., Other biological effects of saffron and its constituents include the induction of apoptosis, antihyperlipidemic effects, immunomodulation, and antineurodegenerative effects.,,,, Our previous studies have demonstrated several bioactivities of saffron and/or crocin. Crocin promotes nonrapid eye movement sleep in mice. Saffron and crocin exhibit neuroprotective activities in vivo and in vitro.,,,, In addition, we prepared monoclonal antibodies against crocin.
Several studies have revealed the effects of saffron and its ingredients on carcinogenesis., Saffron extract and crocin were reported to inhibit the growth of several types of human cancer cells.,, We also indicated the prevention of skin tumor formation in mice and a decrease in the proliferation of human colorectal cancer cells., However, the effects of saffron on intestinal carcinogenesis in vivo have not been clarified yet.
Penetrant dominant mutation of Adenomatous polyposis coli (Apc) is known to lead the numerous intestinal polyp development., Familial adenomatous polyposis (FAP) is the hereditary disease that develops hundreds of intestinal polyps. In this disease, intestinal polyps develop from young generation and increase the risk of transformation that leads to colorectal cancer development. The ApcMin/+ mouse is a model of FAP that is lacking a functional Apc gene product. By using this well-recognized mouse model, we examined the effect of the saffron extract on intestinal polyps in vivo for the first time whether saffron extract can inhibit the development of intestinal polyps.
| Materials and Methods|| |
The stigmas of C. sativus (saffron) were collected in Oita Prefecture, Japan, in 2010 and were authenticated by one of the authors (Y. S.). A voucher specimen was deposited in the Department of Pharmacognosy, Nagasaki International University, Japan.
Preparation of saffron aqueous extract for in vivo study
The air-dried and shade-dried saffron (500 g) was pulverized and then extracted with 50% aqueous EtOH (2.0 l × 3 times) at 40°C under sonication. The combined extracts were concentrated into dark brown syrup (280 g).
High-performance liquid chromatography analysis
The pure main pigment crocetin glycosides, crocin (1), crocin-2 (2), crocin-3 (3), and crocin-4 (4), were dissolved in MeOH at a concentration of 1.0 mg/ml and the saffron extract (10.0 mg) was prepared in MeOH (10 mg/ml). All samples were filtered with 0.45-μm syringe filters and stored at −20°C until use. Peaks in the saffron extract were unambiguously assigned by comparison of their retention time with those of authentic specimens. [Figure 1] shows the chemical structures of crocetin (5) and crocetin glycosides (1–4).
For the quantitative analysis of 1, various concentrations were prepared to determine the calibration curve. The calibration curve was constructed using the content versus peak area (y = 28.082x + 28.563, R2 = 0.9998, linear range: 0.015–0.25 mg/ml). The content of 1 in the saffron extract was calculated using the standard curve.
High-performance liquid chromatography instruments and conditions
The high-performance liquid chromatography tests were performed on a TOSOH 8020 series (Tosoh, Tokyo, Japan) equipped with an intelligent ultraviolet (UV)/visible detector (UV-8020), two dual pump (DV-8020), and a degasser (SD-8020). The chromatographic separation was performed on a TSK Gel ODS-100V (4.6 mm × 250 mm with 5-μm particle size; Tosoh Co., Tokyo, Japan) in the following conditions: mobile phase A (MeOH) and B (H2O containing 1.0% acetic acid), gradient program, 0–5 min (30:70, v/v), 5–20 min (30:70 → 50:50, v/v), 20–30 min (50:50 → 70:30, v/v), and 30–45 min (70:30 → 100:0, v/v). The flow rate was 1.0 ml/min, the injection volume was 10.0 μl, the detection was performed at 442 nm, and the program was held at ambient temperature throughout the analysis.
Mice and diet
The ApcMin/+ (C57BL/6J) mice were supplied by Jackson Laboratories (Bar Harbor, Maine, USA). The mice were maintained under specific pathogen-free conditions and 12:12 light/dark cycles at the Animal Center of Nagasaki International University (Nagasaki, Japan). In this research, we analyzed 30 mice in total (16 females, 14 males). Four weeks after birth, all baby mice were genotyped from a tail sample. The examined ApcMin/+ genotype mice were separated in three groups, including two test groups and one control group. The mouse diet was compounded with two different doses of saffron extract, 0.1% and 0.5% (i.e., 0.25 and 1.25 g/kg body weight/day, respectively). The control mice were not given any saffron extract. The two test groups (from 8 weeks old) were given food mixed with the saffron extract for 4 weeks. According to standard dietary intake guidelines provided by the Ministry of Health, Labour and Welfare, we calculated the appropriate dose for the mice. Mice were sacrificed at 12 weeks of age for the analysis of intestinal polyps. All animal experiments were conducted according to the Guidelines for Animal Experiments from the Faculty of Pharmaceutical Sciences, Nagasaki International University (approval number 124).
