Home | About PR | Editorial board | Search | Ahead of print | Current Issue | Archives | Instructions | Subscribe | Advertise | Contact us |   Login 
Pharmacognosy Magazine
Search Article 
  
Advanced search 
 


 
 Table of Contents 
ORIGINAL ARTICLE
Year : 2010  |  Volume : 2  |  Issue : 6  |  Page : 325-329  

Secondary metabolites and bioactivities of Myrtus communis


1 Department of Chemistry of Natural Compounds, National Research Centre, Dokki, Cairo, Egypt
2 Department of Pharmacognosy, Faculty of Pharmacy, Cairo University, Cairo, Egypt
3 Department of Pharmacology, National Research Centre, Dokki, Cairo, Egypt

Date of Submission22-Aug-2010
Date of Decision23-Aug-2010
Date of Web Publication12-Jan-2011

Correspondence Address:
Mahmoud I Nassar
Department of Chemistry of Natural Compounds, National Research Centre, 12622 Dokki, Cairo
Egypt
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0974-8490.75449

Rights and Permissions
   Abstract 

Background: Myrtus species are characterized by the presence of phenolic acids, flavonoids, tannins, volatile oils and fatty acids. They are remedies for variety of ailments. This study therefore investigated medicinal effects of Myrtus communis L. Methods: Bioactivity studies of Myrtus communis L. leaves were carried out on volatile oil, 7% methanol and aqueous extracts and the isolated compounds myricetin 3-O-β-glucopyranoside, myricetin 3-O-∝-rhamnopyranoside and gallic acid. Results: Determination of the median lethal dose (LD 50 ) revealed that the volatile oil, alcoholic and aqueous extracts were practically nontoxic and highly safe as no lethality was observed. The tested materials (volatile oil, alcoholic and aqueous extracts, myricetin 3-O-β-glucopyranoside, myricetin 3-O-∝-rhamnopyranoside and gallic acid) showed significant antihyperglycemic, anti-inflammatory and antinociceptive effects as compared with control groups and reference drugs. Conclusion : Administration of extracts of M. communis leaves could be safe at the dose used in this study.

Keywords: Antihyperglycemic, anti-inflammatory, antinociceptive, LD 50 , Myrtus communis, Volatile oil


How to cite this article:
Nassar MI, Aboutabl ESA, Ahmed RF, El-Khrisy EDA, Ibrahim KM, Sleem AA. Secondary metabolites and bioactivities of Myrtus communis. Phcog Res 2010;2:325-9

How to cite this URL:
Nassar MI, Aboutabl ESA, Ahmed RF, El-Khrisy EDA, Ibrahim KM, Sleem AA. Secondary metabolites and bioactivities of Myrtus communis. Phcog Res [serial online] 2010 [cited 2019 Jul 15];2:325-9. Available from: http://www.phcogres.com/text.asp?2010/2/6/325/75449


   Introduction Top


Diabetes mellitus (DM) is a common disorder associated with increased mortality rate and can be identified as a group of metabolic diseases characterized by chronic hyperglycemia resulting from defects in insulin metabolism and impaired function in carbohydrate, lipid and protein metabolism. [1],[2] Myrtaceae is a family comprising at least 133 genera in more than 3800 species. It has a wide distribution in warm climate regions of the world. [3],[4]

Myrtus
is a genus of flowering plants with approximately 16 species of evergreen shrubs or small trees reported in areas of the Middle East and Asia. [5],[6] Myrtus communis L. known as true myrtle and in arabic as mirsin. The plant grows in countries bordering the Mediterranean area and west Asia; it grows spontaneously in Spain, France, Tunisia, Algeria and Morocco. The common myrtle has upright stem, eight or 10-feet high, its branches form a close full head, thickly covered with ovate or lanceolate evergreen leaves; it has solitary axillary white or rosy flowers, followed by black a several-seeded berry which is spherical in shape with dark red to violet in color. Myrtus species were reported as very rich in volatile oils, [7,8] phenolic acids as gallic and ellagic acids, [6] flavonoids, [6,9] fatty acids (FA), [10] tannins [11] and anthocyanin pigments. [12] The present study deals with isolation and identifications of secondary metabolites as well as antihyperglycemic, anti-inflammatory activity, antinociceptive activity and LD 50 as prospective analysis.


