Home | About PR | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |   Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2018  |  Volume : 10  |  Issue : 4  |  Page : 422-425

Validation of high-performance liquid chromatography method for quantification of costunolide content in Laurus nobilis


1 Analytical Research and Development, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, India
2 Phytochemistry Lab, Vidya Herbs Pvt. Ltd, Bangalore, Karnataka, India

Correspondence Address:
Dr. Puttaswamy Naveen
Analytical Research and Development, Vidya Herbs Pvt. Ltd, Jigani, Anekal Taluk, Bengaluru, Karnataka
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pr.pr_31_18

Rights and Permissions

Objective: To establish a method to determine the content of costunolide in Laurus nobilis leaves by high-performance liquid chromatography (HPLC). Materials and Methods: The separation was performed on a reversed-phase C18 column (100 Å, 150 mm × 4.6 mm, 5 μm pore size) using a mobile phase composed of water:acetonitrile (40:60) at a flow rate of 1.0 ml/min. The detection was carried out on a ultraviolet detector at 210 nm. The developed method was validated according to the requirements for International Conference on Harmonisation guidelines. Results: The proposed method for costunolide was validated for linearity with excellent correlation coefficient (r2 > 0.999). The relative standard deviation (RSD) is less than 1% in precision (i.e repeatability and intermediate) of the method. The recovery rate for costunolide was within 100.54%–102.62%. The limit of detection and limit of quantitation were 2.29 and 6.64 parts per million, respectively. Conclusion: The developed HPLC method is simple, rapid, precisely, accurately, and widely accepted and it is recommended for efficient assays in routine work. Abbreviation Used: HPLC: High-performance liquid chromatography; UV: Ultraviolet; ICH: International Conference on Harmonisation; r2: Correlation coefficient; WHO: World Health Organization; ppm: Parts per million; % RSD: Percentage relative standard deviation


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed123    
    Printed5    
    Emailed0    
    PDF Downloaded5    
    Comments [Add]    

Recommend this journal