Mouse tails were genotyped using a KAPA Express Extract (NIPPON Genetics Co., Ltd., Japan) according to the manufacturer's protocol. Allele-specific polymerase chain reaction primers for the Apc gene were produced according to genotyping protocols of the Jackson Laboratory (https://www2.jax.org/protocolsdb/f?p=116:5:0:NO:5:P5_MASTER_PROTOCOL_ID, P5_JRS_CODE:21922,002020).
Counts of intestinal polyps and statistical analysis
After the 12-week-old mice were sacrificed, their small intestines were removed and divided into three equal segments. These segments were incised longitudinally and then washed with chilled phosphate-buffered saline. The washed intestines were laid flat on filter paper and fixed in 10% neutral-buffered formalin. The number of intestinal polyps was counted under light microscopy. All statistical analyses were carried out using the GraphPad Prism 5 program (GraphPad Software Inc., San Diego, CA). Statistical analysis of the number of polyps was performed by Dunnett's multiple comparison test.
| Results and Discussion|| |
There are several arguments regarding absorption of glycosides and one of them is the direct absorption and the other hydrolysis then absorbs. It has not been evident in the case of crocin., However, it is clear that crocin and/or crocetin can be incorporated into cell since previously we prepared anti-crocin monoclonal antibody which has affinity against both components and confirmed by immunostaining of cell.,
The polyp number was significantly decreased in Adenomatous polyposis coliMin/+ mice
The average number of polyps in the small intestine of mice fed with saffron extract at doses of 0% (placebo), 0.1% and 0.5% was 143.6 ± 19.3, 133.4 ± 15.3, and 104.3 ± 16.0, respectively. The difference in polyp number was statistically significant compared the mice fed with 0.5% saffron extract to that of placebo (P < 0.05, Dunnett's multiple comparison test) [Table 1]. The number of the polyp was most decreased in a distal part that located next to the large intestine (0.5% of saffron extract versus placebo, P = 0.023). The number of intestinal polyps in the proximal and middle part was decreasing but failed to obtain the significant difference.
|Table 1: The average number of polyps in the small intestine and large intestine of mice fed with saffron extract|
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Interestingly, we found that the distal part of the small intestine seems to display the highest sensitivity to the saffron extract. We assumed that the underlying reason might be due to the longer residence time of intestinal contents in the distal part of the small intestine than the proximal part. As is exposed longer to the saffron extract, as the effects of saffron may be remarkable. A time-dependent effect of saffron extract could be evident in the in vitro experiment.
The content of crocin in saffron extract
A chromatogram of crocin (1) is shown in [Figure 2]a. The retention time and purity of crocin was 29.6 min and 95.7% (relative pic area), respectively.
|Figure 2: (a) A high-performance liquid chromatography chromatogram for standard crocin (1) (concentration 1.5 mg/ml). (b) A high-performance liquid chromatography chromatogram for saffron extract (concentration 2.5 mg/ml). Number to peak identity; crocin (1), crocin-2 (2), crocin-3 (3), and crocin-4 (4)|
Click here to view
[Figure 2]b shows the chromatogram of the saffron extract. As illustrated, crocin (1), crocin-2 (2), and crocin-4 (4) are the major compounds in the saffron extract, and they were clearly separated from each other. Because 1 has been used as the compound for the quality control of saffron products, the content of 1 was examined quantitatively. The amount of 1 in the tested saffron extract was 29.2%.
In this study, the intake amount of saffron extract was that could be able to intake in daily life. Intake of 0.1% and 0.5% of the saffron extract was calculated based on the dietary fiber reference intakes for Japanese determined by the Ministry of Health, Labour and Welfare (approximately 300 mg/kg/day). As 29.2% of the saffron extract was crocin, we calculated that the content of the crocin in 0.1% and 0.5% of saffron extract could be 0.073 g/kg/day and 0.365 g/kg/day.
| Conclusion|| |
This is the first report using ApcMin/+ mice to examine the effect of saffron extract on polyp formation. We assume that saffron extract might be effective in preventing intestinal polyp formation for FAP patients. As is already used in medicine, further experiments, especially for its safety evaluation is needed. Furthermore, we found that saffron and/or crocin can prevent the colon cancer by inhibition of colitis-associated colorectal carcinogenesis because the anti-inflammatory effects of crocin are suggested to be based on its strong antioxidant activity rather than that of alpha-tocopherol., Therefore, we have reached to further the ability of cancer preventive activity of crocin because in this investigation we have found the inhibitory activity against polyp formation which will be transformed and lead to colon cancer.
