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ORIGINAL ARTICLE
Year : 2017  |  Volume : 9  |  Issue : 1  |  Page : 87-95

The hydroalcoholic extract of Matricaria chamomilla suppresses migration and invasion of human breast cancer MDA-MB-468 and MCF-7 cell lines


1 Department of Biochemistry, Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
2 Student Research Committee, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
3 Pharmaceutics Research Center, Institute of Neuropharmacology; Department of Toxicology and Pharmacology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
4 Department of Anatomy and Embryology, Cellular and Molecular Research Center, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
5 Department of Nutrition, Social Determinants of Health Research Center, Faculty of Health, Yasuj University of Medical Sciences, Yasuj, Iran
6 Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Correspondence Address:
Reza Mahmoudi
Department of Anatomy and Embryology, Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj
Iran
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-8490.199778

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Background: Matricaria chamomilla is an aromatic plant with antioxidant, anticancer, and anti-inflammatory properties. However, the inhibitory role of M. chamomilla on migration and invasion of human breast cancer cells remains unclear. Objective: This study investigated the methods to evaluate these anticancer mechanisms of M. chamomilla on human breast cancer MCF-7 and MDA-MB-468 cell lines. Materials and Methods: The cells were treated with hydroalcoholic extract of M. chamomilla at different concentrations (50–1300 μg/mL) for 24, 48, and 72 h in a culture medium containing 10% fetal bovine serum. This study quantified the 50% growth inhibition concentrations (IC50) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay; apoptosis and necrosis through Hoechst 33342/propidium iodide staining; cell proliferation and clone formation by clonogenic assay as well as cellular migration, invasion, and attachment. After 24, 48, and 72 h of treatment, the IC50levels were 992 ± 2.3 μg/mL, 893 ± 5.4 μg/mL, and 785 ± 4.8 μg/mL against MDA-MB-468, respectively, and 1288 ± 5.6 μg/mL, 926 ± 2.5 μg/mL, and 921 ± 3.5 μg/mL, against MCF-7, respectively. Furthermore, increasing the extract concentrations induced cellular apoptosis and necrosis and decreased cellular invasion or migration through 8 μm pores, colonization and attachment in a dose-dependent manner. Results: It indicated time- and dose-dependent anti-invasive and antimigrative or proliferative and antitoxic effects of hydroalcoholic extract of aerial parts of chamomile on breast cancer cells. Conclusion: This study demonstrated an effective plant in preventing or treating breast cancer.


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