   Materials and Methods Top


All the instruments used found at National Research Centre, Cairo.

General

NMR measurements were carried out using JEOL EX -500 spectroscopy; 500 MH Z ( 1 H NMR) and 125 MH Z ( 13 C NMR) and JEOL JNM-EX 270 spectroscopy; 270 MHz ( 1 H NMR) and 67.5 MH Z ( 13 C NMR), mass spectra (±) ESI-MS: LCQ Advantage Thermo Finnigan spectrometer GLC instrument used was Agilent 6890N gas Finnigan- Mat SSQ 7000 spectrometer provided with FID (Flame Ionization Detector). EI ev 70, fused silica capillary column 30-m length, helium gas as a carrier gas; flow-rate (column head pressure 13 PS) and MS detector and UV spectra (OMM 7070E Shimadzu UV 240 spectrophotometer) were run.

Plant materials

M. communis L. leaves were collected from El-Orman garden, Giza, Egypt. The plant samples were kindly identified by Mm. Tressa Labib, Taxonomist, El-Orman garden, Giza, Egypt. The collected samples were air dried, powdered and kept for chemical analysis.

Preparation and GC/MS analysis of volatile oil

Five hundred grams of M. communis leaves was subjected to steam distillation for 5 hr to give yellow oil with pleasant odor and analyzed by GC/MS system equipped with willey 138 and NBS 75 library software was used capillary GC using DB- 5 column. Injection volume was 1.0 ΅l at 1:50 split. Ionization voltage 70 ev scans mass range 30-450, with temperature program 50˚C/5 min, 50-160, 3˚C/min and 160-260, 5˚C/5 min. The essential oil was identified by matching their spectra with those recorded in the MS library and comparison with those of reference compounds.

Preparation of aqueous and alcoholic extracts

The air-dried leaves of M. communis (200 gm) was extracted with warm distilled water for 6 hrs then filtered off to afford 50 gm of the extract, as well as the dried leaves of M. communis (200 gm) was extracted with 70% ethanol for five times to afford 150 gm of the extract.

Preparation and GLC analysis of unsaponifiable matter (USM) and FA.

Petroleum ether extract (15) was saponified to yield the USM fraction (5 gm) and FA fraction (3.9 gm). The FA fraction was methylated by refluxing in 50 ml absolute methanol and 1.5 ml conc. sulphuric acid for 2 hrs and analyzed by GLC. The column used was a capillary column 30-m length, 0.53 mm internal diameter, film thickness 1 μm, packed with HP-INWAX polyethylene glycol. The analysis was carried out at a programmed temperature: initial temperature 100°C (kept for 1 min), then increasing at a rate of 4°C/min and final temperature 220°C (kept for 20 min). Injector temperature was 275°C and detector temperature at 300°C, N2 was as carrier gas at flow-rate 30ml/min For GLC of USM fraction, the column used was a capillary column 30-m length HP-1 methyl siloxane, 530 μm internal diameter 0.26 μm film thickness. The analysis was carried out at a programmed temperature: Initial temperature 60°C for 2 min then increasing at a rate of 10°C/min till 280°C, N2 was used as carrier gas at flow-rate, 30 ml/min for GLC of FA.

Quantitative estimation of total phenolic content in M. communis leaves

The total phenolic content (TPC) of M. communis leaves was determined using Folin-Ciocalteu reagent. [13] In this method the reaction mixture was composed of (100 ΅l) of plant extracts and 500 ΅l of the Folin-Ciocalteu reagent and 1.5 ml of 20% sodium carbonate. The mixture was shaken thoroughly and made up to 10 ml using distilled water; the mixture was allowed to stand for 2 hrs then the absorbance was measured at 765 nm using spectrophotometer. A calibration curve of gallic acid which was dissolved in methanol, water (60: 40 v/v, 0.3% HCl). The content of TPCs of each extract was estimated by comparison with the standard curve generated from gallic acid.