Financial support and sponsorship
Conflicts of interest
There are no conflicts of interest.
| References|| |
Bathaie SZ, Mousavi SZ. New applications and mechanisms of action of saffron and its important ingredients. Crit Rev Food Sci Nutr 2010;50:761-86.
Gantait S, Vahedi M.In vitro
regeneration of high value spice Crocus sativus
L.: A concise appraisal. J Appl Res Med Aromat Plants 2015;2:124-33.
Schmidt M, Betti G, Hensel A. Saffron in phytotherapy: Pharmacology and clinical uses. Wien Med Wochenschr 2007;157:315-9.
Kabiri M, Rezadoost H, Ghassempour A. A comparative quality study of saffron constituents through HPLC and HPTLC methods followed by isolation of crocins and picrocrocin. LWT Food Sci Technol 2017;84:1-9.
Melnyk JP, Wang S, Marcone MF. Chemical and biological properties of the world's most expensive spice: Saffron. Food Res Int 2010;43:1981-9.
Papandreou MA, Tsachaki M, Efthimiopoulos S, Cordopatis P, Lamari FN, Margarity M, et al.
Memory enhancing effects of saffron in aged mice are correlated with antioxidant protection. Behav Brain Res 2011;219:197-204.
Hosseinzadeh H, Talebzadeh F. Anticonvulsant evaluation of safranal and crocin from Crocus sativus
in mice. Fitoterapia 2005;76:722-4.
Akhondzadeh S, Fallah-Pour H, Afkham K, Jamshidi AH, Khalighi-Cigaroudi F. Comparison of Crocus sativus
L. and imipramine in the treatment of mild to moderate depression: A pilot double-blind randomized trial [ISRCTN45683816]. BMC Complement Altern Med 2004;4:12.
Hosseinzadeh H, Younesi HM. Antinociceptive and anti-inflammatory effects of Crocus sativus
L. stigma and petal extracts in mice. BMC Pharmacol 2002;2:7.
Menghini L, Leporini L, Vecchiotti G, Locatelli M, Carradori S, Ferrante C, et al. Crocus sativus
L. Stigmas and byproducts: Qualitative fingerprint, antioxidant potentials and enzyme inhibitory activities. Food Res Int 2018;109:91-8.
Carlsen MH, Halvorsen BL, Holte K, Bøhn SK, Dragland S, Sampson L, et al.
The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide. Nutr J 2010;9:3.
El-Beshbishy HA, Hassan MH, Aly HA, Doghish AS, Alghaithy AA. Crocin “saffron” protects against beryllium chloride toxicity in rats through diminution of oxidative stress and enhancing gene expression of antioxidant enzymes. Ecotoxicol Environ Saf 2012;83:47-54.
Bakshi H, Sam S, Rozati R, Sultan P, Islam T, Rathore B, et al.
DNA fragmentation and cell cycle arrest: A hallmark of apoptosis induced by crocin from Kashmiri saffron in a human pancreatic cancer cell line. Asian Pac J Cancer Prev 2010;11:675-9.
Lee IA, Lee JH, Baek NI, Kim DH. Antihyperlipidemic effect of crocin isolated from the fructus of Gardenia jasminoides
and its metabolite crocetin. Biol Pharm Bull 2005;28:2106-10.
Bani S, Pandey A, Agnihotri VK, Pathania V, Singh B. Selective Th2 upregulation by Crocus sativus
: A neutraceutical spice. Evid Based Complement Alternat Med 2011;2011. pii: 639862.
Geromichalos GD, Lamari FN, Papandreou MA, Trafalis DT, Margarity M, Papageorgiou A, et al.
Saffron as a source of novel acetylcholinesterase inhibitors: Molecular docking and in vitro
enzymatic studies. J Agric Food Chem 2012;60:6131-8.
Howes MJ, Perry E. The role of phytochemicals in the treatment and prevention of dementia. Drugs Aging 2011;28:439-68.
Masaki M, Aritake K, Tanaka H, Shoyama Y, Huang ZL, Urade Y, et al.
Crocin promotes non-rapid eye movement sleep in mice. Mol Nutr Food Res 2012;56:304-8.
