Home | About PR | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |   Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
ORIGINAL ARTICLE
Year : 2016  |  Volume : 8  |  Issue : 4  |  Page : 253-257

Chemical characterization and cytoprotective effect of the hydroethanol extract from Annona coriacea Mart. (Araticum)


1 Department of Biotechnology, Ceará State University, Fortaleza (CE), Brazil
2 Department of Biological Chemistry, Regional University of Cariri, Crato (CE), Brazil
3 Department of Biomedicine, Leão Sampaio College, Juazeiro do Norte (CE), Brazil
4 Department of Medical Microbiology, Federal University of Cearÿ, Fortaleza (CE), Brazil
5 Department of Medicine, Medical School of Juazeiro do Norte - Estacio, Juazeiro do Norte (CE), Brazil
6 Department of Health, University of Fortaleza – UNIFOR, Fortaleza (CE), Brazil

Correspondence Address:
Henrique D.M Coutinho
Departamento de Quimica Biologica, Universidade Regional Do Cariri, Av. Cel. Antonio Luiz, 1161, CEP: 63105.000, Crato, CE
Brazil
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-8490.188882

Rights and Permissions

Introduction: Annona coriacea Mart. (araticum) is a widely distributed tree in the cerrado. Its value is attributed principally to the consumption of its fruit which possesses a large nutritive potential. The objective was to identify the chemical profile and evaluate the antimicrobial and cytoprotective activity of the hydroethanol extract of A. coriacea Mart. (HEAC) leaves against the toxicity of mercury chloride. Materials and Methods: The characterization of components was carried out using high-performance liquid chromatography (HPLC). The minimum inhibitory concentration (MIC) was determined by microdilution method in broth with strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. For evaluation of the modulatory and cytoprotective activity of aminoglycoside antibiotics (gentamicin and amikacin) and mercury chloride (HgCl2), the substances were associated with the HEAC at subinhibitory concentrations (MIC/8). Results and Discussion: The HPLC analysis revealed the presence of flavonoids such as Luteolin (1.84%) and Quercetin (1.19%) in elevated concentrations. The HEAC presented an MIC ≥512 μg/mL and significant antagonistic action in aminoglycosides modulation, and it also showed cytoprotective activity to S. aureus (significance P< 0.0001) and E. coli(significance P< 0.05) bacteria against the mercury chloride heavy metal with significance, this action being attributed to the chelating properties of the flavonoids found in the chemical identification. Conclusions: The results acquired in this study show that the HEAC presents cytoprotective activity over the tested strains in vitro and can also present antagonistic effect when associated with aminoglycosides, reinforcing the necessity of taking caution when combining natural and pharmaceutical products.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2385    
    Printed85    
    Emailed0    
    PDF Downloaded18    
    Comments [Add]    

Recommend this journal