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ORIGINAL ARTICLE
Year : 2018  |  Volume : 10  |  Issue : 1  |  Page : 98-103

Evaluation of Antithrombotic Activities of Solanum xanthocarpum and Tinospora cordifolia


Department of Microbiology and Cell Biology, Indian Institute of Science, Bengaluru, Karnataka, India

Correspondence Address:
Dr. William R Surin
Department of Microbiology and Cell Biology, Indian Institute of Science, Bengaluru - 560 012, Karnataka
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/pr.pr_80_17

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Background: Solanum xanthocarpum and Tinospora cordifolia have been reported to exhibit anti-inflammatory, antiarthritic, antioxidant, antiallergic, and hepatoprotective activities. The origins of many of the currently available antithrombotic treatments are from natural products and natural sources. Objective: To investigate the antithrombotic activities of methanolic leaf extracts of S. xanthocarpum(SXME) and T. cordifolia(TCME). Materials and Methods: Antithrombotic activities were assessed by thrombin inhibition assay, thrombin generation assay, platelet adhesion assay on collagen-coated surface, and platelet PAC1-FITC binding by flow cytometry. Results: SXME significantly inhibited thrombin activity at 5–20 mg/ml concentrations, whereas TCME inhibited thrombin activity at 500 μg/ml–5 mg/ml concentrations. Further, SXME inhibited thrombin generation at 2–20 mg/ml concentrations, whereas TCME exhibited significant inhibition at 200 μg/ml, suggesting that TCME has higher efficacy as compared to SXME. Moreover, SXME did not inhibit platelet adhesion on collagen-coated surface, whereas TCME inhibited platelet adhesion on collagen-coated surface at 5 mg/ml. Indomethacin showed significant inhibition in platelet adhesion at 300 μM. Further, SXME inhibited thrombin-induced platelet activation (PAC1-FITC binding) significantly at 1 mg/ml by about 80%, whereas TCME inhibited thrombin-induced platelet activation (PAC1-FITC binding) by about 40% at 1 mg/ml. Conclusion: These results strongly suggested that SXME and TCME possess antithrombotic activities. However, further studies are essential to find out the active constituent responsible for antithrombotic effect.


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