Quantitative estimation of total flavonoids content in M. communis leaves

The total flavonoids content (TF) of each extract was determined spectrophotometerically using rutin as a reference compound. [14] One milliliter of the plant extract in methanol (10 mg/ml) was mixed with 1-ml aluminium trichloride in ethanol (20 mg/ml) and a drop of acetic acid, and then diluted with ethanol to 25 ml the intensity of the developed yellow color was measured at 415 nm. Were taken after 40 min against blank samples (1ml of plant extract and a drop of acetic acid, and then diluted to 25 ml with ethanol). The absorption of standard rutin solution (0.5 mg/ml) in ethanol was measured under the same conditions. All determinations were carried out in triplicate. The amount of flavonoids in M. communis (leaves and fruits) extracts in rutin equivalents (RE) was calculated by the following formula:



Where X = flavonoid content was expressed as milligrams of RE/milligrams of plant extracts.

A = absorbtion of plant extracts solution, A 0 = absorbtion of standard rutin solution, m 0 = weight of standard rutin solution in mg, m = weight of the plant extract in mg.

Isolation of phenolic compounds

The air-dried powder leaves of M. communis (1 kg) were extracted with 70% ethanol. The ethanolic extract was evaporated under reduced pressure to yield 150 g of a brown residue that was suspended in water 1000 ml and partitioned successively with petroleum ether, chloroform, ethyl acetate and n- butanol to afford 15, 20, 70 and 30 gm, respectively. The ethyl acetate extract (70 gm) was subjected to a polyamide 6S column and eluted with distilled water/methanol step gradient. The obtained fractions were inspected by paper chromatography using BAW and 15% Ac.OH as developing systems. The similar fractions were collected together and subjected to Sephadex LH-20 afforded gallic acid (80 mg), methyl gallate (10 mg), myricetin-3-O-β-glucoside (60 mg), myricetin-3-O-α-rhamnopyranoside (65 mg), quercetin-3-O-β-galactoside (6 mg), quercetin-3-O-β-glucopyranoside (4mg), quercitrin (6 mg), ellagic acid (40 mg), myricetin (15 mg) and quercetin (10 mg).

Animals

Albino mice of 25-30 gm body weight and adult male Albino rats of Sprague Dawely Strain of 130-150 gm body weight were used in this study, obtained from the animal house colony of National Research Centre, Egypt. All animals were kept on a standard laboratory diet under the same hygienic conditions.

Chemicals and kits

Metformin (Chemical Industries Development, Giza, A.R.E), alloxan (Sigma Co: Cairo,Egypt.) Biodiagnostic kit for assessment of blood glucose and glutathione levels, indomethacin (Epico, Egyptian Int. Pharmaceutical Industries Co., A.R.E.). Carrageenan Sigma Co. Tramadol; (Sigma Co.). All other chemicals used in the experimental work were in analytical grade.

Doses of the tested materials and drugs were administered orally by gastric tube. [15]

Pharmacological screening

Median Lethal Dose (LD 50 ): determination of the LD 50 of extracts and pure compounds of M. communis leaves was estimated where all doses were expressed in terms of extract weight/ animal weight. [16] Preliminary experiments were done to determine the minimal dose that kills all animals (LD 100 ) and the maximal dose that fails to kill any animal. Several doses at equal logarithmic intervals were chosen in between these two doses, each dose was injected in a group of six animals by subcutaneous injection. The mice were observed for 24 hrs and symptoms of toxicity and mortality rates in each group were recorded and the (LD 50 ) was calculated.

