Home | About PR | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |   Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 
SHORT COMMUNICATION
Year : 2012  |  Volume : 4  |  Issue : 1  |  Page : 63-66

Identification of the protease inhibitor miraziridine A in the Red sea sponge Theonella swinhoei


1 University of Wuerzburg, Botany II, Julius-von-Sachs-Institute, Würzburg, Germany
2 University of Wuerzburg, Würzburg, Germany
3 University of Bielefeld, Chemistry, Germany
4 Pharmacy and Food Chemistry, Würzburg; Pharmacy and Biochemistry, Mainz, Germany

Correspondence Address:
Tanja Schirmeister
Institute of Pharmacy and Biochemistry, University of Mainz, Staudinger Weg 5, 55128 Mainz
Germany
Login to access the Email id

Source of Support: German Research Society, financial support, Conflict of Interest: None


DOI: 10.4103/0974-8490.91047

Rights and Permissions

Background : Miraziridine A, a natural peptide isolated from a marine sponge, is a potent cathepsin B inhibitor with a second-order rate constant of 1.5 x 10 4 M -1 s -1 . In the present study, miraziridine A was isolated from the Red Sea sponge Theonella swinhoei on the basis of chromatographic and spectrometric techniques. We conclude that T. swinhoei from the Red Sea represents an alternative source of the aziridinylpeptide miraziridine A to the previously identified Theonella mirabilis from Japan. We confirmed that the metabolite is produced by marine sponges from different geographical locations. Context : Marine sponges have been proven to be a rich source of secondary metabolites exhibiting a huge diversity of biological activities, including antimicrobial, antitumor and immunomodulatory activities. Theonella species (order Lithistida, Demospongiae) have been shown to be a source of anti-protease and anti-HIV secondary metabolites. Aims : To identify the protease inhibitor mirazirine A in the marine sponge Theonella swinhoei. Material and Methods : The marine sponge Theonella swinhoei was collected by SCUBA diving in the Red Sea in Eilat (Israel). Sponge material was lyophilized and further extracted successively with cyclohexane, dichloromethane and methanol to obtain three crude extracts. LC-MS analysis was performed to confirm the presence of Miraziridine A in the dichloromethane fraction. Results : In the present study, miraziridine A was isolated from the Red Sea sponge T. swinhoei on the basis of chromatographic and spectrophotometric techniques. Conclusions : We conclude that T. swinhoei from the Red Sea represents an alternative source of the aziridinylpeptide miraziridine A to the previously identified Theonella mirabilis from Japan.


[FULL TEXT] [PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed2353    
    Printed105    
    Emailed1    
    PDF Downloaded38    
    Comments [Add]    
    Cited by others 2    

Recommend this journal