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ORIGINAL ARTICLE
Year : 2017  |  Volume : 9  |  Issue : 1  |  Page : 96-100

Antifungal activity of hydroalcoholic extract of Chrysobalanus icaco against oral clinical isolates of Candida Species


1 Graduate Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará, Belém, Pará, Brazil
2 Pharmaceutical Innovation Graduate Program, Institute of Health Sciences, Federal University of Pará, Belém, Pará, Brazil
3 Graduate Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Pará; Pharmaceutical Innovation Graduate Program, Institute of Health Sciences, Federal University of Pará, Belém, Pará, Brazil

Correspondence Address:
Marcieni Ataíde Andrade
Graduate Program in Pharmaceutical Sciences, Institute of Health Sciences, Federal University of Para - Augusto Correa Street, Number 01, Postal Code 66075 110, Belem, Para
Brazil
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-8490.199772

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Background: Chrysobalanus icaco is a medicinal plant commonly used to treat fungal infections in Brazilian Amazonian region. Objective: This work aimed to evaluate the antifungal activity of the hydroalcoholic extract of C. icaco (HECi) against oral clinical isolates of Candida spp. and to determine the pharmacognostic parameters of the herbal drug and the phytochemical characteristics of HECi. Materials and Methods: The pharmacognostic characterization was performed using pharmacopoeial techniques. Phytochemical screening, total flavonoid content, and high-performance liquid chromatography (HPLC) analysis were used to investigate the chemical composition of the HECi. A broth microdilution method was used to determine the antifungal activity of the extract against 11 oral clinical isolates of Candida spp. Results: Herbal drug presented parameters which were within the limits set forth in current Brazilian legislation. A high amount of flavonoid content (132,959.33 ± 12,598.23 μg quercetin equivalent/g of extract) was found in HECi. Flavonoids such as myricetin and rutin were detected in the extract by HPLC analyses. HECi showed antifungal activity against oral isolates of Candida albicans and Candida parapsilosis (minimum inhibitory concentrations [MIC] 3.12 and 6.25 mg/mL, respectively), and C. albicans American American Type Culture Collection (MIC <1.56 mg/mL). Conclusion: HECi was shown to possess antifungal activity against Candida species with clinical importance in the development of oral candidiasis, and these activities may be related to its chemical composition. The antifungal activity detected for C. icaco against Candida species with clinical importance in the development of oral candidiasis can be attributed to the presence of flavonoids in HECi, characterized by chromatographic and spectroscopic techniques.


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