Antihyperglycemic activity
: Male albino rats of the Sprague Dawely Strain (130-140 g) were injected intra-peritoneal with alloxan (150 mg/kg body weight) to induce DM. [17] Hyperglycemia was assessed after 72 hrs by measuring blood glucose and after 2 and 4-week intervals. Animals were divided into seven groups, first group: diabetic rats that served as control, second group: rats that received 100 mg/kg of the aqueous extract, third group: rats that received 100 mg/kg of the alcoholic extract, fourth group: rats that received 100 mg/kg of the myrecetin 3-O-glucoside, fifth group: rats that received 100 mg/kg of the myrecetin 3-O-rhamnoside, sixth group: rats that received 100 mg/kg of the gallic acid, seventh group: diabetic rats that received 150 mg/kg b.wt. of metformin drug as reference drug. At the end of each study period, blood samples were collected from the retro-orbital venous plexus through the eye canthus of anesthetized rats after an overnight fast. Serum was isolated by centrifugation and the blood glucose level was measured. [18]

Anti-inflammatory activity: This effect was determined according to the method described by Winter et al. [19] Forty-eight male albino rats, weighing 130-150 gm were divided into eight groups, each of six animals, first group: rats that received 1 ml of saline serving as control, second group: rats that received 100 mg/kg of the aqueous extract, third group: rats that received 100 mg/kg of the alcoholic extract, fourth group: rats that received 0.1ml/kg of the oil, fifth group: rats that received 100 mg/kg of the myrecetin 3-O-glucoside, sixth group: rats that received 100 mg/kg of the myrecetin 3-O-rhamnoside, seventh group: rats that received 100 mg/kg of the gallic acid, eighth group: rats that received 20 mg/kg of the reference drug, indomethacin. One hour later, all the animals received a sub plantar injection of 0.1 ml of 1% carrageenan solution in saline, in the right hind paw and 0.1 ml saline in the left hind paw. Four hours after drug administration, the rats were sacrificed; both hind paws excised and weighed separately.



Antinociceptive activity: Animals were acclimatized to the laboratory conditions for at least 1 hr before testing and were used once during the experiment.

Acetic acid-induced writhing test: The first group received acetic acid, second, third, fourth, fifth, sixth and seventh groups received the extracts in the aforementioned doses, and 30 min later 0.6% acetic acid was injected i.p. (0.2 ml /mice). Each mice was then placed in an individual clear plastic observe chamber and 1 total no of writhes/30 min was counted for each mouse. [20]

Statistical analysis

The obtained data were analyzed by using the Student's t test. [21]


   Results and Discussion Top


Nineteen compounds were identified in the volatile oil of M. communis by using GC/MS analysis [Table 1]. 1, 8-Cineol (27.19%), α-pinene (25.53%), linalool (11.75%) represent the major constituents. Saponfication of the petroleum ether extract afforded the FA fraction, as well as unsaponfiable matter (USM) fraction. GLC analysis of FA revealed the presence of 11 compounds. The total identified saturated FAs (47.19%) was higher than that of unsaturated FAs (52.1%); palmitic and arachidic acids were the major saturated FAs (13.9% and 13.5%, respectively); oleic and linolenic acids were the major unsaturated FAs (18.4% and 11.4%, respectively). GLC of USM fraction revealed the presence of 19 compounds. The total identified compounds representing (97.6%) and involved cholesterol (17.26%), β-sitosterol (6.69%), in addition to long-chain hydrocarbons. Column chromatography of M. communis leaves lead to the isolation of gallic acid, [22] methyl gallate, [23] myricetin-3-O-β-glucoside, [24] myricetin-3-O-α-rhamnopyranoside, [25] quercetin-3-O- β-galactoside, [26],[27] quercetin-3-O-β-glucopyranoside (4 mg), [28] quercitrin, [29] ellagic acid, [30] myricetin [31],[32] and quercetin. [31] The TPC in M. communis leaves (mg gallic acid equivalent/gram of plant extract) in alcoholic, chloroformic and ethyl acetate extracts was 472.47 ± 3.73, 346.89 ± 7.56 and 714.33 ± 4.69, respectively, while the TF content in M. communis leaves (mg RE/ gm of plant extract) in alcoholic, chloroformic and ethyl acetate extracts was 281.15 ± 21.88, 44.78 ± 8. 98 and 153.62 ± 13.27, respectively.
Table 1 :GC/MS analysis of the volatile oil from the leaves of Myrtus communis

