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SHORT COMMUNICATION
Year : 2012  |  Volume : 4  |  Issue : 4  |  Page : 230-233

Flower extract of Nyctanthes arbor-tristis modulates glutathione level in hydrogen peroxide treated lymphocytes


Cancer Genetics and Chemoprevention Research Group, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam, India

Correspondence Address:
Anand Ramteke
Cancer Genetics and Chemoprevention Research Group, Department of Molecular Biology and Biotechnology, Tezpur University, Tezpur, Assam - 784 028
India
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-8490.102272

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Background: Nyctanthes arbor-tristis Linn (Oleaceae) is a well-known traditional medicinal plant used throughout the India as an herbal remedy for treating various infectious and non-infectious diseases. Objective: To evaluate the antioxidative activity of hydro-alcoholic extract of flower in the lymphocytes exposed to oxidative stress induced by H 2 O 2 . Materials and Methods: Isolated lymphocytes were treated in vitro with extract or extract+H 2 O 2, and the level of reduced glutathione (GSH) as well as the activity of glutathione-S-transferase (GST) and lactate dehydrogenase (LDH) were measured. Results: Treatment of lymphocyte with flower extract (50, 100, and 200 μg/ ml) significantly increased the level of GSH and decreased the activity of GST. The LDH activity measured in the cell-free medium decreased significantly. Pre-treatment of lymphocyte with flower extract protects the lymphocyte from the H 2 O 2 induced oxidative stress by significantly increasing the levels of GSH as compared to the cells treated only with H 2 O 2 . Pre-treatment also reduced the activity of LDH significantly as compared to the cells treated only with H 2 O 2 . The LDH activity in cell-free medium is associated with membrane damage, the decreased levels of LDH activity reflects the reduced level of membrane damage due to H 2 O 2 . Conclusion: The present findings suggest the protective role of the hydro-alcoholic extracts of the flower of Nyctanthes arbor-tristis against membrane damage induced by H 2 O 2 . The results also suggest that the extract might be rich in phytochemicals with antioxidant/radical scavenging potentials, which might find application in antioxidant therapy.


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