Soeda S, Aritake K, Urade Y, Sato H, Shoyama Y. Neuroprotective activities of saffron and crocin. Adv Neurobiol 2016;12:275-92.
Ochiai T, Shimeno H, Mishima K, Iwasaki K, Fujiwara M, Tanaka H, et al.
Protective effects of carotenoids from saffron on neuronal injury in vitro
and in vivo
. Biochim Biophys Acta 2007;1770:578-84.
Ochiai T, Ohno S, Soeda S, Tanaka H, Shoyama Y, Shimeno H, et al.
Crocin prevents the death of rat pheochromyctoma (PC-12) cells by its antioxidant effects stronger than those of alpha-tocopherol. Neurosci Lett 2004;362:61-4.
Soeda S, Ochiai T, Paopong L, Tanaka H, Shoyama Y, Shimeno H, et al.
Crocin suppresses tumor necrosis factor-alpha-induced cell death of neuronally differentiated PC-12 cells. Life Sci 2001;69:2887-98.
Ochiai T, Soeda S, Ohno S, Tanaka H, Shoyama Y, Shimeno H, et al.
Crocin prevents the death of PC-12 cells through sphingomyelinase-ceramide signaling by increasing glutathione synthesis. Neurochem Int 2004;44:321-30.
Xuan L, Tanaka H, Xu Y, Shoyama Y. Preparation of monoclonal antibody against crocin and its characterization. Cytotechnology 1999;29:65-70.
Abdullaev FI, Espinosa-Aguirre JJ. Biomedical properties of saffron and its potential use in cancer therapy and chemoprevention trials. Cancer Detect Prev 2004;28:426-32.
Abdullaev FI. Cancer chemopreventive and tumoricidal properties of saffron (Crocus sativus
L.). Exp Biol Med (Maywood) 2002;227:20-5.
Noureini SK, Wink M. Antiproliferative effects of crocin in HepG2 cells by telomerase inhibition and hTERT down-regulation. Asian Pac J Cancer Prev 2012;13:2305-9.
Chryssanthi DG, Lamari FN, Iatrou G, Pylara A, Karamanos NK, Cordopatis P, et al.
Inhibition of breast cancer cell proliferation by style constituents of different Crocus
species. Anticancer Res 2007;27:357-62.
Bakshi HA, Sam S, Feroz A, Ravesh Z, Shah GA, Sharma M, et al.
Crocin from Kashmiri saffron (Crocus sativus
) induces in vitro
and in vivo
xenograft growth inhibition of Dalton's lymphoma (DLA) in mice. Asian Pac J Cancer Prev 2009;10:887-90.
Konoshima T, Takasaki M, Tokuda H, Morimoto S, Tanaka H, Kawata E, et al
. Crocin and crocetin devrivatives inhibit skin tumor promotion in mice. Phytotherapy Res 1998;12:400-4.
Aung HH, Wang CZ, Ni M, Fishbein A, Mehendale SR, Xie JT, et al.
Crocin from Crocus sativus
possesses significant anti-proliferation effects on human colorectal cancer cells. Exp Oncol 2007;29:175-80.
Moser AR, Pitot HC, Dove WF. A dominant mutation that predisposes to multiple intestinal neoplasia in the mouse. Science 1990;247:322-4.
Moser AR, Dove WF, Roth KA, Gordon JI. The min (multiple intestinal neoplasia) mutation: Its effect on gut epithelial cell differentiation and interaction with a modifier system. J Cell Biol 1992;116:1517-26.
Vogelstein B, Fearon ER, Hamilton SR, Kern SE, Preisinger AC, Leppert M, et al.
Genetic alterations during colorectal-tumor development. N Engl J Med 1988;319:525-32.
Pan W, Xue B, Yang C, Miao L, Zhou L, Chen Q, et al.
Biopharmaceutical characters and bioavailability improving strategies of ginsenosides. Fitoterapia 2018;129:272-82.
Hostetler GL, Ralston RA, Schwartz SJ. Flavones: Food sources, bioavailability, metabolism, and bioactivity. Adv Nutr 2017;8:423-35.
Hammer J, Pruckmayer M, Bergmann H, Kletter K, Gangl A. The distal colon provides reserve storage capacity during colonic fluid overload. Gut 1997;41:658-63.
Kawabata K, Tung NH, Shoyama Y, Sugie S, Mori T, Tanaka T, et al.
Dietary crocin inhibits colitis and colitis-associated colorectal carcinogenesis in male ICR mice. Evid Based Complement Alternat Med 2012;2012:820415.
[Figure 1], [Figure 2]