Click here to view


Bioassay

Study of the acute toxicity of the volatile oil, aqueous and alcoholic extracts of M. communis leaves were safe and their LD 50 were 6.4, 10 and 10 gm/kg, respectively. The antihyperglycemic activity of volatile, aqueous and alcoholic extracts, myricetin-3-O- glucoside, myricetin- 3-O- rhamnoside and gallic acid are presented in [Table 2] with percentage 42.7, 1.5, 54, 41.3, 35.3 and 34.4%, respectively. It could be deduced that the alcoholic extract exhibited the highest antihyperglycemic activity as compared with the control. Results of the anti-inflammatory activity of volatile oil, aqueous and alcoholic extracts, myricetin-3-O- glucoside, myricetin- 3-O- rhamnoside and gallic acid [Table 3] with percentage 59.4, 50.7, 56.6, 53.9, 46.8 37.4%, respectively. It could be concluded that, the volatile oil exhibited the highest anti-inflammatory activity compared with indomethacin used as a reference drug. Antinociceptive activity of volatile oil, aqueous and alcoholic extracts, myricetin-3-O- glucoside, myricetin- 3-O- rhamnoside and gallic acid [Table 4] with percentage 50, 46.1, 51, 40.9, 35.6 and 26.7%, respectively.[33]
Table 2 :Antihyperglycemic activity of aqueous and alcoholic extracts, volatile oil and isolated compounds of Myrtus communis leaves on blood glucose level in male albino rats (n=10)

Click here to view
Table 3 :Acute anti-inflammatory activity of aqueous, alcoholic extracts, volatile oil and certain compounds isolated from Myrtus communis leaves in comparison with indomethacin in male albino rats (n=6)

Click here to view
Table 4 :Antinociceptive activity of aqueous and alcoholic extracts, volatile oil and isolated compounds of Myrtus communis leaves on number of abdominal constrictions in mice (n=6)

Click here to view


 
   References Top

1.Lebovitz HE. Oral antidiabetic agents. In: Kahn CR, Weir GC, editors. Joslin`s Diabetes Mellitus. 13th ed., Vol. 29. Philadelphia: Lea and Febiger; 1994. p. 508-524.  Back to cited text no. 1
    
2.Andreoli TE, Carpenter CC, Plum F, Smith LH. Diabetes mellitus. In: Dyson J. (Ed.). Cecil Essential of medicine. Vol. 1. WB Saunders, Philadelphia;1990. p. 559-66.  Back to cited text no. 2
    
3.Hora FB. Flowering Plants of the World. UK: Oxford University Press Oxford; 1997.   Back to cited text no. 3
    
4.Evans WC. Pharmacognosy. 15 th ed. London, UK: WB Saunders Company Limited; 2002.   Back to cited text no. 4
    
5.Twaij HA, Ali HM, Al-Zohyri AM. Phytochemical and antimicrobial studies of Myrtus communis. J Biol Sci Res 1988;19:29-39.   Back to cited text no. 5
    
6.Romani A, Pinelli P, Mulinacci N, Vincieri FF, Tattini M. Identification and quantification of polyphenols in leaves of Myrtus communis. Chromatographia 1999;49:17-20.  Back to cited text no. 6
    
7.Satrani B, Farah A, Talbi M. Fractional distillation effect on the chemical composition and antimicrobial activity of Moroccan Myrtle. Acta Bot Gallica 2006;153:235-242.  Back to cited text no. 7
    
8.Shikhiev AS, Abbasov RM, Mamedova ZA. Composition of Myrtus communis essential oil. Khimiya Prirodnykh Soedinenii 1978;4:529-530.  Back to cited text no. 8
    
9.Joseph MI, Pichon PN, Raynaud J. Flavonoid heterosides of the leaves of Myrtus communis L. (Myrtaceae). Pharmazie 1987;42:142.  Back to cited text no. 9
    
