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ORIGINAL ARTICLE
Year : 2016  |  Volume : 8  |  Issue : 5  |  Page : 56-60

Extracts from Flammulina velutipes inhibit the adhesion of pathogenic fungi to epithelial cells


1 Department of Biology, Division of Natural and Exact Sciences, Campus Guanajuato, University of Guanajuato, Noria Alta S/N, Col. Noria Alta, Guanajuato, Guanajuato 36050, Mexico
2 Department of Science and Engineering, Campus León, University of Guanajuato, Loma del Bosque 103 Col, Lomas del Campestre, León, Guanajuato 37150, Mexico
3 Department of Medical Sciences, Division of Health Sciences, Campus León, University of Guanajuato, 20 de Enero 292, Col. Obregón, León, Guanajuato 37320, Mexico
4 Department of Infectomics and Molecular Pathogenesis, Center for Research and Advanced Studies, National Polytechnic Institute (CINVESTAV-IPN), Av. Instituto Politécnico Nacional 2508, San Pedro Zacatenco, Gustavo A, Madero, 07360, City, Mexico

Correspondence Address:
Myrna Sabanero Lopez
Department of Biology, Division of Natural and Exact Sciences, Campus Guanajuato, University of Guanajuato, Noria Alta S/N, Col. Noria Alta
Mexico
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-8490.178648

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Background: Recently, extracts from natural sources have been tested for their antifungal properties. In this aspect, Flammulina velutipes extracts possess a significant amount of branch-chained carbohydrates with mannose moieties that, hypothetically, can reduce the adhesion. Objective: In this study, we assessed the capacity of extracts from F. velutipes (wild-type AQF-1 and ATCC 34574 as the reference strain) to inhibit the adhesion of S. schenkii and C. albicans to epithelial cells. Materials and Methods: The aqueous extracts from F. velutipes strains were obtained by sonication, total carbohydrate and protein was analyzed by Dubois and Lowry methods respectively. Effect of the extracts (50, 100 and 150 μg/mL) on the fungi adhesion to host cells was evaluated after 1 h interaction, and the percentage of inhibition of adhesion was measured. After of interaction the cytoskeleton from cell was analyzed with phalloidin-FITC. Results: The extract from strain AQF-1 (50, 100 and 150 μg/mL) inhibited the adhesion of: S. schenkii in a dose-dependent manner (4.9, 7.5 and 12.7%, respectively) and C. albicans in a dose-independent manner (5.2%). The percentage of inhibition by extracts from the strain ATCC34574 at the same concentrations, shown that are dose independent for both fungi: 3.9% for S. schenkii and 2.6% for C. albicans. Conclusion: The extracts from F. velutipes inhibit the adhesion of pathogenic fungi to host cells. The mechanism molecular is unknown; however, is probably an interaction between the polysaccharides from extracts with the fungi receptors. This aspect is currently analyzed.


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