10.Cakir A. Essential oil and fatty acid composition of the fruits of Hippophae rhamnoides L. and Myrtus communis L. from Turkey. Biochemical Systematics and Ecology 2004;32:809-816.  Back to cited text no. 10
    
11.Diaz AM, Abeger A. Study of the polyphenolic compounds present in alcoholic extracts of Myrtus communis L. seeds. An Real Acad Farm 1986;52:541-546.  Back to cited text no. 11
    
12.Martin T, Villaescusa L, De Sotto M, Lucia A, Diaz AM. Determination of anthocyanin pigments in Myrtus communis berries. Fitoterapia 1990;61:85.  Back to cited text no. 12
    
13.Yu L, Hayley S, Peut J, Harris M, Wilson J, Qian M. Free radical scavenging properties of wheat extracts. J Agric Food Chem 2002;50:1619-1624.  Back to cited text no. 13
    
14.Kumaran A, Karunakaran J. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT 2006;40:344-352.  Back to cited text no. 14
    
15.Paget G, Berne's E. Toxicity tests in evaluation of drugs activities cited in the laboratory rat. London: Academic Press; 1964. p. 135-160.  Back to cited text no. 15
    
16.Karber G. Determination of median leathal dose. Arch Exp Pathol Pharmacol 1931;162:480-487.  Back to cited text no. 16
    
17.Eliasson SG, Samet TM. Alloxan induced neuropathies lipid changes in nerve and root fragments. Life Sci 1969;8:493-498.  Back to cited text no. 17
    
18.Trinder P. Estimation of serum glucose and triglycerides by enzymatic method. Am Clin Biochem 1969;6:24.   Back to cited text no. 18
    
19.Winter GA, Risley EA, Nuss GW. Carrageenane induced oedema in hind paw of the rat as an assay for anti-inflammatory drugs. Biol Med 1962;111:1544-1547.   Back to cited text no. 19
    
20.Koster R, Anderson M, Deber J. Method for determination of analgesic activity. Fed Proc 1959;18:412.  Back to cited text no. 20
    
21.Barakat HH, Nawwar MA, Buddrus J, Linsheid M. Niloticol, a phenolic glyceride and two phenolic aldehydes from the roots of Tamarix nilotia. Phytochemistry 1987;26:1837-838.  Back to cited text no. 21
    
22.Spedecor WG, Choehran GW. Statistical methods for the determination of blood glutathione. J. Lab. Clin. Med. 1982;61:882-888;   Back to cited text no. 22
    
23.Smith I. Chromatographic and electrophoretic techniques. Heinman, London. 1960.  Back to cited text no. 23
    
24.Ca-Jane S, Chien-Kuang C, Shoei-Sheng L. Polar constituents from Sageretia thea leaf characterized by HPLC-SPE-NMR. J Chin Chem Soc 2009;56:1002-1009.  Back to cited text no. 24
    
25.Marco JA, Badera O, Sanz JF, Sanchez-Parareda J. Flavonol glycosides from Anthyllis onobrychioides. Phytochemistry 1985;36:793-98.   Back to cited text no. 25
    
26.Yasukawa K, Takido M. A flavonol glycoside from Lysimachia mauritiana. Phytochemistry 1987;26:1224.  Back to cited text no. 26
    
27.Mahmoud II, Marzouk MS, Moharram FA, Nolte J, Fobbe R, Saleh MI. Polyphenolic constituents of Callistemon lanceolatus leaves. Pharmazie 2002;57:494-496.  Back to cited text no. 27
    
28.Nawwar MA, El-mosallamy AM, Barakat HH. Quercetin 3-glucosides from leaves of Solanum Nigrum. Phytochemistry 1989;28:1755-1757.  Back to cited text no. 28
    
29.Nawwar MA, Ishak MS, Michael HN, Buddrus J. Leaf flavonoids of Ziziphus spina-christi. Phytochemistry 1984;23:2110-2111.  Back to cited text no. 29
    
30.Nawwar MA, Hussein SA, Merfort I. NMR spectral analysis of polyphenols from Puica granatum. Phytochemistry 1994;36:793-798.  Back to cited text no. 30
    
31.Harborne JB. The Flavonoids, Advances in Research. London: Chapman and Hall; 1982.  Back to cited text no. 31
    
32.Yang SW, Zhou BN, Wise JH, Evans R, Van der Werff H, Miller JS, et al. Three new ellagic acid derivatives from the bark of Eschweilera coriacea from the Suriname Rainforest. J Nat Prod 1998;61:901-906.  Back to cited text no. 32
    
33.Adams RP. Identification of volatile oil components by gas chromatography/ mass spectroscopy, Allured Publishing Cooperation USA;1995.  Back to cited text no. 33
    



 
 
    Tables

  [Table 1], [Table 2], [Table 3], [Table 4]


This article has been cited by
1 Myrtucomvalones A–C, three unusual triketone–sesquiterpene adducts from the leaves of Myrtus communis ‘Variegata’
Ming Chen,Li-Feng Chen,Man-Mei Li,Ni-Ping Li,Jia-Qing Cao,Ying Wang,Yao-Lan Li,Lei Wang,Wen-Cai Ye
RSC Adv.. 2017; 7(37): 22735
[Pubmed] | [DOI]
2 The GenusMyrtusL. in Algeria: Composition and Biological Aspects of Essential Oils fromM. communisandM. nivellei: A Review
Amel Bouzabata,Joseph Casanova,Ange Bighelli,Carlos Cavaleiro,Ligia Salgueiro,Félix Tomi
Chemistry & Biodiversity. 2016; 13(6): 672
[Pubmed] | [DOI]
3 Myrtus communis L. and its application in treatment of Recurrent Aphthous Stomatitis
Mohaddese Mahboubi
Journal of Ethnopharmacology. 2016; 193: 481
[Pubmed] | [DOI]
4 Therapeutic Potential ofMyrtus communisSubsp.communisExtract Against Acetic ACID-Induced Colonic Inflammation in Rats
Ali Sen,Meral Yuksel,Gizem Bulut,Leyla Bitis,Feriha Ercan,Nagehan Ozyilmaz-Yay,Ozben Akbulut,Hamit Cobanoglu,Sevil Ozkan,Goksel Sener
Journal of Food Biochemistry. 2016;
[Pubmed] | [DOI]
5 Profiling and Simultaneous Quantitative Determination of Anthocyanins in WildMyrtus communisL. Berries from Different Geographical Areas in Sardinia and their Comparative Evaluation
Mariateresa Maldini,Mario Chessa,Giacomo L. Petretto,Paola Montoro,Jonathan P. Rourke,Marzia Foddai,Marcello Nicoletti,Giorgio Pintore
Phytochemical Analysis. 2016;
[Pubmed] | [DOI]
6 Antibacterial Activity of Some Plant Extracts Against Extended- Spectrum Beta-Lactamase Producing Escherichia coli Isolates
Saeide Saeidi,Negar Amini Boroujeni,Hassan Ahmadi,Mehdi Hassanshahian
Jundishapur Journal of Microbiology. 2015; 8(2)
[Pubmed] | [DOI]
7 Phytochemical analysis, hypotensive effect and antioxidant properties of Myrtus communis L. growing in Algeria
Amel Bouaziz,Seddik khennouf,Mussa Abu zarga,Shtaywy Abdalla,Abderahmane Baghiani,Noureddine Charef
Asian Pacific Journal of Tropical Biomedicine. 2015; 5(1): 19
[Pubmed] | [DOI]
8 Identification of Phenolic Compounds in Petals of Nasturtium Flowers (Tropaeolum majus) by High-Performance Liquid Chromatography Coupled to Mass Spectrometry and Determination of Oxygen Radical Absorbance Capacity (ORAC)
G. Astrid Garzón,David C. Manns,Ken Riedl,Steven J. Schwartz,Olga Padilla-Zakour
Journal of Agricultural and Food Chemistry. 2015; 63(6): 1803
[Pubmed] | [DOI]
9 Myrtus communis L. as source of a bioactive and safe essential oil
Amel Bouzabata,Célia Cabral,Maria José Gonçalves,Maria Teresa Cruz,Ange Bighelli,Carlos Cavaleiro,Joseph Casanova,Félix Tomi,Ligia Salgueiro
Food and Chemical Toxicology. 2014;
[Pubmed] | [DOI]
10 Synergistic effect of Myrtus communis L. essential oils and conventional antibiotics against multi-drug resistant Acinetobacter baumannii wound isolates
Verica Aleksic,Neda Mimica-Dukic,Natasa Simin,Natasa Stankovic Nedeljkovic,Petar Knezevic
Phytomedicine. 2014; 21(12): 1666
[Pubmed] | [DOI]
11 Review of Pharmacological Effects ofMyrtus communisL. and its Active Constituents
Ghazal Alipour,Saeedeh Dashti,Hossein Hosseinzadeh
Phytotherapy Research. 2014; : n/a
[Pubmed] | [DOI]
12 The phenolic content and antioxidant activity of infusions from Mediterranean medicinal plants
Gonçalves, S. and Gomes, D. and Costa, P. and Romano, A.
Industrial Crops and Products. 2013; 43(1): 465-471
[Pubmed]
13 Antimicrobial and antioxidative activity of extracts and essential oils of Myrtus communis L.
Verica Aleksic,Petar Knezevic
Microbiological Research. 2013;
[Pubmed] | [DOI]
14 The phenolic content and antioxidant activity of infusions from Mediterranean medicinal plants
Sandra Gonçalves,Diogo Gomes,Patrícia Costa,Anabela Romano
Industrial Crops and Products. 2013; 43: 465
[Pubmed] | [DOI]
15 Antiplasmodial activity of ethanolic extracts of some selected medicinal plants from the northwest of Iran
Hadi Sangian,Hossein Faramarzi,Alireza Yazdinezhad,Seyed Javad Mousavi,Zahra Zamani,Maryam Noubarani,Ali Ramazani
Parasitology Research. 2013; 112(11): 3697
[Pubmed] | [DOI]
16 Anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the Mediterranean area
Mario Dell’Agli,Cinzia Sanna,Patrizia Rubiolo,Nicoletta Basilico,Elisa Colombo,Maria M Scaltrito,Mamadou Ndiath,Luca Maccarone,Donatella Taramelli,Carlo Bicchi,Mauro Ballero,Enrica Bosisio
Malaria Journal. 2012; 11(1): 219
[Pubmed] | [DOI]
17 Anti-plasmodial and insecticidal activities of the essential oils of aromatic plants growing in the Mediterranean area
Dellagli, M. and Sanna, C. and Rubiolo, P. and Basilico, N. and Colombo, E. and Scaltrito, M.M. and Ndiath, M.O. and MacCarone, L. and Taramelli, D. and Bicchi, C. and Ballero, M. and Bosisio, E.
Malaria Journal. 2012; 11(219)
[Pubmed]
18 Comparative study of essential oils extracted from Algerian myrtus communis L. leaves using microwaves and hydrodistillation
Berka-Zougali, B. and Ferhat, M.-A. and Hassani, A. and Chemat, F. and Allaf, K.S.
International Journal of Molecular Sciences. 2012; 13(4): 4673-4695
[Pubmed]
19 Comparative Study of Essential Oils Extracted from Algerian Myrtus communis L. Leaves Using Microwaves and Hydrodistillation
Baya Berka-Zougali,Mohamed-Amine Ferhat,Aicha Hassani,Farid Chemat,Karim S. Allaf
International Journal of Molecular Sciences. 2012; 13(12): 4673
[Pubmed] | [DOI]



 

Top
  
 
  Search
 
    Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
    Access Statistics
    Email Alert *
    Add to My List *
* Registration required (free)  

 
  In this article
    Abstract
    Introduction
    Materials and Me...
    Results and Disc...
    References
    Article Tables

 Article Access Statistics
    Viewed5545    
    Printed202    
    Emailed1    
    PDF Downloaded22    
    Comments [Add]    
    Cited by others 19    

Recommend this journal